A5055255364- Atherosclerosis and Cardiovascular Diseases
- Antioxidant Activity and Oxidative Stress
- Lipoproteins and Cardiovascular Health
- Lipid metabolism and biosynthesis
- Cholesterol and Lipid Metabolism
- Peroxisome Proliferator-Activated Receptors
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Oral microbiology and periodontitis research
- Cell Adhesion Molecules Research
- Retinoids in leukemia and cellular processes
- Heme Oxygenase-1 and Carbon Monoxide
- Protease and Inhibitor Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Coronary Interventions and Diagnostics
- Inflammatory mediators and NSAID effects
- Adipose Tissue and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Calpain Protease Function and Regulation
- Retinal Diseases and Treatments
- Cardiac Imaging and Diagnostics
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Liver Disease Diagnosis and Treatment
- Lipid Membrane Structure and Behavior
- Cardiovascular Disease and Adiposity
- Photosynthetic Processes and Mechanisms
Showa University
2015-2024
Showa Pharmaceutical University
2006-2024
Teikyo University
1998-2009
Tokushima University
2002-2008
Hoshi University
2008
Niigata University of Pharmacy and Medical and Life Sciences
2007
Osaka City General Hospital
2001-2006
Osaka City University
2001-2006
University of Amsterdam
2001-2006
Amsterdam UMC Location University of Amsterdam
2001-2006
Background —There is accumulating data that acute coronary syndromes relate to recent onset activation of inflammation affecting atherosclerotic plaques. Increased blood levels oxidized low density lipoprotein (ox-LDL) could play a role in these circumstances. Methods and Results —Ox-LDL were measured 135 patients with myocardial infarction (AMI; n=45), unstable angina pectoris (UAP; stable (SAP; n=45) 46 control subjects using sandwich ELISA method. In addition, 33 atherectomy specimens...
We recently cloned monoclonal IgM autoantibodies which bind to epitopes of oxidized low-density lipoprotein (OxLDL) from apoE-deficient mice (EO– autoantibodies). now demonstrate that those EO– were originally selected for binding copper-oxidized lipoproteins (CuOx-LDL), also bound both the protein and lipid moieties CuOx-LDL. The same showed specific products 1-palmitoyl-2-arachidonoyl-phosphatidylcholine (OxPAPC) phospholipid, 1-palmitoyl-2-(5-oxovaleroyl)-phosphatidyl-choline (POVPC),...
Background Although long-term lipid-lowering therapy improves endothelium-dependent vasodilatation in humans, it remains unknown whether the short-term removal of LDL per se ameliorates endothelial dysfunction. Methods and Results To examine effects a single session apheresis on function patients with hypercholesterolemia, we measured forearm blood flow (FBF) by strain-gauge plethysmography before after while infusing acetylcholine (ACh; 4 to 24 μg/min) sodium nitroprusside (SNP; 0.2 1.2...
Abstract —Recent studies have established oxidative modification of low density lipoprotein (LDL) as an important atherogenic factor. We examined the clinical relevance circulating oxidized LDL (OxLDL) levels in atherosclerotic disease by enzyme immunoassay with use specific antibodies against OxLDL (FOH1a/DLH3) and apolipoprotein B. Plasma were significantly higher patients coronary heart (n=65) than control subjects (n=181; 201.3±11.2 versus 112.4±3.3 U/dL, respectively; P <0.01). not...
Objective— Oxidation of LDL plays a significant pathogenic role in atherosclerosis. In this study, we attempted to clarify the correlation between morphology human atherosclerotic plaques and oxidized (OxLDL) levels plasma carotid plaques. Methods Results— OxLDL (ng/μg apolipoprotein B) from 44 patients undergoing endarterectomy 17 control 9 normal intima samples were determined by sandwich ELISA using specific antibodies against (DLH3) B. The immunohistochemically classified as macrophage...
Abstract —Heme oxygenase-1 (HO-1) is induced by a variety of conditions associated with oxidative stress. We demonstrated that mildly oxidized LDL markedly induces HO-1 in human aortic endothelial and smooth muscle cell cocultures its induction results the attenuation monocyte chemotaxis resulting from treatment vitro. To elucidate role development atherosclerotic lesions vivo, we modulated expression LDL-receptor knockout mice fed high-fat diets. During 6-week diet trials, intraperitoneal...
In this study we have used homogenates of human atheromatous plaque as immunogen to establish a murine monoclonal antibody which recognizes oxidized low density lipoprotein (LDL). This antibody, FOH1a/DLH3, reacted with LDL, but enzyme-linked immunosorbent assay showed that it had no reaction native, acetylated, or malonaldehyde-treated LDL. The cross-reacted high lipoprotein, suggesting specific sequences the apolipoprotein B are not essential for antigen recognition by antibody....
We have established a new method capable of measuring the very low concentrations oxidized density lipoprotein (OxLDL). In our previous study, we obtained novel murine monoclonal antibody against lipoproteins (Itabe, H. et al. 1994. J. Biol. Chem. 269: 15274-15279). The epitope this resides in products phosphatidylcholine that can form complexes with polypeptides, including apolipoprotein B. When was precoated onto microtiter wells prior to carrying out sandwich ELISA using an anti-human B...
Heme oxygenase-1 (HO-1), the rate-limiting enzyme of heme degradation, has recently been considered to have protective roles against various pathophysiological conditions. Since we demonstrated that HO-1 overexpression inhibits atherosclerotic formation in animal models, examined effect HO modulation on proinflammatory cytokine production, endothelial NO synthase (eNOS) expression, and endothelium-dependent vascular relaxation responses.After induction by arginate (HA), cell cultures were...
Background —Patients who undergo hemodialysis experience accelerated atherosclerosis and premature death. Recent evidence suggests that endothelial dysfunction proceeds to exacerbates atherosclerosis. It remains unknown whether per se causes dysfunction. Methods Results —We evaluated function estimated by flow-mediated vasodilation during reactive hyperemia using high-resolution ultrasound Doppler echocardiography before after a single session in patients on maintenance hemodialysis. Several...
Collectins are a family of C-type lectins that have collagen-like sequences and carbohydrate recognition domains (CRD). They involved in host defense through their ability to bind antigens microorganisms. The scavenger receptors type A MARCO classical internal domains. Here we describe new receptor is membrane-type collectin from placenta (collectin 1 (CL-P1)), which has typical domain CRD. cDNA an insert about 2.2 kilobases coding for protein containing 742 amino acid residues. deduced...
Lipid droplets (LDs) function as intracellular storage depots of neutral lipids. Recently, we identified long-chain acyl-coenzyme A synthetase 3 (ACSL3) a major LD-associated protein in the human hepatocyte cell line HuH7. In this study, investigated whether droplet-associated ACSL is involved lipid metabolism LDs. Addition oleic acid (OA) to culture medium was shown enhance accumulation LDs cells, which accompanied by an increase droplet ACSL3. When LD-enriched cells induced OA were further...
Accumulating evidence indicates that oxidized low-density lipoprotein (OxLDL) is a useful marker for cardiovascular disease. The uptake of OxLDL by scavenger receptors leads to the accumulation cholesterol within foam cells atherosclerotic lesions. has many stimulatory effects on vascular cells, and presence in circulating blood been established. According classical hypothesis, accumulates lesions over long duration, leading advanced However, recent studies time-course changes vivo raised...
Age-related macular degeneration (AMD) is the leading cause of blindness among elderly in developed countries. Although pathogenic factors, such as oxidative stress, inflammation and genetics are thought to contribute development AMD, little known about relationships priorities between these factors. Here, we show that chronic photo-oxidative stress an environmental factor involved AMD pathogenesis. We first demonstrated exposure light induced phospholipid oxidation mouse retina, which was...
Objective— NADH/NADPH oxidase is an important source of reactive oxygen species (ROS) in the vasculature. Recently, we demonstrated that p22 phox , essential component this oxidase, was expressed human coronary arteries and its expression enhanced with progression atherosclerosis. The present study undertaken to investigate functional importance pathogenesis artery disease. For aim, distribution oxidized low density lipoprotein (LDL), generation ROS directional atherectomy (DCA) specimens...
Nonalcoholic steatohepatitis/nonalcoholic fatty liver disease is considered to be a hepatic manifestation of various metabolic disorders. However, its precise pathogenic mechanism obscure. Oxidative stress and consequent lipid peroxidation seem play pivotal role in progression. In this study, we analyzed the localization oxidized phosphatidylcholine (oxPC), peroxide that serves as ligand for scavenger receptors, livers patients with steatotic disorder. Specimens non-alcoholic (15 autopsy...
Among the many mammalian secreted phospholipase A2 (sPLA2) enzymes, PLA2G3 (group III A2) is unique in that it possesses unusual N- and C-terminal domains its central sPLA2 domain homologous to bee venom PLA2 rather than other sPLA2s. To elucidate vivo actions of this atypical sPLA2, we generated transgenic (Tg) mice overexpressing human PLA2G3. Despite marked increases activity mature 18-kDa protein circulation tissues, Tg displayed no apparent abnormality up 9 months age. However,...
Oxidatively modified low density lipoprotein (OxLDL) is known to be involved in atherogenesis. We have previously developed a murine monoclonal antibody, FOH1a/DLH3, which recognized oxidatively lipoproteins as well foam cells human atherosclerotic lesions (Itabe, H., Takeshima, E., Iwasaki, Kimura, J., Yoshida, Y., Imanaka, T., and Takano, T. (1994) J. Biol. Chem. 269, 15274-15279). The antigen of this antibody was formed by peroxidation phosphatidylcholine (PC), the antigenic oxidized PC...