A5101889796- Ginseng Biological Effects and Applications
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- MicroRNA in disease regulation
- Liver Disease Diagnosis and Treatment
- Lipid metabolism and disorders
- Adipokines, Inflammation, and Metabolic Diseases
- RNA Research and Splicing
- Chronic Kidney Disease and Diabetes
- Adipose Tissue and Metabolism
- Selenium in Biological Systems
- Renal Diseases and Glomerulopathies
- Phytoestrogen effects and research
- Pharmacological Effects of Natural Compounds
- Cancer-related Molecular Pathways
- Autophagy in Disease and Therapy
- Alcohol Consumption and Health Effects
- CRISPR and Genetic Engineering
- Pregnancy and preeclampsia studies
- Molecular Biology Techniques and Applications
- Pluripotent Stem Cells Research
- Hedgehog Signaling Pathway Studies
- Mesenchymal stem cell research
- Inflammasome and immune disorders
China Three Gorges University
2017-2025
Shenyang 242 Hospital
2024
University of Electronic Science and Technology of China
2024
Jiangxi Agricultural University
2024
College of Medical Sciences
2024
University of Chicago Medical Center
2017-2023
Molecular Oncology (United States)
2017-2023
Hormel (United States)
2013
University of Minnesota
2013
Hubei University
2009
The over-activation of the nucleotide-binding domain like receptor protein 3 (NLRP3) inflammasome plays an important role in pathogenesis ulcerative colitis (UC). Chlorogenic acid (CGA) exposure is identified as effective strategy for repressing inflammatory responses.In this study, NLRP3 model with LPS/ATP-induced RAW264.7 cells vitro and dextran-sulfate-sodium (DSS)-induced mice are used to evaluate effect CGA on inflammasome-related signaling. results suggest that suppressed expression...
Mesenchymal stem cells (MSCs) are multipotent non-hematopoietic progenitor that can undergo self-renewal and differentiate into multi-lineages. Bone marrow stromal (BMSCs) represent one of the most commonly-used MSCs. In order to overcome technical challenge maintaining primary BMSCs in long-term culture, here we seek establish reversibly immortalized mouse (imBMSCs). By exploiting CRISPR/Cas9-based homology-directed-repair (HDR) mechanism, target SV40T Rosa26 locus efficiently immortalize...
Effective bone tissue engineering is important to overcome the unmet clinical challenges as more than 1.6 million grafts are done annually in United States. Successful needs minimally three critical constituents: osteoprogenitor cells, osteogenic factors, and osteoinductive/osteoconductive scaffolds. Osteogenic progenitors derived from multipotent mesenchymal stem cells (MSCs), which can be prepared numerous sources, including adipose tissue. We previously showed that BMP9 most BMP induces...
Increasing evidence has demonstrated that hypoxia was an aggressive feature in endometrial cancer (EC), which is significantly associated with the tumor grade, lymph node metastasis, and resistance to chemotherapy. However, relationship between immune microenvironment EC not very clear. Exosomes are small membrane vesicles secreted from a variety of cell types mediate cell-to-cell communication through transported biomolecules. Here, we investigated whether exosomes can play immunomodulatory...
Selenium is an indispensable nutrient for plants, and optimizing selenium levels can enhance plant growth metabolism, leading to improved yield quality. In comparison conventional inorganic or organic fertilizers, nano-selenium demonstrates superior safety enhanced biological activity, making it more suitable crop production. Although fertilizer extensively used in various crops, its application pak choi remains limited. As a vital source of selenium, previous research on (Brassica chinensis...
Abstract Human mesenchymal stem cells (MSCs) are a heterogeneous subset of nonhematopoietic multipotent stromal and can differentiate into mesodermal lineage, such as adipocytes, osteocytes, chondrocytes, well ectodermal endodermal lineages. umbilical cord (UC) is one the most promising sources MSCs. However, molecular cellular characteristics UC‐derived MSCs (UC‐MSCs) require extensive investigations, which hampered by limited lifespan diminished potency over passages. Here, we used...
// Chengfu Yuan 1 , Chaoqi Liu Ting Wang Yumin He Zhiyong Zhou Yaoyan Dun Haixia Zhao Dongming Ren Junjie 2 Changcheng Zhang Ding College of Medical Science, China Three Gorges University, Yichang, HuBei 443002, Renhe Hospital Correspondence to: Yuan, email: yuancf46@ctgu.edu.cn Zhang, greatwall1@ctgu.edu.cn yuancf46@163.com Wang, junjiewang@ctgu.edu.cn Keywords: chikusetsu saponin IVa, adipose tissue inflammation, NLRP3 inflammasome, NF-κB signaling, ASC pyroptosome Received: January 23,...
The decreased renal function is known to be associated with biological aging, of which the main pathological features are chronic inflammation and interstitial fibrosis. In previous studies, we reported that total saponins from Panax japonicus (SPJs) can availably protect acute myocardial ischemia. We proposed SPJs might have similar protective effects for aging-associated Thus, in present study, evaluated overall effect on fibrosis.Sprague-Dawley (SD) aging rats were given by gavage...
Mesenchymal stem cells (MSCs) are multipotent and capable of differentiating into multiple cell types including osteoblastic, chondrogenic adipogenic lineages. We previously identified BMP9 as one the most potent BMPs that induce osteoblastic differentiation MSCs although exact molecular mechanism through which regulates osteogenic remains to be fully understood. Here, we seek develop a recombinant adenovirus system optimally silence mouse then characterize important role in MSCs. Using two...
The decline of aging brain neurons is the main cause various neurodegenerative disease. This study aimed to examine impact Balanophora polyandra polysaccharides (BPP) against related neuronal deterioration. C57BL/6 mice were fed with regular feed for 27 months establish a natural mouse model. From 3 age, in drug-treated group respectively containing 0.05 or 0.18% BPP until age. effects treatment on pathological changes evaluated, as well autophagy-related and signaling pathway proteins. had...
Renal interstitial fibrosis (RIF) is a crucial pathological change leading to chronic kidney disease (CKD). Currently, no effective medicines have been available for treating it. In our research, we examined the effects of polysaccharides extracted from Balanophora polyandra Griff (BPPs) on and epithelial mesenchymal transition (EMT) in vivo vitro, aiming explore underlying mechanisms. By using mice with unilateral urethral obstruction (UUO) as experimental subjects, medicinal values BPPs...
Cyclin-dependent kinase 4 (CDK4) is known to be a 33 kD protein that drives G1 phase progression of the cell cycle by binding CCND phosphorylate RB proteins. Using different CDK4 antibodies in western blot, we detected 2 groups proteins around 40 and kD, respectively, human mouse cells; each group often appeared as duplet or triplet bands. Some shRNAs could decrease wild-type (wt) but increase some proteins, whereas other had opposite effects. Liquid chromatography–mass spectrometry/mass...
While the human genome is pervasively transcribed, <2% of transcribed into protein-coding mRNAs, leaving most transcripts as noncoding RNAs, such microRNAs and long-noncoding RNAs (lncRNAs), which are critical components epigenetic regulation. lncRNAs emerging regulators gene expression genomic stability. However, it remains largely unknown about how regulated. Here, we develop a highly sensitive dynamic reporter that allows us to identify and/or monitor negative modulators lncRNA transcript...
Oxidative stress and mitochondrial dysfunction play a vital role in the pathogenesis of brain aging. Saponins from Panax japonicus (SPJ) have attracted much attention for their potential to attenuate age-related oxidative as main ingredient rhizomes japonicus.This study aimed investigate neuroprotective effects SPJ on natural aging rats well underlying mechanisms regarding pathway.Sprague-Dawley were divided into control groups (3-, 9-, 15- 24-month old groups) SPJ-treated groups. For...