A5032839194- Clinical Laboratory Practices and Quality Control
- Meta-analysis and systematic reviews
- Statistical Methods in Clinical Trials
- Hemodynamic Monitoring and Therapy
- Influenza Virus Research Studies
- Respiratory viral infections research
- Thyroid Disorders and Treatments
- Diabetes Management and Research
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Hemoglobinopathies and Related Disorders
- Neonatal Health and Biochemistry
- Metabolomics and Mass Spectrometry Studies
- Healthcare cost, quality, practices
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Hormonal and reproductive studies
- Reliability and Agreement in Measurement
- COVID-19 epidemiological studies
- Adipose Tissue and Metabolism
- Blood groups and transfusion
- Clinical Reasoning and Diagnostic Skills
- Scientific Measurement and Uncertainty Evaluation
- Renal function and acid-base balance
- Advanced Statistical Process Monitoring
- Health Systems, Economic Evaluations, Quality of Life
- SARS-CoV-2 detection and testing
National University Hospital
2016-2025
National University of Singapore
1997-2024
National University Health System
2012-2024
Sengkang General Hospital
2018-2019
Ministry of Health
2016
Bond University
2015
Singapore General Hospital
2013
University Health System
2012
KK Women's and Children's Hospital
2010-2011
RCSI & UCD Malaysia Campus
2008
Human blood is a self-regenerating lipid-rich biological fluid that routinely collected in hospital settings. The inventory of lipid molecules found plasma (plasma lipidome) offers insights into individual metabolism and physiology health disease. Disturbances the lipidome also occur conditions are not directly linked to metabolism; therefore, lipidomics based on MS an emerging tool array clinical diagnostics disease management. However, challenges exist translation such lipidomic data...
Abstract Despite intense interests in developing blood measurements of Alzheimer’s disease (AD), the progress has been confounded by limited sensitivity and poor correlation to brain pathology. Here, we present a dedicated analytical platform for measuring different populations circulating amyloid β (Aβ) proteins – exosome-bound vs. unbound directly from blood. The technology, termed mplified p lasmonic ex osome (APEX), leverages situ enzymatic conversion localized optical deposits...
Abstract Rapid, visual detection of pathogen nucleic acids has broad applications in infection management. Here we present a modular platform, termed enzyme-assisted nanocomplexes for identification (enVision). The system consists an integrated circuit enzyme–DNA nanostructures, which function as independent recognition and signaling elements, direct versatile from infected cells. built-in enzymatic cascades produce rapid color readout the naked eye; assay is thus fast (<2 h), sensitive...
Current technologies to subtype glioblastoma (GBM), the most lethal brain tumor, require highly invasive biopsies. Here, we develop a dedicated analytical platform achieve direct and multiplexed profiling of circulating RNAs in extracellular vesicles for blood-based GBM characterization. The technology, termed 'enzyme ZIF-8 complexes regenerative catalytic digital detection RNA' (EZ-READ), leverages an RNA-responsive transducer regeneratively convert catalytically enhance signals from rare...
Seven diverse primary isolates of human immunodeficiency virus type 1 (HIV-1) were examined and found to be refractory neutralization by antisera recombinant gp120 (rgp120) protein from HIV-1 MN. This stands in marked contrast the sensitivity exhibited certain laboratory-adapted viruses. To understand difference between viruses, we adapted ACH 168.10 growth FDA/H9 cell line. was chosen because V3 region closely matches that After 4 weeks, infection became evident. The (168A) replicated cells...
Abstract Serological testing for the detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is emerging as an important component clinical management patients with disease 2019 (COVID-19) well epidemiological assessment SARS-CoV-2 exposure worldwide. In addition to molecular infection, laboratories have also needed increase capacity include serological evaluation suspected or known COVID-19. While regulatory approved immunoassays are now widely available...
Abstract The diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection globally has relied extensively on molecular testing, contributing vitally to case identification, isolation, contact tracing, and rationalization control measures during the disease 2019 (COVID-19) pandemic. Clinical laboratories have thus needed verify newly developed tests increase testing capacity at an unprecedented rate. As COVID-19 pandemic continues pose a global health threat, continue...
An emerging technology (ET) for laboratory medicine can be defined as an analytical method (including biomarkers) or device (software, applications, and algorithms) that by its stage of development, translation into broad routine clinical practice, geographical adoption implementation has the potential to add value diagnostics. Considering medicine-specific definition, this document examines eight key tools, encompassing clinical, analytical, operational, financial aspects, used throughout...
Abstract Relatively few international comparisons of the incidence influenza related to climate parameters have been performed, particularly in Eastern hemisphere. In this study, and data such as temperature, relative humidity, rainfall, from cities at different latitudes with contrasting climates: Singapore, Hong Kong (China), Ulaanbaatar (Mongolia), Vancouver (Canada), three Australian (Brisbane, Melbourne Sydney) were examined determine whether there was any overall relationship between...
Human influenza viruses can be isolated efficiently from clinical samples using Madin-Darby canine kidney (MDCK) cells. However, this process is known to induce mutations in the virus as it adapts non-human cell-line. We performed a systematic study record pattern of MDCK-induced observed across whole A/H3N2 genome. Seventy-seven collected 2009-2011 were included study. Two full genomes obtained for each sample: one directly sample and matching isolate cultured MDCK Comparison full-genome...
Management of complex chronic diseases such as diabetes requires the assimilation and interpretation multiple laboratory test results. Traditional electronic health records tend to display results in a piecemeal segregated fashion. This makes assembly related care challenging. We developed diabetes-specific clinical decision support system (Diabetes Dashboard) interface for displaying glycemic, lipid renal function results, an integrated form with capabilities, based on local practice...
The prevalence of diabetes in Singapore is high. Screening to facilitate early detection and intervention has been shown be cost-effective. Current clinical practice guidelines recommend screening with fasting plasma glucose (FPG), followed by an oral tolerance test (OGTT) those FPG 6.1-6.9 mmol/L. Glycated haemoglobin A1c (HbA1c) robust stability at ambient temperature, can performed on non-fasted capillary blood samples, making it attractive potential alternative for screening. However,...