A5100453996- Ion channel regulation and function
- Autophagy in Disease and Therapy
- Cardiac electrophysiology and arrhythmias
- Cardiac Fibrosis and Remodeling
- Signaling Pathways in Disease
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Ion Transport and Channel Regulation
- Advanced Glycation End Products research
- Cardiac Ischemia and Reperfusion
- Liver Disease Diagnosis and Treatment
- Cardiomyopathy and Myosin Studies
- Neuroscience and Neuropharmacology Research
- Cardiovascular Function and Risk Factors
- Peroxisome Proliferator-Activated Receptors
- Pancreatic function and diabetes
- Congenital heart defects research
- Metabolism, Diabetes, and Cancer
- Adipose Tissue and Metabolism
- Extracellular vesicles in disease
- Nicotinic Acetylcholine Receptors Study
- Inflammasome and immune disorders
- Histone Deacetylase Inhibitors Research
First Affiliated Hospital of Xi'an Jiaotong University
2016-2025
Binzhou University
2024-2025
Binzhou Medical University
2024-2025
Shanghai Tenth People's Hospital
2024
Tongji University
2019-2024
Shenzhen University
2019-2024
Chongqing Medical University
2024
Union Hospital
2022-2024
Huazhong University of Science and Technology
2022-2024
Pudong Medical Center
2024
We demonstrate a role for the NAD-dependent deacetylase Sirt1 in regulation of autophagy. In particular, transient increased expression is sufficient to stimulate basal rates addition, we show that Sirt1(-/-) mouse embryonic fibroblasts do not fully activate autophagy under starved conditions. Reconstitution with wild-type but deacetylase-inactive mutant restores these cells. further can form molecular complex several essential components machinery, including genes (Atg)5, Atg7, and Atg8....
HIMF (hypoxia-induced mitogenic factor) is a secreted proinflammatory cytokine with critical role in cardiac hypertrophy development. Loss of attenuates transverse aortic constriction-induced and fibrosis, but the underlying mechanisms are unknown. We show that IL (interleukin)-6 production increases following constriction wild-type mice; this effect inhibited gene knockout ( Himf −/− ) mice. IL-6 also cultured myocytes overexpressing neutralizing an anti-IL-6 antibody prohibits HIMF-induced...
AimsThe cardiac ryanodine receptor (RyR) Ca2+ release channel homotetramer harbours ∼21 potentially redox-sensitive cysteine residues on each subunit and may act as a sensor for reactive oxygen species (ROS), linking ROS homeostasis to the regulation of signalling. In myocytes, arrayed RyRs or units are packed in close proximity mitochondria, primary source intracellular production. The present study investigated whether how mitochondria-derived regulate spark activity intact myocytes.
Alzheimer's disease is the most common type of dementia which characterized by progressive loss memory and cognitive function due to degeneration synapses axons. Currently, there no cure for disease. Deposition extracellular amyloid-β plaques intracellular neurofibrillary tangles are two major hallmark pathologic changes in brain patient. Autophagy mechanism cells responsible removing protein aggregates. Anormalous immature autophagic vacuoles accumulated dystrophic neurites Alzheimer...
The physiological role of autophagic flux within the vascular endothelial layer remains poorly understood. Here, we show that in primary cells, oxidized and native LDL stimulates autophagosome formation. Moreover, by both confocal electron microscopy, excess or modified appears to be engulfed structures. Transient knockdown essential autophagy gene ATG7 resulted higher levels intracellular (125) I-LDL (OxLDL) accumulation, suggesting may represent an important mechanism regulate excess,...
Abstract Bioelectrical impulses intrinsically generated within the sinoatrial node (SAN) trigger contraction of heart in mammals. Though discovered over a century ago, molecular and cellular features SAN that underpin its critical function are uncharted territory. Here, we identify four distinct transcriptional clusters by single-cell RNA sequencing mouse SAN. Functional analysis differentially expressed genes identifies core cell cluster enriched electrogenic genes. The similar also...
The multifunctional calcium/calmodulin-dependent protein kinase II, CaMKII, has been shown to regulate chloride movement and cellular function in both excitable non-excitable cells. We show that the plasma membrane expression of a member ClC family Cl−channels, human CLC-3 (hCLC-3), 90-kDa protein, is regulated by CaMKII. cloned full-length hCLC-3 gene from colonic tumor cell line T84, previously express CaMKII-activated Cl− conductance (ICl,CaMKII), transfected this into mammalian...
Pathological cardiac fibrosis and hypertrophy, the common features of left ventricular remodeling, often progress to heart failure. Forkhead box transcription factor P1 (Foxp1) in endothelial cells (ECs) has been shown play an important role development. However, effect EC-Foxp1 on pathological remodeling not well clarified. This study aims determine underlying mechanisms.Foxp1 EC-specific loss-of-function gain-of-function mice were generated, angiotensin II infusion or a transverse aortic...
HIMF (hypoxia-induced mitogenic factor/found in inflammatory zone 1/resistin like α) is a secretory and cytokine-like protein serves as critical stimulator of hypoxia-induced pulmonary hypertension. With role for heart disease unknown, we explored the possible roles cardiac hypertrophy by overexpressing knocking down cardiomyocytes characterizing gene (himf) knockout mice. We found that mRNA levels were upregulated phenylephrine-stimulated cardiomyocyte our mouse model transverse aortic...
The mitochondrial calcium uniporter is widely accepted as the primary route of rapid entry into mitochondria, where increases in matrix contribute to bioenergetics but also permeability and cell death. Hence, regulation activity critical homeostasis. subunit EMRE known be an essential regulator channel-forming protein MCU culture, EMRE's impact on organismal physiology less understood. Here we characterize a mouse model deletion show that indeed required for function vivo. EMRE-/- mice are...
The goals of this study are to investigate the mechanism and site action whereby a human ether-a-go-go-related gene (HERG)-specific scorpion peptide toxin, ErgTx, suppresses HERG current. We apply cysteine-scanning mutagenesis S5-P P-S6 linkers examine resulting changes in ErgTx potency. Data compared with characteristics charybdotoxin (ChTx, or its analogs) binding Shaker channel. binds outer vestibule but may not physically occlude pore. In contrast ChTx. interaction, elevating [K](o)...
Abstract Appropriately manipulating macrophage M1/M2 phenotypic transition is a promising therapeutic strategy for tissue repair after myocardial infarction (MI). Here we showed that gene ablation of hypoxia-induced mitogenic factor (HIMF) in mice ( Himf −/− and HIMF flox/flox ;Lyz2-Cre) attenuated M1 macrophage-dominated inflammatory response promoted M2 accumulation infarcted hearts. This turn reduced infarct size improved cardiac function MI. Correspondingly, expression macrophages...
Background: Previous studies have demonstrated that both vitamin C (VC) and D3 (VD3) therapeutic potential against metabolic disorders, including obesity, diabetes, metabolic-associated fatty liver disease (MAFLD). However, it is unclear whether VC supplementation associated with improving the intestinal flora regulating metabolism of bile acids via gut-liver axis in MAFLD. There still no direct comparison or combination study these two vitamins on effects. Methods: In this study, we...
Steatosis is a major risk factor for complications after liver surgery. Since neutrophil cytotoxicity critical ischemia-reperfusion injury in normal livers, the aim of present study was to evaluate whether an exaggerated inflammatory response could cause increased steatotic livers. In C57Bl/6 mice, 60 min warm hepatic ischemia triggered gradual increase accumulation during reperfusion with peak levels 100-fold over baseline at 12 h reperfusion. Neutrophil extravasation and specific...
Abstract Prostate cancer (PCa), the second most mortal from developed countries, presents a high level of chemoresistance. There is emerging evidence underscores critical role autophagy in onset, progression, and chemoresistance PCa. In present study, we investigated possible novel regulator, activating molecule beclin1-regulated autophagy1 (Ambra1), ATG gene sensitivity or PCa cells to cisplatin. We explored regulation by Ambra1 manipulation on induction apoptosis human DU145 presence...
Vascular smooth muscle cell (VSMC) proliferation is involved in many types of arterial diseases, including neointima hyperplasia, which Ca