Runx2 is a critical transcription factor for osteoblast differentiation. Regulation of expression levels and transcriptional activity important bone morphogenetic protein (BMP)-induced Previous studies have shown that extracellular signal-regulated kinase (Erk) activation enhances the BMP-induced acetylation increases stability activity. Because BMP signaling induces Erk in osteoblasts, we sought to investigate whether regulates stability. by overexpression constitutively active MEK1...

Variation in content of organic acids and soluble sugars, physical characteristics was evaluated apricot ( P. armeniaca L. cv. Harcot), plumcot (plum-apricot hybrid, salicina ⅹ Harmony), plum Lindl. Formosa), peach persica Batsch Jinmi). The as well parameters fruit quality (weight, dimensions, firmness, total solids, acidity) Prunus fruits during development were determined. Organic acids, including oxalic acid, quinic malic shikimic citric sucrose, fructose, glucose, sugar alcohol...

Acute kidney injury frequently occurs after cardiac surgery, and is primarily attributed to renal ischemia-reperfusion (I/R) inflammation from surgery cardiopulmonary bypass. Vitamin C, an antioxidant that often depleted in critically ill patients, could potentially mitigate I/R-induced oxidative stress at high doses. We investigated the effectiveness of high-dose vitamin C preventing injury. The ideal time optimal dosage for administration were determined a two-phase experiment on...

Abstract Fracture of the alveolar bone resorption is a common complication in orthodontic treatment, which mainly caused by extreme mechanical loading. However, ferroptosis with tooth movement(OTM) relationship has not been thoroughly described. We here analysed whether involved OTM‐associated loss. Mouse osteoblasts (MC‐3T3) and knockdown glutathione peroxidase 4 (GPX4) MC‐3T3 were stimulated compressive force loading ferrostatin‐1 (Fer‐1, inhibitor), changes lipid peroxidation morphology,...

Lidocaine exerts potential anti-tumor effects on various cancer cell lines, and its intravesical instillation is considered safer than intravenous administration for bladder cancer. However, the mechanisms underlying have not been fully elucidated. Here, we aimed to elucidate molecular of lidocaine in cells a xenograft model substantiate efficacy administration. We investigated anti-proliferative autophagyinducing activities Nara Bladder Tumor No. 2 (NBT-II) rat carcinoma using viability,...

Abstract Estrogen deficiency causes osteoporosis via increased generation of reactive oxygen species (ROS), and thus, antioxidants may prove to be the effective therapeutic candidates. We examined effects antioxidant N ‐acetylcysteine (NAC) on osteoblastic differentiation in mouse calvarial cells. NAC (10–30 mM) enhanced alkaline phosphatase activity, mRNA expression osteoblast differentiation‐associated genes mineralized nodule formation. It also bone morphogenetic proteins‐2, ‐4, ‐7. The...

Emerging evidence indicates the pronounced role of inflammasome activation linked to reactive oxygen species (ROS) in sterile inflammatory response triggered by ischemia/reperfusion (I/R) injury. Ethyl pyruvate (EP) is an antioxidant and conveys myocardial protection against I/R injury, while exact mechanisms remain elusive. We aimed investigate effect EP on injury through related ROS regulation. The rats were randomly assigned four groups: (1) sham, (2) I/R-control (IRC), (3)...

Background: General anesthesia is inevitable for pediatric patients undergoing surgery, though volatile anesthetic agents may cause neuroinflammation and neurodevelopmental impairment; however, the underlying pathophysiology remains unclear. We aimed to investigate mechanism in developing rat brains associated with sevoflurane exposure time, by identifying specific damage-associated molecular patterns (DAMPs) pathway evaluating effects of non-steroidal anti-inflammatory drugs (NSAIDs)...

Hyperglycemia, which reduces the efficacy of treatments and worsens clinical outcomes, is common in stroke. Ability pregabalin to reduce neuroexcitotoxicity may provide protection against stroke, even under hyperglycemia. We investigated its protective effect hyperglycemic stroke possible molecular mechanisms. Male Wistar rats administered dextrose cause hyperglycemia, underwent middle cerebral artery occlusion for 1 h subsequent reperfusion. Rats were treated with an intraperitoneal...

Background Diabetic patients are susceptible to renal ischemia-reperfusion injury, which leads perioperative complications. Activation of NOD-like receptor protein 3 (NLRP3) inflammasome participates in the development diabetes, and contributes injury. Dexmedetomidine (DEX), a highly selective α2-adrenoreceptor agonist, shows renoprotective effects against We aimed elucidate effects, underlying mechanisms, optimal timing DEX treatment diabetic rats. Methods Male Sprague-Dawley rats (n = 12...

Abstract In our previous research showed that tramadol having potential anti-tumor effect was associated with enhancement of oncological prognosis in patients breast cancer surgery. As these effects have not been confirmed by clinical dose-regulated animal or prospective human studies, we investigated the vivo. Female nude mice orthotopically inoculated luciferase-expressing MCF-7 cells, were randomly divided into control (saline), group 1 (1.5 mg kg −1 day ), 2 (3 and morphine (0.5 ) (n =...

Even after recovery from acute kidney injury, glomeruli remain vulnerable to further injury by way of interstitial fibrosis. This study is aimed at elucidating the effects post ischemia-reperfusion (I/R) treatment with trimetazidine on progression renal fibrosis as well short- and intermediate-term aspects. Trimetazidine 3 mg/kg or 0.9% saline was given intraperitoneally once upon reperfusion daily thereafter for 5 d 8 w. Renal histologic changes related signaling proteins were assessed....

Abstract GATA‐4, a zinc finger transcription factor, plays critical role in heart development. Previous studies have shown that p300‐targeted GATA‐4 acetylation increases stability and transcriptional activity, which then stimulates hypertrophy of cardiomyocyte. Erythropoietin (EPO), an essential hypoxia‐induced hormone for normal erythropoiesis, is known to exert cardioprotective effects against disease either ischemic or non‐ischemic origins. Although, various action mechanisms EPO been...