A5017906802- Lipid metabolism and disorders
- Diabetes, Cardiovascular Risks, and Lipoproteins
- TGF-β signaling in diseases
- Caveolin-1 and cellular processes
- Drug Transport and Resistance Mechanisms
- Connective Tissue Growth Factor Research
- Wnt/β-catenin signaling in development and cancer
- Immune cells in cancer
- Adipose Tissue and Metabolism
- Immune Cell Function and Interaction
- Cholesterol and Lipid Metabolism
- Animal Nutrition and Physiology
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
- Diabetes and associated disorders
- Retinoids in leukemia and cellular processes
- Animal Behavior and Welfare Studies
- MicroRNA in disease regulation
- Lipid metabolism and biosynthesis
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Endoplasmic Reticulum Stress and Disease
- Ginseng Biological Effects and Applications
- Cancer-related molecular mechanisms research
- Liver Disease Diagnosis and Treatment
University of California, Los Angeles
2020-2024
The First Affiliated Hospital, Sun Yat-sen University
2024
Sun Yat-sen University
2024
Hunan Normal University
2022-2023
Tsinghua University
2016-2022
Capital Medical University
2019
Xinxiang Medical University
2018
Children's Hospital of Chongqing Medical University
2008
Chongqing Medical University
2008
University of Chicago Medical Center
2006-2008
Abstract Efficacious bone regeneration could revolutionize the clinical management of many and musculoskeletal disorders. Bone morphogenetic proteins (BMPs) can regulate differentiation mesenchymal stem cells into cartilage, bone, tendon/ligament, fat lineages. Early data documented osteogenic potential rhBMP2 rhBMP7/OP‐1. However, prior to this work that summarized several our recent studies, no comprehensive analysis had been undertaken characterize relative activity all BMPs. Using...
Pluripotent mesenchymal stem cells (MSCs) are bone marrow stromal progenitor that can differentiate into osteogenic, chondrogenic, adipogenic, and myogenic lineages. Several signaling pathways have been shown to regulate the lineage commitment terminal differentiation of MSCs. Here, we conducted a comprehensive analysis 14 types morphogenetic protein (BMPs) for their abilities multilineage specific We found most BMPs exhibited distinct expression Runx2, Sox9, MyoD, PPARgamma2. Further...
Marrow mesenchymal stem cells are pluripotent progenitors that can differentiate into bone, cartilage, muscle, and fat cells. Wnt signaling has been implicated in regulating osteogenic differentiation of Here, we analyzed the gene expression profile were stimulated with Wnt3A. Among 220 genes whose was significantly changed by 2.5-fold, found three members CCN family, CCN1/Cyr61, CCN2/connective tissue growth factor (CTGF), CCN5/WISP2, among most up-regulated genes. We further investigated...
Bone morphogenetic protein 9 (BMP-9) is a member of the transforming growth factor (TGF)-beta/BMP superfamily, and we have demonstrated that it one most potent BMPs to induce osteoblast differentiation mesenchymal stem cells (MSCs). Here, sought investigate if canonical Wnt/beta-catenin signalling plays an important role in BMP-9-induced osteogenic MSCs. Wnt3A BMP-9 enhanced each other's ability alkaline phosphatase (ALP) MSCs mouse embryonic fibroblasts (MEFs). Wnt antagonist FrzB was shown...
Pluripotent mesenchymal stem cells (MSCs) are bone marrow stromal progenitor that can differentiate into osteogenic, chondrogenic, adipogenic, and myogenic lineages. We previously demonstrated morphogenetic protein (BMP) 9 is one of the most potent yet least characterized BMPs able to induce osteogenic differentiation MSCs both in vitro vivo. Here, we conducted gene expression-profiling analysis identified Hey1 hairy/Enhancer split-related repressor basic helix-loop-helix family was among...
Intestinal absorption is an important contributor to systemic cholesterol homeostasis. Niemann-Pick C1 Like 1 (NPC1L1) assists in the initial step of dietary uptake, but how moves downstream NPC1L1 unknown. We show that Aster-B and Aster-C are critical for nonvesicular movement enterocytes. Loss diminishes accessible plasma membrane (PM) abolishes Aster recruitment intestinal brush border. Enterocytes lacking Asters accumulate PM endoplasmic reticulum depletion. Aster-deficient mice have...
Abstract Nucleic acid therapeutics (NATs) have proven useful in promoting the degradation of specific transcripts, modifying gene expression, and regulating mRNA splicing. In each situation, efficient delivery nucleic acids to cells, tissues intracellular compartments is crucial—both for optimizing efficacy reducing side effects. Despite successes NATs, our understanding their cellular uptake distribution limited. Current methods yielded insights into NATs within cells tissues, but...
American ginseng (Panax quinquefolius L., Araliaceae) possesses anti-cancer potential and is one of the most commonly used herbal medicines in United States. Ginsenoside Rg3, saponins ginseng, has been shown to inhibit tumor growth. In this study, we sought characterize downstream genes targeted by extracts HCT-116 human colorectal cancer cells. We first demonstrated that content Rg3 steamed at 120°C for 2 h (referred as S2h) was significantly increased when compared with unsteamed ginseng....
The human Glucose Transporter 1 (hGLUT1 or SLC2A1) is a facilitative membrane transporter found in the liver, intestines, kidney, and brain, where it transports sugars such as d-glucose d-galactose. Genetic variations hGLUT1 are associated with broad range of diseases metabolic disorders. For example, upregulated various cancer types (e.g., breast carcinoma) to support increased anaerobic glycolysis Warburg effect. Thus, an emerging therapeutic target, which also commonly used biomarkers...
Beiging of white adipose tissue (WAT) is a particularly appealing target for therapeutics in the treatment metabolic diseases through norepinephrine (NE)-mediated signaling pathways. Although previous studies report NE clearance mechanisms via SLC6A2 on sympathetic neurons or proinflammatory macrophages tissues (ATs), low catecholamine capacity may limit cleaning efficiency. Here, we that mouse organic cation transporter 3 (Oct3; Slc22a3) highly expressed WAT and displays greatest uptake...
GPIHBP1, an endothelial cell (EC) protein, captures lipoprotein lipase (LPL) within the interstitial spaces (where it is secreted by myocytes and adipocytes) transports across ECs to its site of action in capillary lumen. GPIHBP1's 3-fingered LU domain required for LPL binding, but function acidic (AD) has remained unclear. We created mutant mice lacking AD found severe hypertriglyceridemia. As expected, GPIHBP1 retained capacity bind LPL. Unexpectedly, however, most was located on abluminal...
Lipoprotein lipase (LPL), the enzyme that carries out lipolytic processing of triglyceride-rich lipoproteins (TRLs), is synthesized by adipocytes and myocytes secreted into interstitial spaces. The LPL then bound GPIHBP1, a GPI-anchored protein endothelial cells (ECs), transported across ECs to capillary lumen. assumption has been moved capillaries remains attached GPIHBP1 serves as platform for TRL processing. In current studies, we examined validity assumption. We found an LPL-specific...
Abstract Despite the therapeutic success of tenofovir (TFV) for treatment HIV-1 infection, numerous cases nephrotoxicity have been reported. Mitochondrial toxicity has purported as major target TFV-associated renal tubulopathy but underlying molecular mechanism remains unclear. In this report, we use metabolomics and proteomics with HK-2 cells animal models to dissect pathways nephropathy caused by TFV its more toxic analog, adefovir (ADV). Proteomic analysis shows that mitochondrial...
Bromine and peroxidasin (an extracellular peroxidase) are essential for generating sulfilimine cross-links between a methionine hydroxylysine within collagen IV, basement membrane protein. The increase the structural integrity of membranes. formation depends on ability to use bromide hydrogen peroxide substrates produce hypobromous acid (HOBr). Once cross-link is created, released into space becomes available reutilization. Whether HOBr generated by used very selectively creating or whether...
Cholesterol-laden macrophage foam cells are a hallmark of atherosclerosis. For that reason, cholesterol metabolism in macrophages has attracted considerable scrutiny, particularly the mechanisms by which unload surplus (a process referred to as "cholesterol efflux"). Many studies efflux have focused on role ABC transporters moving onto high-density lipoproteins (HDLs), but other for likely exist. We hypothesized capacity directly adjacent cells. To test this hypothesis, we used...
GPIHBP1, a protein of capillary endothelial cells (ECs), is crucial partner for lipoprotein lipase (LPL) in the lipolytic processing triglyceride-rich lipoproteins. which contains three-fingered cysteine-rich LU (Ly6/uPAR) domain and an intrinsically disordered acidic (AD), captures LPL from within interstitial spaces (where it secreted by parenchymal cells) shuttles across ECs to lumen. Without remains stranded spaces, causing severe hypertriglyceridemia (chylomicronemia). Biophysical...
Highlights•Loss of hepatic SEL1L-HRD1 ERAD causes hyperproliferation and propensity to cancer•Proteomics SEL1L-deficient microsomes reveal WNT5A as a potential link•WNT5A is misfolding prone an endogenous substrate in the liver•Hepatic levels correlate with better prognosis patients liver cancerSummaryEndoplasmic reticulum (ER) homeostasis has been implicated pathogenesis various forms cancer; however, our understanding role ER quality control mechanisms tumorigenesis remains incomplete....
Why apolipoprotein AV (APOA5) deficiency causes hypertriglyceridemia has remained unclear, but we suspected that the underlying cause was reduced amounts of lipoprotein lipase (LPL) in capillaries. By routine immunohistochemistry, observed LPL staining heart and brown adipose tissue (BAT) capillaries Apoa5–/– mice. Also, after an intravenous injection LPL-, CD31-, GPIHBP1-specific monoclonal antibodies, binding antibodies to BAT (relative CD31 or GPIHBP1 antibodies) levels postheparin plasma...