A5101738622- Orthopedic Infections and Treatments
- Mesenchymal stem cell research
- Osteoarthritis Treatment and Mechanisms
- Periodontal Regeneration and Treatments
- Orthopaedic implants and arthroplasty
- Total Knee Arthroplasty Outcomes
- Cancer-related molecular mechanisms research
- Bone Tissue Engineering Materials
- TGF-β signaling in diseases
- Lower Extremity Biomechanics and Pathologies
- RNA Interference and Gene Delivery
- Renal Diseases and Glomerulopathies
- dental development and anomalies
- Foot and Ankle Surgery
- Silk-based biomaterials and applications
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Bone and Dental Protein Studies
- NF-κB Signaling Pathways
- Diabetic Foot Ulcer Assessment and Management
- RNA modifications and cancer
- Signaling Pathways in Disease
- Graphene and Nanomaterials Applications
- Antimicrobial Resistance in Staphylococcus
- Bioactive natural compounds
Chongqing Medical University
2016-2025
Soochow University
2025
First Affiliated Hospital of Soochow University
2025
Southern Medical University
2020-2025
Nanfang Hospital
2020-2025
The Affiliated Yongchuan Hospital of Chongqing Medical University
2016-2025
Southern Medical University Shenzhen Hospital
2025
Stomatology Hospital
2025
Chongqing Municipal Health Commission
2024
Beijing University of Chinese Medicine
2024
Background/Aims: Mesenchymal stem cells (MSCs) are multipotent progenitors that can differentiate into several lineages including bone. Successful bone formation requires osteogenesis and angiogenesis coupling of MSCs. Here, we investigate if simultaneous activation BMP9 Notch signaling yields effective osteogenesis-angiogenesis in Methods: Recently-characterized immortalized mouse adipose-derived (iMADs) were used as MSC source. Transgenes BMP9, NICD dnNotch1 expressed by adenoviral...
Introducing hydration layers to hydrogel microspheres (HMs) by coating the surface with liposomes can effectively reduce friction. However, lubrication be inactivated when coatings are damaged. To endow HMs ability form self-renewable and maintain cellular homeostasis, rapamycin-liposome-incorporating hyaluronic acid-based (RAPA@Lipo@HMs) were created using microfluidic technology photopolymerization processes. The RAPA@Lipo@HMs improve joint a smooth rolling mechanism continuously exposing...
Osteomyelitis is a devastating disease caused by microbial infection in deep bone tissue. Its high recurrence rate and impaired restoration of deficiencies are major challenges treatment. Microbes have evolved numerous mechanisms to effectively evade host intrinsic adaptive immune attacks persistently localize the host, such as drug-resistant bacteria, biofilms, persister cells, intracellular small colony variants (SCVs). Moreover, microbial-mediated dysregulation microenvironment impedes...
Mesenchymal stem cells (MSCs) are multipotent progenitor that can undergo self-renewal and differentiate into multiple lineages. Osteogenic differentiation from MSCs is a well-orchestrated process regulated by signaling pathways. We previously demonstrated BMP9 one of the most potent osteogenic factors. However, molecular mechanism through which governs osteoblastic remains to be fully understood. Increasing evidence indicates noncoding RNAs (ncRNAs) may play important regulatory roles in...
Bone morphogenetic protein 2 (BMP2) is one of the key chondrogenic growth factors involved in cartilage regeneration. However, it also exhibits osteogenic abilities and triggers endochondral ossification. Effective chondrogenesis inhibition BMP2-induced osteogenesis ossification can be achieved by directing mesenchymal stem cells (MSCs) towards chondrocyte lineage with chodrogenic factors, such as Sox9. Here we investigated effects Sox9 on differentiation MSCs. We found exogenous...
The liver provides vital metabolic, exocrine and endocrine functions in the body as such pathological conditions of lead to high morbidity mortality. is highly regenerative contains facultative stem cells that become activated during injury replicate fully recover mass function. Canonical Wnt/β-catenin signaling plays an important role regulating proliferation differentiation progenitor regeneration. However, possible roles noncanonical Wnts development regeneration remain undefined. We...
Mesenchymal stem cells (MSCs) are multipotent progenitors, which can undergo self-renewal and give rise to multi-lineages. A great deal of attentions have been paid their potential use in regenerative medicine as therapeutic genes be introduced into MSCs. Genetic manipulations MSCs requires effective gene deliveries. Recombinant adenoviruses widely used transfer vectors. We found that although infected vitro by adenoviruses, high virus titers needed achieve efficiency. Here, we investigate...
Background/Aims: Joint cartilage defects are difficult to treat due the limited self-repair capacities of cartilage. Cartilage tissue engineering based on stem cells and gene enhancement is a potential alternative for repair. Bone morphogenetic protein 2 (BMP2) has been shown induce chondrogenic differentiation in mesenchymal (MSCs); however, maintaining phenotypes MSCs during repair since occurs along endochondral ossification pathway. In this study, hypoxia inducible factor, or (HIF)-1α,...
Mesenchymal stem cells (MSCs) are multipotent non-hematopoietic progenitor that can undergo self-renewal and differentiate into multi-lineages. Bone marrow stromal (BMSCs) represent one of the most commonly-used MSCs. In order to overcome technical challenge maintaining primary BMSCs in long-term culture, here we seek establish reversibly immortalized mouse (imBMSCs). By exploiting CRISPR/Cas9-based homology-directed-repair (HDR) mechanism, target SV40T Rosa26 locus efficiently immortalize...
The treatment options for diabetic retinopathy (DR) are limited. Mesenchymal stem cells (MSCs) a promising option diabetes and its complications. In this pilot clinical trial, we evaluated the safety efficacy of intravenous autologous bone marrow MSCs (ABMSC) DR.In total, 34 eyes with non-proliferative or proliferative DR (NPDR, n = 19; PDR, 15) from 17 patients were analyzed. Treatment involved one infusion 3 × 106/kg ABSMCs. patients' vital signs monitored, along immune allergic reactions....
Effective bone tissue engineering is important to overcome the unmet clinical challenges as more than 1.6 million grafts are done annually in United States. Successful needs minimally three critical constituents: osteoprogenitor cells, osteogenic factors, and osteoinductive/osteoconductive scaffolds. Osteogenic progenitors derived from multipotent mesenchymal stem cells (MSCs), which can be prepared numerous sources, including adipose tissue. We previously showed that BMP9 most BMP induces...
Diagnosing chronic periprosthetic joint infection (PJI) is challenging. Synovial fluid interleukin-10 (SF IL-10), an anti-inflammatory cytokine produced by leukocytes, plays a pivotal role in inflammation and regulation. However, limited research has explored the diagnostic potential of SF IL-10 PJI patients. The study aimed to investigate relationship between incidence PIJ, evaluate its reliability. We analyzed data from 137 patients who underwent revision surgery for aseptic loosening or...
The crosstalk between megakaryocytic lineage cells and the skeletal system has just begun to be explored but remains largely elusive. Using conditional gene knockout mouse models, we demonstrated that loss of Beclin 1 (Becn1), a major regulator mammalian autophagy, exclusively in disrupted autophagy platelets did not compromise megakaryopoiesis or formation function platelets. Unexpectedly, Becn1 deletion male mice led remarkable increase bone mass with improved quality, association decrease...
Alternative splicing not only expands the genetic encoding of genes but also determines cellular activities. This study aimed to elucidate regulation mechanism and biological functions lincRNA-ASAO in process odontogenesis-related alternative mediated odontogenic differentiation hDPSCs. RACE, RNA-seq, FISH bioinformatics techniques were used identify novel lincRNA-ASAO. ALP staining, alizarin red qRT-PCR western blot role regulating odontoblast The binding protein PTBP1 was screened by...
Abstract The cranial suture complex is a heterogeneous tissue consisting of osteogenic progenitor cells and mesenchymal stem ( MSC s) from bone marrow mesenchyme. fusion sutures highly coordinated tightly regulated process during development. Craniosynostosis congenital malformation caused by premature sutures. While the derived should prove valuable for studying molecular mechanisms underlying development pathogenic fusion, primary human progenitors (SuPs) have limited life span gradually...
Sustained, high level transgene expression in mammalian cells is desired many cases for studying gene functions. Traditionally, stable has been accomplished by using retroviral or lentiviral vectors. However, such viral vector-mediated often at low levels and can be reduced over time due to copy numbers and/or chromatin remodeling repression. The piggyBac transposon emerged as a promising non-viral vector system efficient transfer into cells. Despite its inherent advantages systems, not...
Cartilage injuries caused by arthritis or trauma pose formidable challenges for effective clinical management due to the limited intrinsic proliferative capability of chondrocytes. Autologous stem cell-based therapies and transgene-enhanced cartilage tissue engineering may open new avenues treatment injuries. Bone morphogenetic protein 2 (BMP2) induces chondrogenesis mesenchymal cells (MSCs) can thus be explored as a potential therapeutic agent defect repair. However, BMP2 also robust...
Lev/MSNs/n-HA/PU has been proved to be a novel scaffold material treat bone defect caused by chronic osteomyelitis. We have previously identified that this can effectively osteomyelitis Staphylococcus aureus in vivo. However, the potential mechanisms of antibacterial and osteogenic induction properties remain unclear. Thus, for osteogenesis property, immunohistochemistry, PCR, Western blot were performed detect expression markers. Furthermore, flow cytometry TUNEL applied analyze MC3T3-E1...
Tooth development is regulated by sequential and reciprocal epithelium-mesenchymal interactions their related molecular signaling pathways, such as bone morphogenetic proteins (BMPs). Among the 14 types of BMPs, BMP9 (also known growth differentiation factor 2) one most potent BMPs to induce osteogenic mesenchymal stem cells. The purpose this study was examine potential roles in tooth development. First, we detected expression pattern germ during postnatal development, found that widely...
Abstract Background Bone morphogenetic protein 2 (BMP2) is a promising chondrogenic growth factor for cartilage tissue-engineering, but it also induces robust endochondral ossification. Human synovial-derived mesenchymal stromal cells (hSMSCs) have attracted great interest due to their poor potential differentiation into osteogenic lineages. Smad7 plays significant in the In this study, we explored new method amplify BMP2-induced of hSMSCs by downregulating and applying cellular scaffold....
Macrophages (Mφs) play a crucial role in the pathological progression of osteoarthritis (OA) by regulating inflammation and tissue repair. Decreasing pro-inflammatory M1-Mφs increasing anti-inflammatory M2-Mφs can alleviate OA-related promote cartilage Apoptosis is natural process associated with A large number apoptotic bodies (ABs), type extracellular vesicle, are produced during apoptosis, this reduction inflammation. However, functions remain largely unknown. In study, we investigated...
Retroviral vectors including lentiviral are commonly used tools to stably express transgenes or RNA molecules in mammalian cells. Their utilities roughly divided into two categories, stable overexpression of and molecules, which requires maximal transduction efficiency, functional selection with retrovirus (RV)-based libraries, takes advantage retroviral superinfection resistance. However, the dynamic features RV-mediated not well characterized. Here, we engineered murine stem cell...