A5059529991- Microbial Community Ecology and Physiology
- Plant-Microbe Interactions and Immunity
- Mycorrhizal Fungi and Plant Interactions
- Methane Hydrates and Related Phenomena
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Soil Carbon and Nitrogen Dynamics
- Marine and coastal ecosystems
- Microbial bioremediation and biosurfactants
- Hydrocarbon exploration and reservoir analysis
- Conservation Techniques and Studies
- Drilling and Well Engineering
- Aquatic Ecosystems and Phytoplankton Dynamics
- Biochemical and biochemical processes
- Plant Disease Resistance and Genetics
- Wheat and Barley Genetics and Pathology
- Marine Biology and Ecology Research
- Hygrothermal properties of building materials
- Plant Parasitism and Resistance
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Polysaccharides and Plant Cell Walls
- Protist diversity and phylogeny
- Innovative Microfluidic and Catalytic Techniques Innovation
- Enzyme-mediated dye degradation
National Research Council Canada
2018-2023
McGill University
2015-2021
University of British Columbia
2011-2013
Bipar
2012
Portland State University
2008
Ifremer
2003-2004
Université du Québec à Rimouski
2004
Université du Québec à Montréal
2004
We present a programmable droplet-based microfluidic device that combines the reconfigurable flow-routing capabilities of integrated microvalve technology with sample compartmentalization and dispersion-free transport is inherent to droplets. The allows for execution user-defined multistep reaction protocols in 95 individually addressable nanoliter-volume storage chambers by consecutively merging sequences picoliter-volume droplets containing reagents or cells. This functionality enabled...
Oil in subsurface reservoirs is biodegraded by resident microbial communities. Water-mediated, anaerobic conversion of hydrocarbons to methane and CO2, catalyzed syntrophic bacteria methanogenic archaea, thought be one the dominant processes. We compared 160 community compositions ten hydrocarbon resource environments (HREs) sequenced twelve metagenomes characterize their metabolic potential. Although communities were common, cores from oil sands coal beds had unexpectedly high proportions...
Abstract Background A central challenge to understanding the ecological and biogeochemical roles of microorganisms in natural human engineered ecosystems is reconstruction metabolic interaction networks from environmental sequence information. The dominant paradigm assign functional annotations using BLAST. Functional are then projected onto symbolic representations metabolism form KEGG pathways or SEED subsystems. Results Here we present MetaPathways, an open source pipeline for pathway...
One method for rejuvenating land polluted with anthropogenic contaminants is through phytoremediation, the reclamation of cultivation specific crops. The capacity phytoremediation crops, such as Salix spp., to tolerate and even flourish in contaminated soils relies on a highly complex predominantly cryptic interacting community microbial life. Here, Illumina HiSeq 2500 sequencing de novo transcriptome assembly were used observe gene expression washed purpurea cv. 'Fish Creek' roots from...
Crop breeding has traditionally ignored the plant-associated microbial communities. Considering interactions between plant genotype and associated microbiota is of value since different genotypes same crop often harbor distinct communities which can influence phenotype. However, recent studies have reported contrasting results, led us to hypothesize that effect constrained by growth stages, sampling year compartment. To test this hypothesis, we sampled bulk soil, rhizosphere soil roots 10...
The objectives of this study were to uncover Salix purpurea-microbe xenobiotic degradation systems that could be harnessed in rhizoremediation, and identify microorganisms are likely involved these partnerships. To do so, we tested S. purpurea's ability stimulate the expression 10 marker microbial oxygenase genes a soil contaminated with hydrocarbons. In what appeared detoxification rhizosphere effect, transcripts encoding for alkane 1-monooxygenases, cytochrome P450 monooxygenases,...
Summary Thermocouple arrays were deployed on two deep‐sea hydrothermal vents at Guaymas Basin (27°0.5′N, 111°24.5′W) in order to measure situ temperatures which microorganisms colonize the associated mineral deposits. Intact sections of three structures that formed around collected after 4 and 72 day deployments (named BM4, BM72 TS72). Archaeal diversity with discreet subsamples across each deposit was determined by polymerase chain reaction amplification 16S rRNA genes. Spatial differences...
Many plants have natural partnerships with microbes that can boost their nitrogen (N) and/or phosphorus (P) acquisition. To assess whether wheat may undiscovered associations of these types, we tested if N/P-starved Triticum aestivum show microbiome profiles are simultaneously different from those N/P-amended and own bulk soils. The bacterial fungal communities root, rhizosphere, soil samples the Historical Dryland Plots (Lethbridge, Canada), which hold T. is grown both under N/P...
The oomycete pathogen, Aphanomyces euteiches , was implicated for the first time in pea and lentil root rot Saskatchewan Alberta 2012 2013. Subsequent surveys from 2014 to 2017 revealed that (ARR) widespread across Canadian prairies. absence of effective chemical, biological, cultural controls lack genetic resistance leave only one management option: avoidance. objectives this study were relate oospore levels autoclaved non-autoclaved soils ARR severity soil types vast prairie landscape...
Transcriptomic analysis has been used to compare the expression profiles of <italic>mlr</italic> gene cluster in newly isolated <italic>Sphingopyxis</italic> sp. MB-E.
ABSTRACT An extensive survey of the bacterial and archaeal phylogenetic diversity associated with a Paralvinella sulfincola tube adjacent substratum was conducted. Phylogenetic groups characterized by anaerobic and/or microaerophilic mesophiles dominated 16S rDNA libraries (ɛ‐group F, Sulfurospirillum ‐group, δ‐ Proteobacteria ). The two also contained thermophilic lifestyles D, Caldithrix ‐group), comprising species various phenotypes B, green non‐sulfur bacteria), group without cultured...
Poly(itaconic acid) (PIA) is a renewable, biobased, hydrophilic polymer of superabsorbent properties, with the potential to replace synthetic polymers in agricultural applications. Herein, we tested PIA for encapsulation two model plant growth-promoting bacteria (PGPB), Bacillus subtilis and Pseudomonas fluorescens, using spray drying. Linear was prepared, mixed PGPB, spray-dried form microbe-loaded microspheres. The latter were analyzed mass yield, moisture content, morphology, microbial...
Abstract Crop breeding has traditionally ignored the plant-associated microbial communities. Consideration of interactions between plant genotype and associated microbiota is value since different genotypes same crop often harbor distinct communities which can influence phenotype. However, recent studies have reported contrasting results, led us to hypothesize that effect constrained by time (growth stage, year) space (plant compartment). To test this hypothesis, we sampled bulk soil,...
Many plants have natural partnerships with microbes that can boost their nitrogen (N) and/or phosphorus (P) acquisition. To assess whether wheat may undiscovered associations of these types, we tested if N/P-starved Triticum aestivum show microbiome profiles are simultaneously different from those N/P-amended and own bulk soils. The bacterial fungal communities root, rhizosphere, soil samples the Historical Dryland Plots (Lethbridge, Canada), which hold T. is grown both under N/P...