Microwell technology has revolutionized many aspects of in vitro cellular studies from 2D traditional cultures to 3D vivo‐like functional assays. However, existing lithography‐based approaches are often costly and time‐consuming. This study presents a rapid, low‐cost prototyping method CO 2 laser ablation conventional untreated culture dish create concave microwells used for generating multicellular aggregates, which can be readily available general laboratories. Polymethylmethacrylate...

Abstract Despite significant advancements over the years, there remains an urgent need for low cost diagnostic approaches that allow rapid, reliable and sensitive detection of malaria parasites in clinical samples. Our previous work has shown magnetic resonance relaxometry (MRR) is a potentially highly tool diagnosis. A key challenge making MRR based diagnostics suitable testing fact baseline fluctuation exists between individuals, it difficult to detect level parasitemia. To overcome this...

The electrochemical behavior of iron ion in haemoglobin provides insight to the chemical activity red blood cell which is important field hematology. Herein, detection human cells on glassy carbon electrode (GC) was demonstrated. Red or raw immobilized a surface with Nafion films employed sandwich layer biological sample firmly surface. Cyclic voltammetry (CV) analyses revealed well-defined reduction peak for at about −0.30 V (vs. Ag/AgCl) (GC-Nf-RBC-3Nf) and (GC-Nf-B-3Nf) film modified GCE...

Diabetes mellitus is one of the fastest-growing health burdens globally. Oxidative stress, which has been implicated in pathogenesis diabetes complication (e.g., cardiovascular event), remains poorly understood. We report a new approach to rapidly manipulate and evaluate redox states blood using point-of-care NMR system. Various hemoglobin were mapped out newly proposed (pseudo) two-dimensional map known as T1-T2 magnetic state diagram. exploit fact that oxidative stress changes subtle...

This paper reports the fabrication and characterization of an adhesive-based liquid-metal microcoil for magnetic resonance relaxometry (MRR). Conventionally, microcoils are fabricated by various techniques such as electroplating, microcontact printing focused ion beam milling. These require considerable efforts incur high cost. In this paper, we demonstrate a novel technique to fabricate three-dimensional multilayer together with microfluidic network lamination dry adhesive sheets. One...

A novel, compact-sized (19 cm × 16 cm) and portable (500 g) magnetic resonance relaxometry system is designed developed. We overcame several key engineering barriers so that technology can be potentially used for disease diagnosis-monitoring in point-of-care settings, directly on biological cells tissues. The whole consists of a coin-sized permanent magnet (0.76 T), miniaturized radio-frequency microcoil probe, compact lumped-circuit duplexer, single board 1-W power amplifier, which field...

Translation of the findings in basic science and clinical research into routine practice is hampered by large variations human phenotype. Developments genotyping phenotyping, such as proteomics lipidomics, are beginning to address these limitations. In this work, we developed a new methodology for rapid, label-free molecular phenotyping biological fluids (e.g., blood) exploiting recent advances fast highly efficient multidimensional inverse Laplace decomposition technique. We demonstrated...

Abstract Low‐field nuclear magnetic resonance (NMR) relaxometry is an attractive approach from point‐of‐care testing medical diagnosis to in situ oil‐gas exploration. One of the problems, however, inherently long relaxation time (liquid) samples, (and hence low signal‐to‐noise ratio) which causes unnecessarily repetition time. In this work, a new class methodology presented for rapid and accurate object classification using NMR with aid machine learning. It demonstrated that sensitivity...

Mechanical properties of cells can be correlated with various cell states and are now considered as an important class biophysical markers. Effectiveness existing high-throughput microfluidic techniques for investigating mechanical is adversely affected by cell-size variation in a given population. In this work, we introduce new system real-time feedback control to evaluate single-cell deformability while minimizing dependence the measurement. Using breast cancer (MCF-7), demonstrate...

Abstract Malaria is major public health concerns which continues to claim the lives of more than 435,000 people each year. The challenges with anti-malarial drug resistance and detection low parasitaemia forms an immediate barrier achieve fast-approaching United Nations Sustainable Development Goals ending malaria epidemics by 2030. In this Opinion article, focusing on recent published technologies, in particularly nuclear magnetic resonance (NMR)-based diagnostic authors offer their...

Monitoring the direct electron transfer reactions of haemoglobin is important for understanding chemical changes within red blood cell haematological studies. However, facilitation between and bare solid electrodes challenging. Herein, influence carbon nanomaterials; graphite oxide (GO), chemically reduced graphene (CRGO), (GO′), electrochemically (ERGO) edge plane pyrolytic (EPPG); on in solution was investigated. We showed that GO, CRGO, GO′ ERGO did not exhibit improvement to...

Abstract Malaria continues to be among the most lethal infectious diseases. Immediate barriers include detection of low-parasitemia levels in asymptomatic individuals, which act as a reservoir for future infections, and emergence multidrug-resistant strains malaria-endemic, under-resourced regions. The development technologies field-deployable devices early targeted drugs/vaccines is an ongoing challenge. In this respect, identification hemozoin during Plasmodium growth cycle presents unique...

Abstract Olive oil is one of the oldest and essential edible oils in market. The classification olive (e.g. extra virgin, refined) often influenced by factors ranging from its complex inherent physiochemical properties fatty acid profiles) to undisclosed manufacturing processes. Therefore, have been target adulteration due profitable margin. In this work, we demonstrate that multi-parametric time-domain NMR relaxometry can be used rapidly (in minutes) identify classify label-free...

We aim to develop smoothed continuous 2.5th and 97.5th percentile values for labile glycated haemoglobin A1c (LHbA1c:HbA1c) ratio against HbA1c, apply them on our patient population identification of potentially spurious HbA1c measurements.The LHbA1c were measured using Bio-rad Variant II high-performance liquid chromatography system. recorded the 1555 patients who had normal chromatograms. Using these results, reference limits LHbA1c:HbA1c described by LHbA1c:HbA1c=-0.0072×HbA1c +0.2925...