The tumor microenvironment (TME) plays an important role in the pathogenesis of many cancers. We aimed to screen TME-related hub genes colorectal adenoma (CRAD) and identify possible prognostic biomarkers. gene expression profiles clinical data 464 CRAD patients Cancer Genome Atlas (TCGA) database were downloaded. Estimation STromal Immune cells MAlignant Tumours using Expression (ESTIMATE) algorithm was performed calculate ImmuneScore, StromalScore, EstimateScore. Thereafter, differentially...

Abstract Background Interleukin-32 (IL-32) is an important factor in innate and adaptive immune responses, which activates the p38MAPK, NF-kappa B AP-1 signaling pathways. Recent reports have highlighted that IL-32 regulated during viral infection humans. Methods Enzyme-linked immunosorbent assays (ELISA) were carried out to detect levels serum samples. Detailed kinetics of transcription mRNA expression protein human cytomegalovirus (HCMV) determined by semi-quantitative RT-PCR western blot,...

It has been reported that human cytomegalovirus (HCMV) miR‐US25‐2 reduces DNA viral replication including HCMV. However, the mechanism remains unknown. In our study, eukaryotic translation initiation factor 4A1 ( eIF4A1 ) was identified to be a direct target of miR‐US25‐2‐3p. Small interfering RNA (siRNA) and miR‐US25‐2‐3p mediated knockdown experiments revealed high level in MRC‐5 cells decreased HCMV host genomic synthesis, inhibited cap‐dependent cell proliferation. up‐regulation induced...

Abstract Background microRNAs (miRNAs) are a group of regulatory RNAs that regulate gene expression by binding to specific sequences on target mRNAs. However, functional identification mRNA targets is usually difficult and time consuming. Here we report hybrid-PCR as new rapid approach screen putative in vitro. Results Fifteen mRNAs for human cytomegalovirus (HCMV) miR-UL112-1, including previously confirmed HCMV IE72, were identified from mRNA-derived cDNAs using hybrid-PCR. Moreover,...

Human cytomegalovirus (HCMV) is a herpesvirus that causes congenital diseases and opportunistic infections in immunocompromised individuals. Its functional proteins microRNAs (miRNAs) facilitate efficient viral propagation by altering host cell behavior. The identification of target genes miRNAs an important step the study HCMV pathogenesis. encodes at least 14 miRNAs, including hcmv-mir-UL148D, which resides UL/b' region. hcmv-mir-UL148D only miRNA encoded region; however, its targets...

Persistent high-risk human papillomavirus (HPV) infection is correlated with an increased risk of developing intraepithelial lesion or malignancy (NILM). The aims the current study to establish a method named BioPerfectus Multiplex Real Time (BMRT) HPV assay for simultaneous typing and quantifying HPVs, evaluate it by comparison GenoArray test PCR-sequencing method, as well histological status. A total 817 cervical specimens were evaluated BMRT test, using reference standard; simultaneously,...

Abstract Background High-risk human papillomavirus type 16 (HPV16) is a risk factor for cervical cancer. Previous studies suggest that polymorphisms in the E6 gene or long control region(LCR)of HPV16 may alter oncogenic potential of virus. The aims this study were to investigate genetic variations and LCR isolates from Chinese population correlation with disease status infected patients. Methods positive endocervical specimens collected 304 women living Northeast China. Sequences analyzed by...

During infection with human cytomegalovirus (HCMV), overexpression of hcmv‐miR‐US33 can inhibit the lytic viral replication and down‐regulate US29 mRNA. However, it remains unknown whether inhibition by miR‐US33 is mediated down‐regulation expression or another host gene. Here, we identified gene Syntaxin3 ( STX3 ) to be a direct target hcmv‐miR‐US33‐5p using Hybrid‐PCR luciferase‐reporter assays. It was further demonstrated that levels protein were down‐regulated in...

Gut microbiota is an emerging field of research, with related research having breakthrough development in the past 15 years. Bibliometric analysis can be applied to analyze evolutionary trends and hotspots this field.To study subject knowledge structures gut fields from 2004 2018.The literature data on were identified downloaded PubMed database. Through biclustering analysis, strategic diagrams, social network main trend structure concerning analyzed obtain compare each period.According...

The human cytomegalovirus (HCMV) encodes ≥26 microRNAs (miRNAs). These miRNAs are utilized by HCMV to regulate its own genes in addition the of host cell, during infection. present study first identified p21‑activated kinase 2 (PAK2) as a target hcmv‑miR‑US4‑5p, via hybrid polymerase chain reaction, which was further verified using luciferase reporter assay. protein expression level PAK2, detected western blotting, demonstrated be directly downregulated overexpression hcmv‑miR‑US4‑5p HEK293,...

<i>Objectives:</i> Human cytomegalovirus (HCMV) infects a number of organs and cell types in vivo. The different symptoms tissue tropisms HCMV infection perhaps result from the genetic polymorphism. A new region DNA containing at least 19 open reading frames (ORFs – denoted UL133–151) was found low-passage clinical strain Toledo several other isolates, but not present laboratory AD169. Two these genes, UL146 UL147, encode proteins with sequence characteristics CXC (α) chemokines,...

Human papillomavirus (HPV) 52 is an oncogenic HPV type prevalent in Asia. The aim of the study was to analyze genetic variations women from Northeast China. To explore intratypic variants 52, genomic regions L1, E6, E7 and long control region (LCR) which have been identified China by GenoArray test, were analyzed. Twenty-five mutations examined. Of found L1 gene, three novel nonsynonymous C5640T, A5641T G5642A located within that encodes binding domain neutralizing antibodies against 52....

Cervical cancer is the fourth leading cause of cancer-associated mortality worldwide. However, its underlying molecular mechanisms are unclear. It important to explore these in order identify novel diagnostic and prognostic biomarkers. The present study determined association between STAT1 human papillomavirus (HPV)16 cervical lesions. expression was detected by immunohistochemistry. Quantitative PCR used detect HPV16 viral load potential associations among expression, severity lesions...

Human cytomegalovirus (HCMV) infects a number of organs and cell types, leading to the hypothesis that HCMV disease tissue tropism may be related specific sequence variability. This study examined genomic variability new polymorphic locus in HCMV, UL139 open reading frame (ORF). Detailed analysis showed large nucleotide insertions non-synonymous substitutions occurred ORF, particularly 5' half, using Toledo strain as reference sequence. The variants were not distributed randomly, but...

The functions of some proteins encoded by human cytomegalovirus (HCMV) UL/b' genes have been studied; however, systematic analysis the transcripts for this region is still insufficient. results both rapid amplification cDNA ends (RACE) and library screening in study proved that 3' termini all UL138-UL145 were located approximately 20 bp downstream from each potential poly (A) signal, which at positions nucleotides 7184, 9954 12848 sequence H strain, respectively. Thus, there least two large...