A5069277076- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Advanced MRI Techniques and Applications
- Advanced Neuroimaging Techniques and Applications
- Biblical Studies and Interpretation
- Receptor Mechanisms and Signaling
- Peptidase Inhibition and Analysis
- Diabetes Treatment and Management
- Functional Brain Connectivity Studies
- Congenital Diaphragmatic Hernia Studies
- Congenital Heart Disease Studies
- Crystallography and molecular interactions
- Pentecostalism and Christianity Studies
- Digital Mental Health Interventions
- Historical and Linguistic Studies
- MRI in cancer diagnosis
- Impact of Technology on Adolescents
- Telemedicine and Telehealth Implementation
- Pluripotent Stem Cells Research
- Computational Drug Discovery Methods
- Tracheal and airway disorders
- Pulmonary Hypertension Research and Treatments
- Inflammatory mediators and NSAID effects
- Animal Genetics and Reproduction
- Patient Satisfaction in Healthcare
University of California, Davis
2018-2025
Mayo Clinic in Florida
2022-2024
Mayo Clinic Health System
2024
Mayo Clinic
2022-2023
Hill Engineering (United States)
2023
Bryant University
2023
Smith Family
2023
University of Colorado Anschutz Medical Campus
2020-2022
WinnMed
2022
University of Colorado Denver
2021
In this study, we established bovine embryonic stem cell (bESC) lines from early (eBL) and full (BL) blastocysts to determine the efficiency of bESC derivation an earlier stage compare characteristics resulting lines. Using medium protocols for primed bESCs expanded blastocysts, derived eBLs BLs with same (4/12 each, 33%). Regardless original blastocyst stage, had a similar phenotype, including differentiation capacity, stable karyotype, pluripotency marker expression over feeder-free...
Recovery of function following traumatic brain injury (TBI) is partly through neuronal plasticity. However plasticity limited in the adult CNS compared with young animals. In order to test whether treatments that enhance might improve functional recovery after TBI, a new rat head model was developed, which computer-controlled impactor produced full thickness lesions forelimb region sensorimotor cortex. Behavioural deficits were seen several tasks, most recovered spontaneously by 21 days....
Abstract Background The homologous recombination (HR) pathway is largely inactive in early embryos prior to the first cell division, making it difficult achieve targeted gene knock-ins. homology-mediated end joining (HMEJ)-based strategy has been shown increase knock-in efficiency relative HR, non-homologous (NHEJ), and microhomology-mediated (MMEJ) strategies non-dividing cells. Results By introducing gRNA/Cas9 ribonucleoprotein complex a HMEJ-based donor template with 1 kb homology arms...
1.Time-lapse diffusion-weighted magnetic resonance imaging (DWI) was used to detect and characterize complex waves of cortical spreading depression (CSD) evoked with KCL placed upon the suprasylvian gyrus anaesthetized cats. 2. The time-lapse representations successfully demonstrated primary CSD that propagated elliptical wavefronts selectively over ipsilateral cerebral hemispheres a velocity 3.8 +/- 0.70 mm min(-1) (mean S.E.M. 5 experiments). 3. In contrast, succeeding secondary often...
In the fifteen years since explosion of Internet, using cyber technology for work and social functions has exponentially increased. Yet, questions around how to manage such changes remain elusive in family therapy literature. this investigation, we conducted a content analysis determine what extent marriage/couple (M/CFT) journals have responded integration Internet couple life. We found 79 13,274 articles across seventeen focused on some capacity. Implications clinical practice, training,...
This volume draws a map of British film culture in the 1970s and provides wide-ranging history period. It examines cross-cultural relationship between cinema other media, including popular music television. The analysis covers mainstream experimental cultures, identifying their production contexts economic, legislative censorship constraints on throughout decade. essays Part I contextualise study illustrate diversity moving image culture. In II, Sue Harper Justin Smith examine how gender...
The production of the lipoxygenase metabolite arachidonic acid, leukotriene B4 (LTB4) by normal rat glomeruli has been studied. Isolated from saline-perfused kidneys were incubated in Kreb's buffer for 15 min at 37 degrees C. concentrations LTB4 and other eicosanoids cell-free supernatants determined direct radioimmunoassays (RIA). Mean basal syntheses were: thromboxane B2 (TXB2) 0.77 +/- 0.13, prostaglandin (PG) E2 0.40 0.05, 6-keto-PGF1 alpha 0.38 0.06 ng/mg glomerular protein (mean SEM n...
OBJECTIVE: Certifications in psychiatric-mental health nursing promote safe practice by nurse practitioners (PMHNPs) and nurses (PMHNs) help protect the public from harm. This protection begins with development of an examination that meets rigorous national education, practice, accreditation standards reflects PMHNPs’ or PMHNs’ clinical practice. Achievement maintenance a certification is journey involves commitment to lifelong learning improvement field through involvement process. METHODS:...
Cortical spreading depression (CSD) in the gyrencephalic cat brain was detected with diffusion-weighted echoplanar (DWEP) magnetic resonance imaging (4–8/min for 1–2 hours) using a horizontal plane through suprasylvian (SG) and marginal gyri. A t-statistic mapping technique allowed quantitative characterization of passage events single-image pixels (0.15 mm2), thus providing resolution unavailable to previous studies which time-dependent changes instead were derived from averaging data over...
Cortical spreading depression (CSD) was induced by transient (10 min) applications of KCl in agar upon the cortical surface alpha-chloralose anaesthetised cats. Its features were compared with CSD resulting from sustained crystalline through a mapping apparent diffusion coefficient (ADC) using diffusion-weighted echo planar imaging (DWI) over poststimulus period 60-100 min. Individual events computationally detected aid Savitzky-Golay smoothing applied to critically sampled data derived...
Cortical spreading depression (CSD) and peri‐infarct depolarisation (PID) are related phenomena that have been associated with the human clinical syndromes of migraine (CSD), head injury stroke (PID). Nevertheless existence CSD in man remains controversial, despite detection this phenomenon brains most, if not all, other animal species investigated. This failure to unambiguously detect clinically may be at least partly due anatomically complex, gyrencephalic structure brain. study was...
Simple molecular models predict key aspects of the "microscopic" assembly behavior various peptide systems in fabrication multilayer films. Such films show substantial differences density for different systems. The data suggest that exponential film growth is possible absence polymer diffusion and "macroscopic" more a function peptide-peptide interactions than sequence alone.
Abstract Pulmonary Arterial Hypertension (PAH) is overrepresented in People Living with Human Immunodeficiency Virus (PLWH). HIV protein gp120 plays a key role the pathogenesis of HIV-PAH. Genetic changes determine viral interactions chemokine receptors; specifically, HIV-X4 viruses interact CXCR4 while HIV-R5 CCR5 co-receptors. Herein, we leveraged banked samples from patients enrolled NIH Lung studies and used bioinformatic analyses to investigate whether signature sequences HIV- that...
Cortical spreading depression (CSD) and peri-infarct depolarisation (PID) are related phenomena that have been associated with the human clinical syndromes of migraine (CSD), head injury stroke (PID). Nevertheless existence CSD in man remains controversial, despite detection this phenomenon brains most, if not all, other animal species investigated. This failure to unambiguously detect clinically may be at least partly due anatomically complex, gyrencephalic structure brain. study was...
Marek's disease (MD) is an infectious characterized by lymphomas and high mortality in susceptible chickens. The causative ubiquitous alpha-herpesvirus known as MD virus (MDV) integrates into host telomeres during early infection through latency, to be important phase for oncogenic transformation. Herein, we sought determine the influence of vaccination genetics on temporal dynamics MDV-host genome interactions. We studied integration profiles using 2 vaccines that vary protective efficacy...
People living with HIV and who receive antiretroviral therapy have a significantly improved lifespan, compared to the early days without therapy. Unfortunately, persisting viral replication in lungs sustains chronic inflammation, which may cause pulmonary vascular dysfunction ultimate life-threatening Pulmonary Hypertension (PH). The mechanisms involved progression of PH remain unclear. study HIV-PH is limited due lack tractable animal models that recapitulate infection pathobiological...
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