A5048831169- Lipid metabolism and biosynthesis
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Cholesterol and Lipid Metabolism
- Drug Transport and Resistance Mechanisms
- Peptidase Inhibition and Analysis
- Microbial Metabolic Engineering and Bioproduction
- Advanced biosensing and bioanalysis techniques
- Biotin and Related Studies
- HIV Research and Treatment
- Photosynthetic Processes and Mechanisms
- RNA Research and Splicing
- Diabetes Treatment and Management
- Receptor Mechanisms and Signaling
- Click Chemistry and Applications
- Cardiovascular Disease and Adiposity
- Hormonal and reproductive studies
- Peroxisome Proliferator-Activated Receptors
- Lipid Membrane Structure and Behavior
- Growth Hormone and Insulin-like Growth Factors
- Diet and metabolism studies
- Muscle metabolism and nutrition
- Enzyme Catalysis and Immobilization
- Glycosylation and Glycoproteins Research
- Retinoids in leukemia and cellular processes
Institute of Biophysics
2015-2024
Chinese Academy of Sciences
2015-2024
University of California, Los Angeles
2022-2024
University of Science and Technology of China
2015-2021
Lipid droplet (LD), a multi-functional organelle, is often found to associate with other cellular membranous structures and vary in size given cell, which may be related their functional diversity. Here we established method separate LD subpopulations from isolated CHO K2 LDs into three different categories. The subpopulation smallest was nearly free of ER while those larger contained intact ER. These distinct differed protein composition ability recruit proteins. This also applicable...
Intestinal absorption is an important contributor to systemic cholesterol homeostasis. Niemann-Pick C1 Like 1 (NPC1L1) assists in the initial step of dietary uptake, but how moves downstream NPC1L1 unknown. We show that Aster-B and Aster-C are critical for nonvesicular movement enterocytes. Loss diminishes accessible plasma membrane (PM) abolishes Aster recruitment intestinal brush border. Enterocytes lacking Asters accumulate PM endoplasmic reticulum depletion. Aster-deficient mice have...
Aster proteins mediate the nonvesicular transport of cholesterol from plasma membrane (PM) to endoplasmic reticulum (ER). However, importance sterol movement for physiology and pathophysiology in various tissues is incompletely understood. Here we show that loss Aster-B leads diet-induced obesity insulin resistance female but not male mice, this sex difference abolished by ovariectomy. We further demonstrate deficiency impairs PM ER ovaries vivo, leading hypogonadism reduced estradiol...
The lipid droplet (LD)-associated protein adipose differentiation-related (ADRP or PLIN2) is required for the formation and stability of LD organelle, whereas its biological roles are still obscure. Herein, we show that PLIN2 most abundant on droplets (LDs) mouse myoblast cell line C2C12. Both expression accumulation LDs were up-regulated in a time- dose-dependent manner when cells treated with oleate (OA). level was positively correlated LDs, suggesting stabilize PLIN2. Furthermore,...
Ketogenesis requires fatty acid flux from intracellular (lipid droplets) and extrahepatic (adipose tissue) lipid stores to hepatocyte mitochondria. However, whether interorganelle contact sites regulate this process is unknown. Recent studies have revealed a role for Calsyntenin-3β (CLSTN3β), an endoplasmic reticulum–lipid droplet site protein, in the control of utilization adipose tissue. Here, we show that Clstn3b expression induced liver by nuclear receptor PPARα settings high...
The plasma membrane proteome is a rich resource of functionally important and therapeutically relevant protein targets. Distinguished by high hydrophobicity, heavy glycosylation, disulfide-rich sequences, low overall abundance, the cell surface remains undersampled in established proteomic pipelines, including our own cysteine chemoproteomics platforms. Here, we paired glycoprotein capture with to establish two-stage enrichment method that enables chemoproteomic profiling Surface Cysteinome....
It is crucial to understand the glucose control within our bodies. Bariatric/metabolic surgeries, including laparoscopic sleeve gastrectomy (LSG) and Roux-en-Y gastric bypass (RYGB), provide an avenue for exploring potential key factors involved in maintaining homeostasis since these surgeries have shown promising results improving glycemic among patients with severe type 2 diabetes (T2D). For first time, a markedly altered population of serum proteins after LSG was discovered analyzed...
Abstract The physical properties of cellular membranes, including fluidity and function, are influenced by protein lipid interactions. In situ labeling chemistries, most notably proximity-labeling interactomics well suited to characterize these dynamic often fleeting Established methods require distinct chemistries for proteins lipids, which limits the scope such studies. Here we establish a singlet-oxygen-based photocatalytic proximity platform (POCA) that reports intracellular interactomes...
Brown adipose tissue (BAT) is specialized for uncoupled heat production through mitochondrion fueled majorly from fatty acids (FAs) of lipid droplets (LDs). How the interaction between two organelles contributes generation remains elusive. Here, we report that LD-anchored mitochondria (LDAM) were observed in BAT mice raised at three different temperatures, 30 °C, 23 and 6 °C. The biochemical analyses including Western blotting electron transport chain subunits showed LDAM functional....
Extracellular vesicles are commonly found in human body fluids and can reflect current physiological conditions of act as biomarkers disease. The quality isolated extracellular facilitates the early diagnosis various diseases accompanied by hyperlipidemia. Nonetheless, there no reports on which special methods suitable for isolating from plasma patients with Thus, this study compared three different research-based vesicle isolation approaches, namely ultracentrifugation (UC), polyethylene...
Lipoproteins are complex particles comprised of a neutral lipid core wrapped with phospholipid monolayer membrane and apolipoproteins on the membrane, which is closely associated metabolic diseases. To facilitate elucidation its formation dynamics, as well applications, we developed an in vitro system adiposomes, consisting hydrophobic encircled by monolayer-phospholipid were engineered into artificial lipoproteins (ALPs) recruiting one or more kinds apolipoproteins, for example,...
Intestinal cholesterol absorption is an important contributor to systemic homeostasis. Niemann-Pick C1 Like 1 (NPC1L1), the target of drug ezetimibe (EZ), assists in initial step dietary uptake. However, how moves downstream NPC1L1 unknown. Here we show that Aster-B and Aster-C are critical for non-vesicular movement enterocytes, bridging at plasma membrane (PM) ACAT2 endoplasmic reticulum (ER). Loss diminishes accessible PM enterocytes abolishes Aster recruitment intestinal brush border....
Complex genetic and dietary cues contribute to the development of obesity, but how these are integrated on a molecular level is incompletely understood. Here, we show that PPARγ supports hypertrophic expansion adipose tissue via transcriptional control LPCAT3, membrane-bound O-acyltransferase enriches diet-derived omega-6 (
Abstract The plasma membrane proteome is a rich resource of functional and therapeutically relevant protein targets. Distinguished by high hydrophobicity, heavy glycosylation, disulfide-rich sequences, low overall abundance, the cell surface remains undersampled in established proteomic pipelines, including our own cysteine chemoproteomics platforms. Here we paired glycoprotein capture with to establish two-stage enrichment method that enables chemoproteomic profiling Surf ace Cys teinome....
The authors would like to update the supplementary information in published original version. updated material is provided this correction.
Abstract Over the past decade, majority of mammalian genome considered to be noncoding has been revealed able produce proteins. Many RNA molecules, mis‐annotated as noncoding, actually are predicted code for Some those proteins have identified and verified play critical roles in multiple biological processes. The lipid droplet (LD) is a unique cellular organelle bound with phospholipid monolayer membrane, closely associated metabolism metabolic disorders. However, it still unclear how...
Summary Brown adipose tissue (BAT) are specialized for uncoupled heat production through mitochondria fueled majorly from fatty acids (FA) of lipid droplets (LDs). How the interaction between two organelles contributes generation remains elusive. Here we report that LD-anchored (LDAM) were observed in BAT mice raised at three different temperatures, 30°C, 23°C, and 6°C. The biochemical analyses including Western blotting electron transport chain subunits showed LDAM functional given...