A5101882276- Reproductive System and Pregnancy
- Cancer-related molecular mechanisms research
- Microfluidic and Bio-sensing Technologies
- Pregnancy and preeclampsia studies
- Reproductive Biology and Fertility
- Ovarian function and disorders
- Ferroptosis and cancer prognosis
- Cancer Immunotherapy and Biomarkers
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Lung Cancer Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- 3D Printing in Biomedical Research
- Renal cell carcinoma treatment
- Pleural and Pulmonary Diseases
- Epigenetics and DNA Methylation
- Kruppel-like factors research
- Bladder and Urothelial Cancer Treatments
- Endometriosis Research and Treatment
- Microfluidic and Capillary Electrophoresis Applications
- Fungal Infections and Studies
- Pelvic floor disorders treatments
- Cancer Genomics and Diagnostics
- DNA Repair Mechanisms
Renmin Hospital of Wuhan University
2019-2024
Wuhan University
2019-2024
Fujian Medical University
2012-2024
Second Affiliated Hospital of Fujian Medical University
2009-2024
Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in women reproductive age. miR-93-5p has been reported to be elevated granulosa cells PCOS patients. However, mechanism by which drives cell (GC) progression remains unclear. Thus, this study focuses on roles and mechanisms GCs PCOS. Methods KGN have similar ovarian physiological characteristics are used function regulatory GCs. In study, were transfected with si-NC, si-miR93-5p, oe-NC oe-miR93-5p. A counting...
Multicellular aggregates in three-dimensional (3D) environments provide novel solid tumor models that can insight into <italic>in vivo</italic> drug resistance.
Polycystic ovary syndrome (PCOS), which is often accompanied by insulin resistance, closely related to increased apoptosis of ovarian granulosa cells. LNK an important regulator the signaling pathway. When binds receptor, PI3K/AKT/FOXO pathway activated, and FOXO translocates from nucleus cytoplasm, thereby inhibiting expression pro-apoptotic genes.Granulosa cells were collected PCOS patients investigate relationship between LNK, cell resistance. KGN underwent overexpression/silence...
Abstract Precise and flexible three-dimensional (3D) cell construct assembly using external forces or fields can produce micro-scale cellular architectures with intercellular connections, which is an important prerequisite to reproducing the structures functions of biological systems. Currently, it also a substantial challenge in bioengineering field. Here, we propose smart acoustic 3D strategy that utilizes printed module hydrogel sheets. Digitally controlled six wave beams offer high...
Germline alterations in the breast cancer susceptibility genes type 1 and 2, BRCA1 BRCA2, predispose individuals to hereditary cancers, including breast, ovarian, prostate, pancreatic, stomach cancers. Accumulating evidence suggests inherited genetic lung cancer. The present study aimed survey prevalence of pathogenic germline BRCA mutations (gBRCAm) explore potential association between gBRCAm disease onset Chinese advanced non-small cell (NSCLC) patients.A total 6,220 NSCLC patients were...
Background. Endometrial cancer greatly threatens the health of female. Emerging evidences have demonstrated that DNA methylation and immune infiltration are involved in occurrence development endometrial cancer. However, mechanism prognostic biomarkers still unclear. In this study, we assess via bioinformatic analysis. Methods. The latest RNA-Seq, data, clinical data related to were downloaded from UCSC Xena dataset. methylation-driven genes selected, then risk score was obtained using...
Abstract Background Clinically, hormone replacement therapy (HRT) is the main treatment for primary ovarian insufficiency (POI). However, HRT may increase risk of both breast cancer and cardiovascular disease. Exosomes derived from human umbilical cord mesenchymal stem cell (hUC-MSC) have been gradually applied to a variety diseases through inflammation inhibition, immune regulation, tissue repair functions. application study hUC-MSC exosomes in POI remain limited. Methods Here, we first...
Background: Leptomeningeal metastases (LM), associated with poor prognosis, are frequent complications of advanced non-small cell lung cancer (NSCLC) patients, especially in patients epidermal growth factor receptor (EGFR) mutations. Due to limited access leptomeningeal lesions, the mutational landscape LM has not been comprehensively investigated large cohorts and underlining biology remains elusive. Some studies have explored potential cerebrospinal fluid (CSF) reflecting molecular profile...
A high-throughput cell-assembly method, with the advantages of adjustability, ease operation, and good precision, is remarkable for artificial tissue engineering. Here, we present a scientific solution by introducing high rotational symmetrical coherent acoustic waves, in order to enable shape arrangement potential wells be flexibly modulated, therefore assemble on large area diverse biomimetic arrays microfluidic platform. Ring arrays, honeycomb, many other are achieved real-time modulation...
Abstract Insufficient trophoblast invasion is the key factor for occurrence of recurrent spontaneous abortions (RSA). Our previous studies identified Yin Yang 1 (YY1) as a transcription involved in regulation invasiveness at maternal–fetal interface. Long noncoding RNAs (lncRNAs) can regulate gene expression and autophagy many ways. The purpose this study was to explore relationship between YY1 lncRNAs mechanism by which affect biological behavior trophoblasts. Bioinformatic analysis...
An acoustic streaming based rapid single-cell drug screening microfluidic device is developed. It achieved by non-invasively changing cell membrane permeability to accelerate absorption in concentration gradient field.
Abstract Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disease in women, with clinical manifestations of anovulation hyperandrogenaemia. The treatment PCOS mainly focuses on improving symptoms, such as insulin sensitivity or menstrual disorder, through drug treatment. However, due to the pathogenesis diversity PCOS, there still lack effective clinics. Metabolic disorder key factor occurrence PCOS. Brown adipose tissue (BAT) special human body that can participate...
Background: Cervical carcinoma is one of the most common malignant gynecological tumors and associated with high rates morbidity mortality. Early diagnosis early treatment can reduce mortality rate cervical cancer. However, there still no specific biomarkers for detection cancer prognosis. Therefore, it greatly urgent in searching correlated prognosis Results: The mRNA microRNA expression profile datasets (GSE7803, GSE9750, GSE63514, GSE30656) were downloaded from Gene Expression Omnibus...
Immune dysfunction in early pregnancy including overactivation of cytotoxic CD16
Cytotoxic T-lymphocyte associated protein 4 (CTLA4) inhibitors have been shown to significantly prolong the overall survival (OS) in a wide range of cancers. However, its application clear cell renal carcinoma (ccRCC) is limited due therapy response, and prognostic value CTLA4 ccRCC has not investigated detail. By using immunohistochemistry, Kaplan-Meier (K-M) analysis, uni- multi-variate Cox we comprehensively systematically studied ccRCC. Then, applied Gene Ontology (GO), Kyoto...
Hepatocellular carcinoma (HCC) ranks fifth among common cancers and is the second most cause of cancer-related mortality worldwide. This study aimed at identifying an immune-related long noncoding RNA (lncRNA) signature as a potential biomarker with prognostic value to improve early diagnosis provide therapeutic targets for HCC patients. The subjects this were samples complete transcriptome data clinical information downloaded from Cancer Genome Atlas (TCGA) database. We then extracted mRNA...
Hepatocellular carcinoma (HCC) is one of the main causes cancer-associated deaths globally, accounts for 90% primary liver cancers. However, further studies are needed to confirm metabolism-related gene signature related prognosis patients with HCC.Using "limma" R package and univariate Cox analysis, combined LASSO regression a was established. The relationship between overall survival (OS) HCC analyzed. RT-qPCR used evaluate expression genes in clinical samples. GSEA ssGSEA algorithms were...
Abstract In this study, we attempted to evaluate the prognostic value of infiltrating immune/stromal cells in clear cell renal carcinoma (ccRCC), by using immune scores and stromal based on “Estimation STromal Immune MAlignant Tumours Expression data” algorithm represent levels cells. We found that were associated with poor prognosis ccRCC. To assess role ccRCC cells, first, performed differentially expressed genes analysis functional for validation. The results showed underlying mechanism...
Polycystic ovary syndrome (PCOS) is the most common hormonal disorder among reproductive-aged women worldwide, however, mechanisms and progression of PCOS still unclear due to its heterogeneous nature. Using human granulosa-like tumor cell line (KGN) mice model, we explored function lncRNA UCA1 in pathological PCOS.CCK8 assay Flow cytometry were used do cycle, apoptosis proliferation analysis, results showed that knockdown KGN cells inhibited by blocking cycle promoted apoptosis. In vivo...
Intracellular cargo delivery is crucial for drug evaluation, nanomedicine development, and gene therapy, in which high efficiency while maintaining cell viability needed downstream analysis. Here, an acoustic-mediated precise delivering mechanism proposed by directly modulating micro-oscillation mode membrane permeability. Through phase shifting keying-based spatiotemporal acoustic tweezers, controllable oscillating arrays can be achieved shaking potentials. At the same time, continually...