A5031527634- Reproductive Biology and Fertility
- Prenatal Screening and Diagnostics
- Assisted Reproductive Technology and Twin Pregnancy
- Pluripotent Stem Cells Research
- Renal and related cancers
- Reproductive Health and Technologies
- CRISPR and Genetic Engineering
- Reproductive System and Pregnancy
- Tissue Engineering and Regenerative Medicine
- Single-cell and spatial transcriptomics
- Genetic Syndromes and Imprinting
- Analog and Mixed-Signal Circuit Design
- Angiogenesis and VEGF in Cancer
- Pregnancy and preeclampsia studies
- Orthopedic Surgery and Rehabilitation
- Mesenchymal stem cell research
- International Human Rights and Reproductive Law
- Cancer Immunotherapy and Biomarkers
- Muscle Physiology and Disorders
- Microfluidic and Bio-sensing Technologies
- Virus-based gene therapy research
- Demographic Trends and Gender Preferences
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Neurogenetic and Muscular Disorders Research
- Genetics and Neurodevelopmental Disorders
Nanjing Medical University
2015-2022
Tongji University
2013-2021
Jiangsu Province Hospital
2015-2020
Tongji Hospital
2016-2020
Capital Medical University
2020
Beijing Children’s Hospital
2020
Maternal and Child Health Care Hospital of Bao'an
2019-2020
University of California, Los Angeles
2008
Abstract Bioelectrical impulses intrinsically generated within the sinoatrial node (SAN) trigger contraction of heart in mammals. Though discovered over a century ago, molecular and cellular features SAN that underpin its critical function are uncharted territory. Here, we identify four distinct transcriptional clusters by single-cell RNA sequencing mouse SAN. Functional analysis differentially expressed genes identifies core cell cluster enriched electrogenic genes. The similar also...
Multipotent trophoblasts undergo dynamic morphological movement and cellular differentiation after conceptus implantation to generate placenta. However, the mechanism controlling trophoblast development during peri-implantation in human remains elusive. In this study, we modeled from blastocyst early postimplantation stages by using an vitro coculture system profiled transcriptome of 476 individual cells these conceptuses. We revealed genetic networks regulating development. While...
Polycystic ovary syndrome (PCOS), characterised by polycystic ovarian morphology, follicular maturation arrest, and hormonal disorders, affects numerous women in the reproductive age worldwide. A recent study has found that mitochondria are likely to play an essential role oocyte quality. However, it is still unclear whether development failure associated with patients PCOS. We analysed single-cell RNA sequencing data from previous study, including 14 oocytes 7 healthy fertile 20 9 PCOS at...
Fragile X syndrome (FXS) patients carry the expansion of over 200 CGG repeats at promoter fragile mental retardation 1 (FMR1), leading to decreased or absent expression its encoded protein (FMRP). However, global transcriptional alteration by FMRP deficiency has not been well characterized single nucleotide resolution, i.e., RNA-seq. Here, we performed in-vitro neuronal differentiation human induced pluripotent stem (iPS) cells that were derived from fibroblasts a FXS patient (FXS-iPSC). We...
Mesenchymal stem cells (MSCs) are increasingly recognized for their regenerative potential. However, clinical application is hindered by inherent variability, which influenced various factors, such as the tissue source, culture conditions, and passage number. MSCs were sourced from clinically relevant tissues, including adipose tissue-derived (ADMSCs, n = 2), chorionic villi-derived (CMMSCs, amniotic membrane-derived (AMMSCs, 3), umbilical cord-derived (UCMSCs, 3). Passages included cord at...
Genetic programs underlying preimplantation development and early lineage segregation are highly conserved across mammals. It has been suggested that nonhuman primates would be better model organisms for human embryogenesis, but a limited number of studies have investigated the monkey development. In this study, we collect single cells from cynomolgus embryos transcriptome profiling compare with single-cell RNA-seq data derived mouse embryos.By weighted gene-coexpression network analysis,...
BACKGROUND:Recent research indicates that CD133 are expressed in several kinds of stem cells, among which, its high expression laryngeal carcinoma has caused wide concern. To further explore efficaciously targeting drugs to cells (CSCs), we transplanted a solid tumor from CSCs into abdominal subcutaneous tissue nude mice, and then compared the biological characteristics tumors with or without cisplatin intervention. MATERIAL AND METHODS:In this study, was detected Hep-2 cell line by flow...
Abstract Multipotent trophoblasts undergo dynamic morphological movement and cellular differentiation after embryonic implantation to generate placenta. However, the mechanism controlling trophoblast development during peri-implantation remains elusive. In this study, we modeled human embryo from blastocyst early post-implantation stages by using an in vitro coculture system, profiled transcriptome of individual cells these embryos. We revealed genetic networks regulating development. While...
Abstract In mammals, female cells are obliged to inactivate one of two X chromosomes achieve dosage parity with the single chromosome in male cells, and it is also thought that active increased 2-fold balance sets autosomes (X:A ratio = 1, or Ohno’s hypothesis). However, ontogeny X-chromosome inactivation augmentation remains unclear during human embryogenesis. Here, we perform single-cell RNA-seq analysis examine timing X:A balancing X-inactivation (XCI) pre- peri-implantation embryos up...
Mitochondrion is a critical indicator of oocyte quality. The abnormality in mitochondrial quantity and function always leads to low quality oocytes or embryos assisted reproduction. mitochondria transplantation has become new method for improving the oocyte/embryo increasing success rate reproduction treatment. In this review, we explain roles that play maturation embryo development during preimplantation, highlight future application therapy treatment. Key words: Mitochondrial...
Embryo implantation involves complex contact between embryo and endometrium cells, which determines the following development of embryo. Synchronization blastocyst activation endometrial receptivity is key process for implantation. This signaling pathways regulating synchronization together with many unknown questions. review summarizes present research status exploring pathways. To elucidate mechanisms involving not only helps illuminate infertility in women but also may improve rate even...
Objective To analyze the clinical features and genetic characteristics of Chinese children with glutamate dehydrogenase type congenital hyperinsulinism (GDH-HI). Methods Pedigrees 10 GDH-HI admitted to Beijing Children′s Hospital from February 2008 December 2018 were selected as subjects. Clinical features, detection pathogenic genes follow-up data retrospectively analyzed. Polymerase chain reaction DNA (PCR-DNA) direct sequencing method second generation technique used GLUD1...