A5023028307- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- DNA Repair Mechanisms
- Plant Genetic and Mutation Studies
- Plant tissue culture and regeneration
- Light effects on plants
- Genomics and Chromatin Dynamics
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Plant Reproductive Biology
- Seed Germination and Physiology
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Plant Virus Research Studies
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Plant responses to water stress
- Transgenic Plants and Applications
- Oil Palm Production and Sustainability
- Immunotherapy and Immune Responses
- Enterobacteriaceae and Cronobacter Research
- Legume Nitrogen Fixing Symbiosis
Institut de Biologie Moléculaire des Plantes
2012-2025
Centre National de la Recherche Scientifique
2010-2025
Université de Strasbourg
2011-2020
Université Clermont Auvergne
2009
Friedrich Miescher Institute
2002-2006
Abstract CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1) is a negative regulator of photomorphogenesis in Arabidopsis thaliana. COP1 functions as an E3 ubiquitin ligase, targeting select proteins for proteasomal degradation plants well mammals. Among its substrates the basic domain/leucine zipper (bZIP) transcription factor ELONGATED HYPOCOTYL5 (HY5), one key regulators under all light qualities, including UV-B responses required tolerance to this environmental threat. Here, we report that, contrast...
Flowering at the right time is crucial to ensure successful plant reproduction and seed yield dependent on both environmental endogenous parameters. Among different pathways that impinge flowering, autonomous pathway promotes floral transition independently of day length through repression central flowering repressor FLOWERING LOCUS C ( FLC ). blocks by repressing integrators such as T FT MSI4/FVE a key regulator reduces expression. Here we report MSI4 protein DDB1 CUL4-associated factor...
In the expanding repertoire of small noncoding RNAs (ncRNAs), tRNA-derived RNA fragments (tRFs) have been identified in all domains life. Their existence plants has already proven but no detailed analysis performed. Here, short tRFs 19–26 nucleotides were retrieved from Arabidopsis thaliana libraries obtained various tissues, submitted to abiotic stress or fractions immunoprecipitated with ARGONAUTE 1 (AGO1). Large differences tRF populations each extract observed. Depending on tRNA, either...
Plants use the energy in sunlight for photosynthesis, but as a consequence are exposed to toxic effect of UV radiation especially on DNA. The UV-induced lesions DNA affect both transcription and replication can also have mutagenic consequences. Here we investigated regulation function recently described CUL4-DDB1-DDB2 E3 ligase maintenance genome integrity upon UV-stress using model plant Arabidopsis. Physiological, biochemical, genetic evidences indicate that this protein complex is...
Homologous recombination (HR) is essential for maintaining genome integrity and variability. To orchestrate HR in the context of chromatin a challenge, both terms DNA accessibility restoration organization after repair. Histone chaperones function nucleosome assembly/disassembly could play role HR. Here, we show that NUCLEOSOME ASSEMBLY PROTEIN1 (NAP1) family histone are required somatic Arabidopsis thaliana. Depletion either NAP1 group or NAP1-RELATED PROTEIN (NRP) proteins caused reduction...
Abstract A genetic screen of a population Arabidopsis thaliana lines exhibiting enhanced somatic homologous recombination yielded mutant affected in expression gene encoding caltractin-like protein (centrin). The hyperrecombinogenic phenotype could be reproduced using RNA interference (RNAi) technology. Both the original and RNAi plants exhibited moderate UV-C sensitivity as well reduced efficiency vitro repair UV-damaged DNA. Transcription profiling showed that components nucleotide...
Significance As obligate photosynthetic and sessile organisms, plants are particularly exposed to the damaging effects of excess light UV wavelengths, which can impact genome integrity by inducing DNA sequence alterations. a response, have evolved efficient surveillance processes, some appear also overlap with mechanisms gene expression control. Our study extends this emerging notion uncovering complex interconnections linking repair RNA silencing in Arabidopsis , illustrating ever-expanding...
Intermolecular recombination events were monitored in Arabidopsis thaliana lines using specially designed traps consisting of tandem disrupted beta-glucuronidase or luciferase reporter genes direct repeat orientation. Recombination frequencies (RFs) varied between the different lines, indicating possible position effects influencing intermolecular processes. The RFs sister chromatids and homologous chromosomes measured plants either hemizygous homozygous for a transgene locus. exceeded those...
Obligate photoautotrophs such as plants must capture energy from sunlight and are therefore exposed to the damaging collateral effects of ultraviolet (UV) irradiation, especially on DNA. Here we investigated interconnection between light signaling DNA repair, two concomitant pathways during photomorphogenesis, developmental transition associated with first exposure. It is shown that combination an enhanced sunscreen effect photoreactivation confers a greater level tolerance UV-C doses in...
In eukaryotes, DNA repair pathways help to maintain genome integrity and epigenomic patterns. However, the factors at nexus of chromatin modification/remodeling remain poorly characterized. Here, we uncover a previously unrecognized interplay between factor DAMAGE BINDING PROTEIN2 (DDB2) methylation machinery in Arabidopsis thaliana Loss-of-function mutation DDB2 leads genome-wide alterations. Genetic biochemical evidence indicate that many repeat loci, influences de novo by interacting with...