Abstract Background Dental pulp stem cells (DPSCs) are a promising cell source in endodontic regeneration and tissue engineering with limited self-renewal pluripotency capacity. N 6 -methyladenosine (m A) is the most prevalent, reversible internal modification RNAs associated fate determination. In this study, we aim to explore biological effect of m A methylation DPSCs. Methods immunoprecipitation deep sequencing RIP-seq) demonstrated features modifications DPSC transcriptome. Lentiviral...

Abstract Background Long noncoding RNAs (lncRNAs) play an important role in the multiple differentiations of mesenchymal stem cells (MSCs). However, few studies have focused on regulatory mechanism lncRNAs odontogenic differentiation human dental pulp (hDPSCs). Methods hDPSCs were induced to differentiate into odontoblasts vitro, and expression profiles lncRNAs, microRNAs (miRNAs), messenger (mRNAs) differentiated undifferentiated obtained by microarray. Bioinformatics analyses including...

Dental pulp regeneration is significantly aided by human dental stem cells (hDPSCs). An increasing number of studies have demonstrated that circular RNAs (circRNAs) are crucial in the multidirectional differentiation many mesenchymal cells, but their specific functions and mechanisms remain unknown. This work aimed at elucidating molecular mechanism which hsa_circ_0001599 works hDPSCs during odontogenic differentiation. The expression tissue was determined using quantitative real-time PCR...

This study aimed to analyse the relationship of blood lipid profile and interleukin-6 (IL-6) with osteoporosis osteopenia explore predictive value combined application these biomarkers in osteopenia. Data from 276 patients treated orthopaedics department were retrospectively analysed. Their general information was collected, relationships among profile, IL-6 bone turnover markers, mineral density (BMD) Patients categorized based on their T scores for intergroup comparisons. Finally,...

Alternative splicing not only expands the genetic encoding of genes but also determines cellular activities. This study aimed to elucidate regulation mechanism and biological functions lincRNA-ASAO in process odontogenesis-related alternative mediated odontogenic differentiation hDPSCs. RACE, RNA-seq, FISH bioinformatics techniques were used identify novel lincRNA-ASAO. ALP staining, alizarin red qRT-PCR western blot role regulating odontoblast The binding protein PTBP1 was screened by...

Abstract Aim Dental pulp stem cells (DPSCs) are essential for regeneration but face low survival rates after transplantation. Genetic modification before transplantation is a promising solution to this issue. We aim elucidate the biological function and regulatory mechanism of hypoxic lncRNA HRL‐SC in DPSCs. Methodology The functions hypoxia inducible factor‐1α (HIF‐1α) DPSCs were evaluated vitro by cell proliferation, migration tube formation assays. Subcutaneous nude mice was used evaluate...

Abstract Background Pulpitis is an inflammatory disease, the grade of which classified according to level inflammation. Traditional methods evaluating status dental pulp tissue in clinical practice have limitations. The rapid and accurate diagnosis pulpitis essential for determining appropriate treatment. By integrating different datasets from Gene Expression Omnibus (GEO) database, we analysed a merged expression matrix pulpitis, aiming identify biological pathways diagnostic biomarkers...

Introduction . Odontogenic differentiation of human dental pulp stem cells (hDPSCs) is a key step regeneration. Recent studies showed that circular RNAs (circRNAs) have many biological functions and competing endogenous RNA (ceRNA) their most common mechanism action. However, the role circRNAs in hDPSCs during odontogenesis still unclear. Methods Isolated were cultured essential odontogenic medium. Total was extracted after 14 days culture, then, microarray analysis performed to measure...

Abstract Background Cone-beam computed tomography (CBCT) was employed to study the morphology and curvature of middle mesial canals (MMCs) mandibular first molars (MFMs). Methods CBCT scanning performed on MFMs 1100 patients. Patients' images that met inclusion criteria were divided into group A (< 40 years old) B (≥ for further study. The used incidence MMCs at different ages, measure in mesiodistal buccolingual directions using Schneider method, observe anatomical root canal system. All...

Human dental pulp stem cells (hDPSCs) are critical for generation. hDPSCs proliferate faster under hypoxia, but the mechanism by which long noncoding RNA (lncRNA) regulates this process is not fully understood.Novel lncRNAs were obtained reanalysis of transcriptome datasets from RNA-Seq hypoxia compared with normoxia, and a differential expression analysis target genes was performed. Bioinformatics analyses, including gene ontology analysis, Kyoto Encyclopedia Genes Genomes pathway set...

Cone-beam computed tomography (CBCT) was used to study the root canal system of mandibular anteriors (MAs) in a Cantonese population and evaluate correlation between complicated configurations lateral incisors (MLIs) presence distolingual roots (DLRs) first molars (MFMs).A total 11,376 anterior teeth were scanned by CBCT. Those whose images met inclusion criteria analysed according Vertucci's configuration then grouped based on gender, age, side, their effects morphology analysed. Finally,...

Background: Human dental pulp stem cells (hDPSCs) play an important role in endodontic regeneration. N6-methyladenosine (m6A) is the most common RNA modification, and noncoding RNAs have also been demonstrated to regulatory roles expression of m6A proteins. However, study on modification hDPSCs has not yet conducted. Methods: Single base site PCR (MazF) was used detect lncSNHG7 before after mineralization screen target protein, bioinformatics analysis analyze related pathways rich lncSNHG7....

To investigate mesial root canal curvatures of human mandibular first molars using cone-beam computed tomography (CBCT).CBCT was performed for scanning the 1600 Chinese subjects, among whom subjects identified to have middle canals were divided into 5 age groups, namely group A (18-29 years), B (30-39 C (40-49 D (50-59 and E (60-80 years) further analysis. All CBCT images reconstructed Planmeca Romexis image reconstruction system, curvature measured Schneider method.A total 2856 obtained...

Abstract Background: Human dental pulp stem cells (hDPSCs) play an important role in endodontic regeneration, with self-renewal and pluripotency capacity. N6-methyladenosine (m 6 A) is the most common RNA modification, noncoding RNAs have also been demonstrated to regulatory roles expression of m A proteins. This study aimed explore mechanism methyltransferase 3 (METTL3)-mediated long (lncRNA) modification osteogenic differentiation hDPSCs. Methods: Single base site PCR (MazF) was used...

Abstract Background: Cone-beam computed tomography (CBCT) was employed to study the morphology and curvature of middle mesial canals (MMCs) mandibular first molars (MFMs). Methods: CBCT scanning performed on MFMs 1100 patients. Patients' images that met inclusion criteria were divided into group A (<40 years old) B (≥40 for further study. The used incidence MMCs at different ages, measure in mesiodistal buccolingual directions using Schneider method, observe anatomical root canal system....

Abstract Background： Cone-beam computed tomography (CBCT) was used to study the morphology and curvature of middle mesial canals (MMCs) mandibular first molars (MFMs). Methods： CBCT scanning performed on MFMs 1100 patients. The pa tients' images which met inclusion criteria were divided into group A (<40 years old) B (≥40 for further study. To incidence MMCs at different ages, measure mesiodistal buccolingual direction by Schneider method, observe anatomical root canal system. Results： In...

Pulp revascularization has become a new method for the treatment of periapical diseases in young permanent teeth recent years. Through root canal flushing and disinfection, avoiding mechanical preparation, guiding apical stem cells into promoting continuous development tooth roots, it achieved good clinical curative effects. But adult patients with chronic periodontitis immature roots open apices, barrier technology is often used to treat these teeth.Pulp 26-year-old patient's was performed...

Abstract Background: Pulpitis is an inflammatory disease, the grade of which classified according to level inflammation. Traditional methods evaluating status dental pulp tissue in clinical practice have limitations. The rapid and accurate diagnosis pulpitis essential for determining appropriate treatment. By integrating different datasets from Gene Expression Omnibus (GEO) database, we analysed a merged expression matrix pulpitis, aiming identify biological pathways diagnostic biomarkers...

Abstract Background: Pulpitis is an inflammatory disease, the grade of which classified according to level inflammation. Traditional methods evaluating status dental pulp tissue in clinical practice have limitations. The rapid and accurate diagnosis pulpitis essential for determining appropriate treatment. By integrating different datasets from Gene Expression Omnibus (GEO) database, we analysed a merged expression matrix pulpitis, aiming identify biological pathways diagnostic biomarkers...

Abstract Background: Cone-beam computed tomography (CBCT) was employed to study the morphology and curvature of middle mesial canals (MMCs) mandibular first molars (MFMs). Methods: CBCT scanning performed on MFMs 1100 patients. Patients' images that met inclusion criteria were divided into group A (<40 years old) B (≥40 for further study. The used incidence MMCs at different ages, measure in mesiodistal buccolingual directions using Schneider method, observe anatomical root canal system....

Abstract Background Pulpitis is known as an inflammatory disease classified by the level of inflammation. The existed traditional methods evaluating status dental pulp tissue in clinical practice still have some shortages and limitations. Immediate accurate diagnosis pulpitis essential to choice treatment. Through integrating different datasets from Gene Expression Omnibus (GEO) database, we analyzed merged expression matrix pulpitis, aiming identified biological pathways diagnostic...

Abstract Background: Cone-beam computed tomography (CBCT) was employed to study the morphology and curvature of middle mesial canals (MMCs) mandibular first molars (MFMs). Methods: CBCT scanning performed on MFMs 1100 patients. Patients' images that met inclusion criteria were divided into group A (<40 years old) B (≥40 for further study. The used incidence MMCs at different ages, measure in mesiodistal buccolingual directions using Schneider method, observe anatomical root canal system....

Abstract Background: Cone-beam computed tomography (CBCT) was employed to study the morphology and curvature of middle mesial canals (MMCs) mandibular first molars (MFMs). Methods: CBCT scanning performed on MFMs 1100 patients. Patients' images that met inclusion criteria were divided into group A (<40 years old) B (≥40 for further study. The used incidence MMCs at different ages, measure in mesiodistal buccolingual directions using Schneider method, observe anatomical root canal system....

Abstract Background: Pulpitis is an inflammatory disease, the grade of which classified according to level inflammation. Traditional methods evaluating status dental pulp tissue in clinical practice have limitations. The rapid and accurate diagnosis pulpitis essential for determining appropriate treatment. By integrating different datasets from Gene Expression Omnibus (GEO) database, we analysed a merged expression matrix pulpitis, aiming identify biological pathways diagnostic biomarkers...