A5067920379- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Pregnancy and Medication Impact
- Renal Diseases and Glomerulopathies
- Hepatitis C virus research
- Immunotherapy and Immune Responses
- Pregnancy and preeclampsia studies
- RNA modifications and cancer
- Liver Disease Diagnosis and Treatment
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Mitochondrial Function and Pathology
- Systemic Lupus Erythematosus Research
- Proteoglycans and glycosaminoglycans research
- Reproductive Biology and Fertility
- Composting and Vermicomposting Techniques
- MicroRNA in disease regulation
- Immune cells in cancer
- Virus-based gene therapy research
- Prenatal Screening and Diagnostics
- Chronic Kidney Disease and Diabetes
- Inflammatory Biomarkers in Disease Prognosis
- HIV/AIDS drug development and treatment
- Tracheal and airway disorders
- Reproductive Physiology in Livestock
Chengdu University
2025
Fujian Normal University
2025
Sanya University
2025
Hainan University
2025
Shaoxing People's Hospital
2025
University of North Carolina at Chapel Hill
2019-2024
Guangdong Provincial Hospital of Traditional Chinese Medicine
2024
Shenzhen Zhongshan Urological Hospital
2014-2024
Lanzhou University
2024
South China Agricultural University
2024
Pentamidine, an antiprotozoal agent, has been traditionally known to cause QT prolongation and arrhythmias; however, its ionic mechanism not illustrated. In a stable HEK-293 cell line, we observed concentration-dependent inhibition of the hERG current with IC50 252 microM. freshly isolated guinea-pig ventricular myocytes, pentamidine showed no effect on L-type calcium at concentrations up 300 microM, slight action potential duration this concentration. Since effective channel APD were much...
<b><i>Background:</i></b> Acute kidney injury (AKI) is a frequent and serious complication of sepsis. A growing body evidence now suggests that inflammatory reactions tubular dysfunction induced by oxidative stress<b> </b>involved in the mechanisms disease. This study aimed to determine role anti-inflammatory anti-oxidant activities mangiferin (MA) sepsis-induced AKI. <b><i>Methods:</i></b> We investigated effects MA on apoptosis...
Background and Objective S100A8 S100A9, two members of the S100 protein family, have been reported in association with tumor cell differentiation progression. Previous study has showed that their expression stromal cells colorectal carcinoma (CRC) is associated size. Here, we investigated clinical significances S100A9 CRC underlying molecular mechanisms. Methods Expression matching distal normal tissues were measured by reverse transcriptase polymerase chain reaction (RT-PCR),...
Friedreich's ataxia is a rare disorder resulting from deficiency of frataxin, mitochondrial protein implicated in the synthesis iron-sulfur clusters. Preclinical studies mice have shown that gene therapy promising approach to treat individuals with ataxia. However, recent report provided evidence AAVrh10-mediated overexpression frataxin could lead cardiotoxicity associated dysfunction. While evaluating an AAV9-based using chicken β-actin promoter, we showed toxic be reached mouse liver and...
Polycystic ovary syndrome (PCOS), characterised by polycystic ovarian morphology, follicular maturation arrest, and hormonal disorders, affects numerous women in the reproductive age worldwide. A recent study has found that mitochondria are likely to play an essential role oocyte quality. However, it is still unclear whether development failure associated with patients PCOS. We analysed single-cell RNA sequencing data from previous study, including 14 oocytes 7 healthy fertile 20 9 PCOS at...
The Mediator complex (MED) functions as a co-activator in plants, transmitting transcriptional signals to regulate gene expression, including responses environmental stresses. While the MED family has been identified several species, it not yet reported cassava. In this study, we 32 members of MeMED cassava (Manihot esculenta Crantz) distributed across 13 chromosomes. These genes were categorized into distinct subunits based on their similarity Arabidopsis modules. Promoter analysis revealed...
To observe the efficacy of combination therapy with Penpulimab and Anlotinib in treatment retroperitoneal well-differentiated liposarcoma. Retrospective analysis clinical data a patient soft tissue sarcoma admitted to Shaoxing People’s Hospital, review relevant literature. The is young male who experienced recurrence liposarcoma after two surgeries. After first-line combined Penpulimab, achieved almost complete remission progression free survival period about 16 months. using resulted good prognosis.
Problem Human chorionic gonadotropin (hCG) and regulatory T cells (Tregs) have been suggested to play important roles during the initial stage of pregnancy. However, clinical relevance mechanism effects hCG on Treg functions in women with recurrent implantation failure (RIF) remain be elucidated. Method study Thirty-four RIF twenty-three control were included study. Endometrial peripheral Tregs analyzed by immunohistochemistry flow cytometry, respectively. generated from naïve CD4+...
Sustained, high level transgene expression in mammalian cells is desired many cases for studying gene functions. Traditionally, stable has been accomplished by using retroviral or lentiviral vectors. However, such viral vector-mediated often at low levels and can be reduced over time due to copy numbers and/or chromatin remodeling repression. The piggyBac transposon emerged as a promising non-viral vector system efficient transfer into cells. Despite its inherent advantages systems, not...
// Meng Wu 1, 2 , Xian Chen Jianfang Lou Shuping Zhang 3 Xiaojie Lei Huang Ruihong Sun Peijun Fang Wang Shiyang Pan 1 Department of Laboratory Medicine, The First Affiliated Hospital Nanjing Medical University, 210029, Nanjing, China National Key Clinical Children Hospital, Correspondence to: Wang, email: wangfang@njmu.edu.cn Pan, sypan@njmu.edu.cn Keywords: TGF-β1, p38 MAPK, CD8 + Treg, ovarian cancer Received: November 11, 2015 Accepted: May 29, 2016 Published: June 14, ABSTRACT regulatory...
Problem We aimed to investigate the modulatory effects of vitamin D on peripheral blood cellular immune response in patients with recurrent miscarriage ( RM ). Method study The effect number cells, T helper 1 (Th1) cytokines, and NK cytotoxicity was measured 99 women . Results percentage CD 19 + B cells at an effector‐to‐target cell (E:T) ratio 50:1, 25:1, 12.5:1 were significantly higher insufficiency group VDI ) than normal VDN P <.05 each). proportion TNF ‐α‐expressing Th deficiency...
Introduction Ovulation dysfunction is now a widespread cause of infertility around the world. Although impact immune cells in human reproduction has been widely investigated, systematic understanding changes atlas under female ovulation remain less understood. Methods Here, we generated single cell transcriptomic profiles 80,689 PBMCs three representative statuses dysfunction, i.e., polycystic ovary syndrome (PCOS), primary ovarian insufficiency (POI) and menopause (MENO), identified totally...
Immune dysfunction in early pregnancy including overactivation of cytotoxic CD16
<b><i>Objective:</i></b> In 2010, only 1 donor blood sample was found to be anti-hepatitis C virus (HCV) negative and HCV RNA positive, as detected by nucleic acid testing. occult infection (OCI), is in peripheral mononuclear cells (PBMCs). We investigated the prevalence of OCI among donors. <b><i>Methods:</i></b> collected 513 samples from 334 eligible 179 deferred donors, including 55 anti-HCV-positive, 113 alanine aminotransferase...
SUMMARY: Background/Aims: Injury and activation of tubular proximal epithelial cells (TEC) play central roles in renal tubulointerstitial fibrosis (TIF), but its mechanisms remain obscure. Interleukin 18 (IL‐18) is overproduced during chronic kidney diseases (CKD), how IL‐18 affects the biological behaviour TEC not clear. The aim present study to reveal role TIF. Methods: expressions receptor were detected by immunohistochemical staining vivo reverse transcriptase polymerase chain reaction...
microRNA(miR)-148b has been found to be downregulated in various human malignancies, including hepatocellular carcinoma (HCC) as well gastric, pancreatic, colon and oral cancer. However, the function of miR‑148b HCC remained elusive. The present study examined effects on proliferation, migration invasion cells vitro. After transfection HepG2 SMMC‑7721 cell lines with miR‑148b, an MTT assay, a Transwell assay western blot analysis were performed. miR-148b was shown inhibit two lines. Using...
Problem Dendritic cells ( DC s) have been reported to play an important role in pregnancy. However, the of s recurrent pregnancy loss RPL ) has not investigated well. Method study Forty‐three women affected by and 16 fertile controls were recruited from June 2013 December 2014. The peripheral blood subsets, including myeloid mDC plasmacytoid pDC s), levels (%) CD 80 + , 86 200 analyzed using flow cytometry. Results total s, significantly higher (all P <.05); however, level group was lower...
To explore the effect of early eschar dermabrasion combined with antimicrobial soft silicone foam dressing (hereinafter referred to as dressing) in treating deep partial-thickness burn wounds children.