A5008026933- TGF-β signaling in diseases
- Bone Metabolism and Diseases
- Mesenchymal stem cell research
- Bone and Dental Protein Studies
- Growth Hormone and Insulin-like Growth Factors
- Wnt/β-catenin signaling in development and cancer
- Retinoids in leukemia and cellular processes
- Connective Tissue Growth Factor Research
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Peroxisome Proliferator-Activated Receptors
- Cancer-related molecular mechanisms research
- Berberine and alkaloids research
- Cancer, Hypoxia, and Metabolism
- Aluminum toxicity and tolerance in plants and animals
- Quinazolinone synthesis and applications
- PI3K/AKT/mTOR signaling in cancer
- Bioactive Natural Diterpenoids Research
- Tissue Engineering and Regenerative Medicine
- Heterotopic Ossification and Related Conditions
- Sirtuins and Resveratrol in Medicine
- Medicinal Plants and Neuroprotection
- Cancer Mechanisms and Therapy
- Synthesis and Biological Evaluation
- Kruppel-like factors research
Chongqing Medical University
2016-2025
University of Chicago Medical Center
2008-2023
Molecular Oncology (United States)
2008-2023
Chongqing Medical and Pharmaceutical College
2013-2023
State Council of the People's Republic of China
2019-2021
The Affiliated Yongchuan Hospital of Chongqing Medical University
2017
Ministry of Education of the People's Republic of China
2012
Children's Hospital of Chongqing Medical University
2008-2010
Daping Hospital
2010
Sichuan University
2010
Pluripotent mesenchymal stem cells (MSCs) are bone marrow stromal progenitor that can differentiate into osteogenic, chondrogenic, adipogenic, and myogenic lineages. Several signaling pathways have been shown to regulate the lineage commitment terminal differentiation of MSCs. Here, we conducted a comprehensive analysis 14 types morphogenetic protein (BMPs) for their abilities multilineage specific We found most BMPs exhibited distinct expression Runx2, Sox9, MyoD, PPARgamma2. Further...
Abstract Efficient osteogenic differentiation and bone formation from mesenchymal stem cells (MSCs) should have clinical applications in treating nonunion fracture healing. MSCs are adherent marrow stromal that can self-renew differentiate into osteogenic, chondrogenic, adipogenic, myogenic lineages. We identified morphogenetic protein 9 (BMP-9) as one of the most BMPs. Here we investigate effect insulin-like growth factor 2 (IGF-2) on BMP-9-induced formation. found endogenous IGF-2...
Bone morphogenetic protein 9 (BMP-9) is a member of the transforming growth factor (TGF)-beta/BMP superfamily, and we have demonstrated that it one most potent BMPs to induce osteoblast differentiation mesenchymal stem cells (MSCs). Here, sought investigate if canonical Wnt/beta-catenin signalling plays an important role in BMP-9-induced osteogenic MSCs. Wnt3A BMP-9 enhanced each other's ability alkaline phosphatase (ALP) MSCs mouse embryonic fibroblasts (MEFs). Wnt antagonist FrzB was shown...
Mesenchymal stem cells (MSCs) are non-hematopoietic with the capacity to differentiate into tissues of both mesenchymal and non-mesenchymal origin.MSCs can osteoblastic, chondrogenic, adipogenic lineages, although recent studies have demonstrated that MSCs also able other including neuronal cardiomyogenic lineages.Since their original isolation from bone marrow, been successfully harvested many tissues.Their ease ex vivo expansion combined immunoprivileged nature has made these popular...
Pluripotent mesenchymal stem cells (MSCs) are bone marrow stromal progenitor that can differentiate into osteogenic, chondrogenic, adipogenic, and myogenic lineages. We previously demonstrated morphogenetic protein (BMP) 9 is one of the most potent yet least characterized BMPs able to induce osteogenic differentiation MSCs both in vitro vivo. Here, we conducted gene expression-profiling analysis identified Hey1 hairy/Enhancer split-related repressor basic helix-loop-helix family was among...
Mesenchymal stem cells (MSCs) are bone marrow stromal that can differentiate into multiple lineages. We previously demonstrated BMP9 is one of the most potent BMPs to induce osteogenic differentiation MSCs. least studied BMPs. Whereas ALK1, ALK5, and/or endoglin have recently been reported as potential type I receptors in endothelial cells, little known about receptor involvement BMP9-induced Here, we conduct a comprehensive analysis functional role seven signaling found expressed However,...
Promoting osteogenic differentiation and efficacious bone regeneration have the potential to revolutionize treatment of orthopaedic musculoskeletal disorders. Mesenchymal Stem Cells (MSCs) are marrow progenitor cells that capacity differentiate along osteogenic, chondrogenic, myogenic, adipogenic lineages. Differentiation these lineages is a tightly controlled process in part regulated by Bone Morphogenetic Proteins (BMPs). BMPs 2 7 been approved for clinical use because their osteoinductive...
As one of the most common malignancies, colon cancer is initiated by abnormal activation Wnt/β-catenin pathway. Although treatment options have increased for some patients, overall progress has been modest. Thus, there a great need to develop new treatments. We found that bisbenzylisoquinoline alkaloid tetrandrine (TET) exhibits anticancer activity. TET used as calcium channel blocker treat hypertensive and arrhythmic conditions in Chinese medicine. Here, we investigate molecular basis...
Background As one of the least studied bone morphogenetic proteins (BMPs), BMP9 is most osteogenic BMPs. Retinoic acid (RA) signaling known to play an important role in development, differentiation and metabolism. In this study, we investigate effect RA on BMP9-induced mesenchymal progenitor cells (MPCs). Methodology/Principal Findings Both primary MPCs MPC line are used for stimulation. Recombinant adenoviruses deliver BMP9, RARα RXRα into MPCs. The vitro monitored by determining early late...
Mesenchymal stem cells (MSCs) are multipotent progenitor that can undergo self-renewal and differentiate into multiple lineages. Osteogenic differentiation from MSCs is a well-orchestrated process regulated by signaling pathways. We previously demonstrated BMP9 one of the most potent osteogenic factors. However, molecular mechanism through which governs osteoblastic remains to be fully understood. Increasing evidence indicates noncoding RNAs (ncRNAs) may play important regulatory roles in...
Abstract Growth hormone (GH) is usually released by somatotrophs in the anterior pituitary response to GH-releasing and plays an important role skeleton development postnatal growth. However, it unclear if extrapituitary GH exerts any effect on murine multilineage cells (MMCs). MMCs are multipotent progenitors that give rise several lineages, including bone, cartilage, fat. We have identified bone morphogenic protein 9 (BMP9) as one of most osteogenic BMPs regulating a distinct set...
Colon cancer is one of the most common malignancies and treatments for colon have been developed substantially in last decades, but there still a great clinical need to explore new treatment regimens due undesirable prognosis. In this investigation, we demonstrated anti-proliferative apoptosis-inducing activities resveratrol (Res) human cells, possible mechanisms underlying these effects. We used crystal violet staining, flow cytometry western blotting validate effects Res on HCT116 cells. A...
Osteosarcoma is the most common primary malignancy of bone. The long-term survival osteosarcoma patients hinges on our ability to prevent and/or treat recurrent and metastatic lesions. Here, we investigated activation peroxisome proliferator-activated receptor gamma (PPARgamma) retinoid receptors as a means differentiation therapy for human osteosarcoma.We examined endogenous expression PPARgamma in panel cells. Ligands or adenovirus-mediated overexpression these were tested inhibit...
Mesenchymal stem cells (MSCs) are multipotent which reside in many tissues and can give rise to multiple lineages including bone, cartilage adipose. Although MSCs have attracted significant attention for basic translational research, primary limited life span culture hampers MSCs' broader applications. Here, we investigate if mouse mesenchymal progenitors be conditionally immortalized with SV40 large T antigen maintain long-term cell proliferation without compromising their multipotency....
Osteosarcoma (OS) is associated with poor prognosis due to its high incidence of metastasis and chemoresistance. It often arises in areas rapid bone growth long bones during the adolescent spurt. Although certain genetic conditions alterations increase risk developing OS, molecular pathogenesis poorly understood. Recently, defects differentiation have been linked cancers, as they are cell proliferation. Treatments overcoming these enable terminal subsequent tumor inhibition. OS development...
Notch is an important pathway in that it regulates cell-to-cell signal transduction, which plays essential role skeletal remodeling. Bone morphogenetic protein (BMP)9 has been regarded as one of the most efficient BMPs by to induce osteogenic differentiation mesenchymal stem cells (MSCs). Understanding interaction between and BMP9 signaling a critical issue for optimizing application MSCs bone tissue engineering. In present study, we investigated BMP9‑induced MSCs. Our data demonstrated...
Osteoporosis is a common systemic bone disease caused by the imbalance between osteogenic activity and osteoclastic activity. Aged women are at higher risk of osteoporosis, partly because estrogen deficiency. However, underlying mechanism how deficiency affects osteoclast has not yet been well elucidated. In this study, GSE2208 GSE56815 datasets were downloaded from GEO database with 25 PreH BMD PostL in total. The RRA algorithm determined 38 downregulated DEGs 30 upregulated DEGs. Through...
Abstract Wnt/β‐catenin pathway plays an important role in regulating embryonic development. Hepatocytes differentiate from endoderm during Hepatic progenitor cells (HPCs) have been isolated fetal liver and extrahepatic tissues. Most current studies development hepatic differentiation focused on Wnts, β‐catenin, their receptors. Here, we sought to determine the of Wnt antagonists liver‐derived HPCs. Using mouse tissues derived day E12.5 postnatal (PD) 28, found that 13 19 genes almost all...
Hepatic progenitor cells (HPCs) can be isolated from fetal liver and extrahepatic tissues. Retinoic acid (RA) signalling plays an important role in development, although the of RA liver-specific progenitors is poorly understood.We sought to determine regulating hepatic differentiation.RNA was tissues various developmental stages. Liver marker expression assessed by reverse transcriptase-polymerase chain reaction immunofluorescence staining. Reversibly immortalized HPCs derived mouse...