A5058661277- TGF-β signaling in diseases
- Mesenchymal stem cell research
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Connective Tissue Growth Factor Research
- Tissue Engineering and Regenerative Medicine
- Sarcoma Diagnosis and Treatment
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Bone Tissue Engineering Materials
- MicroRNA in disease regulation
- Growth Hormone and Insulin-like Growth Factors
- Peroxisome Proliferator-Activated Receptors
- Periodontal Regeneration and Treatments
- Electrospun Nanofibers in Biomedical Applications
- RNA Research and Splicing
- Bone Metabolism and Diseases
- Bone and Dental Protein Studies
- CRISPR and Genetic Engineering
- Orthopedic Infections and Treatments
- Cancer Cells and Metastasis
- Orthopaedic implants and arthroplasty
- Hip disorders and treatments
University of Chicago Medical Center
2016-2025
Molecular Oncology (United States)
2016-2025
University of Chicago
2010-2024
Stryker (United States)
2020
Hội Gastroenterology Việt Nam
2020
Brigham and Women's Hospital
2020
Harvard University
2020
Chongqing Medical University
2008-2010
Daping Hospital
2010
Army Medical University
2010
Background/Aims: Mesenchymal stem cells (MSCs) are multipotent progenitors that can differentiate into several lineages including bone. Successful bone formation requires osteogenesis and angiogenesis coupling of MSCs. Here, we investigate if simultaneous activation BMP9 Notch signaling yields effective osteogenesis-angiogenesis in Methods: Recently-characterized immortalized mouse adipose-derived (iMADs) were used as MSC source. Transgenes BMP9, NICD dnNotch1 expressed by adenoviral...
Background: Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials currently underway evaluate the ability of certain BMPs fracture-healing spinal fusion. The optimal be used in different applications have not been elucidated, a comprehensive evaluation relative osteogenic activity is lacking. Methods: To identify that may possess most osteoinductive activity, we analyzed mesenchymal progenitor osteoblastic cells. Recombinant adenoviruses expressing fourteen...
Advances in three-dimensional (3D) printing have increased feasibility towards the synthesis of living tissues. Known as 3D bioprinting, this technology involves precise layering cells, biologic scaffolds, and growth factors with goal creating bioidentical tissue for a variety uses. Early successes demonstrated distinct advantages over conventional engineering strategies. Not surprisingly, there are current challenges to address before bioprinting becomes clinically relevant. Here we provide...
Abstract Efficacious bone regeneration could revolutionize the clinical management of many and musculoskeletal disorders. Bone morphogenetic proteins (BMPs) can regulate differentiation mesenchymal stem cells into cartilage, bone, tendon/ligament, fat lineages. Early data documented osteogenic potential rhBMP2 rhBMP7/OP‐1. However, prior to this work that summarized several our recent studies, no comprehensive analysis had been undertaken characterize relative activity all BMPs. Using...
Pluripotent mesenchymal stem cells (MSCs) are bone marrow stromal progenitor that can differentiate into osteogenic, chondrogenic, adipogenic, and myogenic lineages. Several signaling pathways have been shown to regulate the lineage commitment terminal differentiation of MSCs. Here, we conducted a comprehensive analysis 14 types morphogenetic protein (BMPs) for their abilities multilineage specific We found most BMPs exhibited distinct expression Runx2, Sox9, MyoD, PPARgamma2. Further...
The Wnt signaling pathway plays an important role not only in embryonic development but also the maintenance and differentiation of stem cells adulthood. In particular, has been shown as regulatory osteogenic mesenchymal cells. Induction promotes bone formation while inactivation leads to osteopenic states. Our current understanding osteogenesis elucidates molecular mechanisms classic pathologies. Activating inactivating aberrations canonical results sclerosteosis osteoporosis respectively....
One of the greatest obstacles to current cancer treatment efforts is development drug resistance by tumors. Despite recent advances in diagnostic practices and surgical interventions, many neoplasms demonstrate poor response adjuvant or neoadjuvant radiation chemotherapy. As a result, prognosis for patients afflicted with these aggressive cancers remains bleak. The insulin-like growth factor (IGF) signaling axis has been shown play critical role progression various Many basic science...
Bone morphogenetic proteins (BMPs) are members of the TGF-β superfamily and have diverse functions during development organogenesis. BMPs play a major role in skeletal bone formation, disruptions BMP signaling cause variety extraskeletal anomalies. Several knockout models provided insight into mechanisms responsible for these phenotypes. Proper formation requires differentiation osteoblasts from mesenchymal stem cell (MSC) precursors, process mediated part by signaling. Multiple BMPs,...
The canonical WNT/β-catenin signaling pathway governs a myriad of biological processes underlying the development and maintenance adult tissue homeostasis, including regulation stem cell self-renewal, proliferation, differentiation, apoptosis. WNTs are secreted lipid-modified glycoproteins that act as short-range ligands to activate receptor-mediated pathways. hallmark is activation β-catenin-mediated transcriptional activity. Canonical control β-catenin dynamics cytoplasmic level tightly...
Effective bone regeneration through tissue engineering requires a combination of osteogenic progenitors, osteoinductive biofactors and biocompatible scaffold materials. Mesenchymal stem cells (MSCs) represent the most promising seed for engineering. As multipotent that can self-renew differentiate into multiple lineages including fat, MSCs be isolated from numerous tissues exhibit varied differentiation potential. To identify an optimal progenitor cell source engineering, we analyzed...
Osteoblast lineage-specific differentiation of mesenchymal stem cells is a well regulated but poorly understood process. Both bone morphogenetic proteins (BMPs) and Wnt signaling are implicated in regulating osteoblast formation. Here we analyzed the expression profiles stimulated with Wnt3A osteogenic BMPs, identified connective tissue growth factor (CTGF) as potential target BMP signaling. We confirmed microarray results, demonstrated that CTGF was up-regulated at early stage BMP-9...
Marrow mesenchymal stem cells are pluripotent progenitors that can differentiate into bone, cartilage, muscle, and fat cells. Wnt signaling has been implicated in regulating osteogenic differentiation of Here, we analyzed the gene expression profile were stimulated with Wnt3A. Among 220 genes whose was significantly changed by 2.5-fold, found three members CCN family, CCN1/Cyr61, CCN2/connective tissue growth factor (CTGF), CCN5/WISP2, among most up-regulated genes. We further investigated...
Abstract Efficient osteogenic differentiation and bone formation from mesenchymal stem cells (MSCs) should have clinical applications in treating nonunion fracture healing. MSCs are adherent marrow stromal that can self-renew differentiate into osteogenic, chondrogenic, adipogenic, myogenic lineages. We identified morphogenetic protein 9 (BMP-9) as one of the most BMPs. Here we investigate effect insulin-like growth factor 2 (IGF-2) on BMP-9-induced formation. found endogenous IGF-2...
Bone morphogenetic proteins (BMPs) belong to the TGF-β superfamily and play an important role in development many cellular processes. We have found that BMP-2, BMP-6, BMP-9 induce most potent osteogenic differentiation of mesenchymal stem cells. Expression profiling analysis has revealed Inhibitors DNA binding/differentiation (Id)-1, Id-2, Id-3 are among significantly up-regulated genes upon or stimulation. Here, we sought determine functional these Id BMP-induced osteoblast differentiation....
Bone morphogenetic protein 9 (BMP-9) is a member of the transforming growth factor (TGF)-beta/BMP superfamily, and we have demonstrated that it one most potent BMPs to induce osteoblast differentiation mesenchymal stem cells (MSCs). Here, sought investigate if canonical Wnt/beta-catenin signalling plays an important role in BMP-9-induced osteogenic MSCs. Wnt3A BMP-9 enhanced each other's ability alkaline phosphatase (ALP) MSCs mouse embryonic fibroblasts (MEFs). Wnt antagonist FrzB was shown...
Mesenchymal stem cells (MSCs) are non-hematopoietic with the capacity to differentiate into tissues of both mesenchymal and non-mesenchymal origin.MSCs can osteoblastic, chondrogenic, adipogenic lineages, although recent studies have demonstrated that MSCs also able other including neuronal cardiomyogenic lineages.Since their original isolation from bone marrow, been successfully harvested many tissues.Their ease ex vivo expansion combined immunoprivileged nature has made these popular...