A5102019745- Advanced Fluorescence Microscopy Techniques
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Genomics and Chromatin Dynamics
- Click Chemistry and Applications
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Immune Response and Inflammation
- interferon and immune responses
- Chemical Synthesis and Analysis
- Advanced Electron Microscopy Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Neurogenetic and Muscular Disorders Research
- Lipid Membrane Structure and Behavior
- Anaerobic Digestion and Biogas Production
- Microtubule and mitosis dynamics
- Biotin and Related Studies
- Genetic Neurodegenerative Diseases
- Biofuel production and bioconversion
- Cellular transport and secretion
- Synthesis and biological activity
- Advanced Computational Techniques and Applications
- Glycosylation and Glycoproteins Research
University of California, San Francisco
2017-2022
Howard Hughes Medical Institute
2015-2021
Whitehead Institute for Biomedical Research
2020-2021
Moscow Institute of Thermal Technology
2021
IIT@MIT
2020
University of Illinois Urbana-Champaign
2009-2018
Center for Genomic Science
2014
Indian Institute of Technology Kharagpur
2009
National Centre for Biological Sciences
2009
Goethe University Frankfurt
2008
During intracellular membrane trafficking and remodeling, protein complexes known as the ESCRTs (endosomal sorting required for transport) interact with membranes are budding processes directed away from cytosol, including of intralumenal vesicles to form multivesicular bodies; some enveloped viruses; daughter cell scission in cytokinesis. We found that ESCRT-III proteins CHMP2A CHMP3 (charged body 2A 3) could assemble vitro into helical tubular structures expose their interaction sites on...
A-kinase anchoring proteins (AKAPs) tether the cAMP-dependent protein kinase (PKA) to intracellular sites where they preferentially phosphorylate target substrates. Most AKAPs exhibit nanomolar affinity for regulatory (RII) subunit of type II PKA holoenzyme, whereas dual-specificity also bind I (RI) with 10–100-fold lower affinity. A range cellular, biochemical, biophysical, and genetic approaches comprehensively establish that sphingosine interacting (SKIP) is a truly I-specific AKAP....
Abstract Fast opening and closing of voltage-gated sodium channels are crucial for proper propagation the action potential through excitable tissues. Unlike potassium channels, channel α-subunits believed to form functional monomers. Yet, an increasing body literature shows inconsistency with traditional idea a single α-subunit functioning as monomer. Here we demonstrate that not only physically interact each other but they actually assemble, function gate dimer. We identify region involved...
The mammalian target of rapamycin (mTOR) kinase is a master regulator cellular, developmental, and metabolic processes. Deregulation mTOR signaling implicated in numerous human diseases including cancer diabetes. functions as part either the two multisubunit complexes, mTORC1 mTORC2, but molecular details about assembly oligomerization mTORCs are currently lacking. We use single-molecule pulldown (SiMPull) assay that combines principles conventional assays with fluorescence microscopy to...
In eukaryotes, initiation of DNA replication requires the assembly a multiprotein prereplicative complex (pre-RC) at origins. We recently reported that WD repeat-containing protein, origin recognition (ORC)-associated (ORCA/LRWD1), plays crucial role in stabilizing ORC to chromatin. Here, we find ORCA is required for G(1)-to-S-phase transition human cells. addition binding ORC, associates with Cdt1 and its inhibitor, geminin. Single-molecule pulldown experiments demonstrate each molecule can...
Abstract Robust regulatory signals in the cell often depend on interactions between short linear motifs (SLiMs) and globular proteins. Many of these are poorly characterized because binding proteins cannot be produced amounts needed for traditional methods. To address this problem, we developed a single-molecule off-rate (SMOR) assay based microscopy fluorescent ligand to immobilized protein partners. We used it characterize substrate Anaphase-Promoting Complex/Cyclosome (APC/C), ubiquitin...
6-Oxopurine and its analogues form an important class of biological molecules that include nucleobases their precursors are substrates a wide range enzymes. Solution structures purines have been debated in the literature because many possible tautomers protonation states which they can exist solution. Substitutions on pyrimidine imidazole rings alter tautomerization equilibria, as consequence, solution compositions closely related be significantly different. We obtained resonance Raman...
Recognition of signaling phospholipids by proteins is a critical requirement for the targeting and initiation many cascades. Most biophysical methods measuring protein interactions with use purified proteins, which do not take into account effect post-translational modifications other cellular components on these interactions. To potentially circumvent problems, we have developed single-molecule fluorescence approach to analyzing lipid-protein in crude cell extracts. As proof principle this...
Progress towards a complete model of the methanogenic archaeum Methanosarcina acetivorans is reported. We characterized size distribution cells using differential interference contrast microscopy, finding them to be ellipsoidal with mean length and width 2.9 μ m 2.3 m, respectively, when grown on methanol 30% smaller acetate. used single molecule pull down (SiMPull) technique measure average copy number Mcr complex ribosomes. A kinetic for methanogenesis pathways based biochemical studies...
Key determinants in the emergence of complex cellular morphologies and functions are cues micro-environment. Primary among these is presence neighboring cells as networks form. Therefore, for high-resolution analysis, it crucial to develop micro-environments that permit exquisite control network formation. This especially true cell science, tissue engineering, clinical biology. We introduce a new approach assembling polydimethylsiloxane (PDMS)-based microfluidic environments enhances...