A5084494815- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- Genomics and Chromatin Dynamics
- Bladder and Urothelial Cancer Treatments
- Cancer-related gene regulation
- Cancer Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Fatty Acid Research and Health
- Cancer Cells and Metastasis
- Caveolin-1 and cellular processes
- Head and Neck Cancer Studies
- Renal and related cancers
- MicroRNA in disease regulation
- Digestive system and related health
- CRISPR and Genetic Engineering
- Liver Disease Diagnosis and Treatment
- Fibroblast Growth Factor Research
- Ubiquitin and proteasome pathways
- Cancer survivorship and care
- Ferroptosis and cancer prognosis
- Advanced Breast Cancer Therapies
- Cancer, Hypoxia, and Metabolism
Sapienza University of Rome
2021-2024
University College London
2015-2023
Genomics England
2023
Cancer Institute (WIA)
2023
Cancer Research UK
2019-2022
CRUK Lung Cancer Centre of Excellence
2016
Hospital del Mar Research Institute
2015
Hospital Universitario Puerta del Mar
1997-2015
Municipal Institute for Medical Research
2008-2011
Hospital Del Mar
2008-2011
Expression of Snail1 in epithelial cells triggers an epithelial–mesenchymal transition (EMT). Here, we demonstrate that the synthesis Zeb2, a transcriptional repressor E-cadherin, is up-regulated after Snail1-induced EMT. does not affect Zeb2 mRNA, but prevents processing large intron located its 5′-untranslated region (UTR). This contains internal ribosome entry site (IRES) necessary for expression Zeb2. Maintenance 5′-UTR dependent on natural antisense transcript (NAT) overlaps 5′ splice...
Snail1 and Zeb1 are E-cadherin-transcriptional repressors induced during epithelial mesenchymal transition (EMT). In this article we have analyzed the factors controlling expression EMT. NMuMG cells treated with TGF-β, RNA protein 1 h after addition of cytokine preceding up-regulation that requires 6-8 h. gene is caused by increased levels but also enhanced stability markedly dependent on because depletion prevents up-regulation. to Snail1, Twist transcriptional factor retards stimulation...
Polycomb repressive complex 2 (PRC2) modifies chromatin to maintain genes in a repressed state during development. PRC2 is primarily associated with CpG islands at and also possesses RNA binding activity. However, the RNAs that bind cells, subunits mediate these interactions, role of recruitment all remain unclear. By performing iCLIP for comparison other proteins, we show here binds nascent essentially active genes. Although interacting promiscuously, enriched specific locations within...
The transcription factor SNAIL1 is a master regulator of epithelial to mesenchymal transition. very unstable protein, and its levels are regulated by the E3 ubiquitin ligase beta-TrCP1 that interacts with upon phosphorylation GSK-3beta. Here we show polyubiquitylation degradation may occur in conditions precluding GSK-3beta, suggesting additional ligases participate control protein stability. In particular, demonstrate F-box FBXl14 promotes ubiquitylation proteasome independently vivo,...
Circular RNAs (circRNAs) are widely expressed in eukaryotes and regulated many biological processes. Although several studies indicate their activity as microRNA (miRNA) protein sponges, little is known about ability to directly control mRNA homeostasis. We show that the circZNF609 interacts with mRNAs increases stability and/or translation by favoring recruitment of RNA-binding ELAVL1. Particularly, interaction CKAP5 mRNA, which interestingly overlaps back-splicing junction, enhances...
Abstract N6-Methyladenosine (m 6 A) is well-known for controlling different processes of linear RNA metabolism. Conversely, its role in the biogenesis and function circular RNAs (circRNAs) still poorly understood. Here, we characterize circRNA expression pathological context rhabdomyosarcoma (RMS), observing a global increase when compared to wild-type myoblasts. For set circRNAs, such an due raised m A machinery, which also find control proliferation activity RMS cells. Furthermore,...
Most studies of cohesin function consider the Stromalin Antigen (STAG/SA) proteins as core complex members given their ubiquitous interaction with ring. Here, we provide functional data to support notion that SA subunit is not a mere passenger in this structure, but instead plays key role localization diverse biological processes and promotes loading at these sites. We show cells acutely depleted for RAD21, remain bound chromatin, cluster 3D interact CTCF, well wide range RNA binding...
Abstract Pituitary adenomas comprise a heterogeneous subset of pathologies causing serious comorbidities, which would benefit from identification novel, common molecular/cellular biomarkers and therapeutic targets. The ghrelin system has been linked to development certain endocrine-related cancers. Systematic analysis the presence functional implications some components system, including native ghrelin, receptors recently discovered splicing variant In1-ghrelin, in human normal pituitaries...
Changes in DNA methylation of tumor suppressor genes early carcinogenesis represent potential indicators cancer detection and disease evolution. We examined the diagnostic, stratification prognostic biomarker roles urine a novel panel bladder cancer.We evaluated 18 2 prospective, independent sets samples (training set 120 preparations validation 128) from patients with (170) controls (78) using specific multiplex ligation-dependent probe amplification. Diagnostic performance was ROC curves....
AIM: To evaluate the relation between type of dietary fatty acid and degree insulin resistance. DESIGN: A cross-sectional study. METHODS: Anthropometrical data were measured in 538 subjects, aged 18-65 Years, selected randomly from municipal census Pizarra (Spain). An oral glucose tolerance test (OGTT) was given to all subjects measurements made glycemia, insulinemia proportion acids plasma phospholipids. Insulin resistance (IR) estimated by homeostasis model assessment. Samples cooking oil...
DNA methylation of tumor suppressor genes (TSGs) represents a frequent and early epigenetic event with potential applications for cancer detection disease evolution. Our aim was to examine the stratification prognostic biomarker role novel panel TSGs in bladder cancer. The status 18 evaluated cells (n=14) paraffin-embedded primary tumors (n=61), using methylation-specific multiplex ligation-dependent probe amplification assay (MS-MLPA). Recurrence, progression, disease-specific survival were...
Polycomb repressive complex 2 (PRC2) methylates histone H3 lysine 27 (H3K27me3) to maintain gene repression and is essential for cell differentiation. In low-grade endometrial stromal sarcoma (LG-ESS), the PRC2 subunit SUZ12 often fused with NuA4/TIP60 JAZF1. We show that JAZF1-SUZ12 dysregulates composition, genome occupancy, modification, expression, Loss of N terminus in fusion protein abrogates interaction specific accessory factors, reduces occupancy at target genes, diminishes...
Transcriptome analyses have revealed the existence of a large variety non-coding RNAs (ncRNAs) that, although specifically expressed, are largely unknown in function. The best-studied role ncRNAs is regulation gene expression, mostly participating transcription. We report here an antisense ncRNA that represses transcription LEF1 by recruiting Polycomb repressive complex 2 (PRC2), which trimethylates H3K27. This transcript undergoes splicing losing its inhibitory also discuss possible general...
Circular RNAs (circRNAs), a class of covalently closed formed by back-splicing reaction, have been involved in the regulation diverse oncogenic processes. In this article we describe circVAMP3, novel circular RNA overexpressed RH4, representative cell line alveolar rhabdomyosarcoma. We demonstrated that circVAMP3 has differential m6A pattern opposed to its linear counterpart, suggesting two isoforms can be differently regulated such modification. Moreover, show how depletion rhabdomyosarcoma...
In this report we have analyzed the role of antisense transcription in control LEF1 factor expression. A natural transcript (NAT) is transcribed from a promoter present first intron gene and undergoes splicing mesenchymal cells. Although locus silent epithelial cells, neither NAT nor mRNA are expressed, cell lines with an intermediate epithelial-mesenchymal phenotype presenting low expression, synthesized remains unprocessed. Contrarily to spliced NAT, unspliced down-regulates main activity...
Circular RNAs (circRNAs) are expressed and regulated in many biological processes but little is known about their ability to directly control mRNA homeostasis. We show that circRNA zinc finger protein 609 (circZNF609) interacts with several mRNAs increasing the final levels, which case of cytoskeleton-associated 5 (CKAP5) leads a stabilized microtubule cytoskeleton an enhanced tumor cell proliferation.
Circular RNAs (circRNAs), covalently closed that originate from back-splicing events, participate in the control of several processes, including those occur development pathological conditions such as cancer. Hereby, we describe circAFF1, a circular RNA overexpressed alveolar rhabdomyosarcoma. Using RH4 and RH30 cell lines, classical line models for rhabdomyosarcoma, demonstrated circAFF1 is cytoplasmatic circRNA its depletion impacts homeostasis favouring migration through downregulation...
ABSTRACT Most studies of cohesin function consider the Stromalin Antigen (STAG/SA) proteins as core complex members given their ubiquitous interaction with ring. Here, we provide functional data to support notion that SA subunit is not a mere passenger in this structure, but instead plays key role localization diverse biological processes and promotes loading at these sites. We show cells acutely depleted for RAD21, remain bound chromatin, cluster 3D interact CTCF, well wide range RNA...