A5064038925- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Cell Image Analysis Techniques
- Digital Holography and Microscopy
- Laser Material Processing Techniques
- Image Processing Techniques and Applications
- Orbital Angular Momentum in Optics
- Optical and Acousto-Optic Technologies
- Photoacoustic and Ultrasonic Imaging
- Digital Imaging for Blood Diseases
- Head and Neck Cancer Studies
- Nonlinear Optical Materials Studies
- Bacterial Identification and Susceptibility Testing
- Retinal Development and Disorders
- Botany, Ecology, and Taxonomy Studies
- Botanical Research and Applications
- Ocular and Laser Science Research
- Optical Imaging and Spectroscopy Techniques
- Cervical Cancer and HPV Research
- Cytomegalovirus and herpesvirus research
- Cancer survivorship and care
- Circular RNAs in diseases
- Digital Media Forensic Detection
- Advanced X-ray Imaging Techniques
- Force Microscopy Techniques and Applications
Huazhong University of Science and Technology
2015-2024
Wuhan National Laboratory for Optoelectronics
2016-2024
Wuxi No.2 People's Hospital
2020-2022
People’s Hospital of Rizhao
2021
South China Agricultural University
2017-2019
Compensation of spatial dispersion caused by the acousto-optic deflector (AOD) when using a femtosecond laser is difficult across whole scanning range system, and this significant impediment to its use. In conventional methods, AOD was compensated only it at particular position, while other positions, quality light beam reduced. We developed novel method for compensating within entire special Keplerian telescope. Our experimental results show that residual sufficiently, focal spots reach...
Glioblastoma (GBM) is the most frequent tumor in central nervous system. Long non-coding RNAs (lncRNAs) have been widely accepted as essential participators cancer progression. Nonetheless, specific role and mechanism of lncRNA SRY-box transcription factor 2 overlapping transcript (SOX2-OT) GBM not studied. We evaluated expression levels SOX2-OT, miR-192-5p Ras-related protein Rab-2A (RAB2A) cells via qRT-PCR. To investigate roles SOX2-OT cells, CCK-8, JC-1, EdU, western blot assays were...
Chemotherapy of nasopharyngeal carcinoma (NPC) can lead to significant side effects and complications. Exercises during chemoradiotherapy have potential reduce complications fatigue improve quality life. The aim the randomised clinical study was investigate benefits resistance exercise in NPC patients.A total 146 patients were perform or relaxation exercises chemoradiotherapy. Resistance consisted eight machine-based progressive exercises, control muscle relaxation. Side analysed, assessed...
Background: Concurrent chemo-radiotherapy (CCRT) is an ideal treatment for advanced head and neck squamous cell carcinoma (HNSCC). The performance of CCRT induces severe toxicities in HNSCC patients decreases the quality life (QOL). Maitake D-Fraction proteoglycan which has anti-tumor function associated with its immunomodulatory capacity. polysaccharides also have anti-radiation effect radiation therapy during cancer treatment. This research aimed to illustrate effects on CCRT-associated...
We propose a method to achieve high-resolution femtosecond laser beam shaping (HR-FLBS) via digital holography based on mirror device (DMD) and optimized optical design. By programming the hologram DMD, we captured several patterns such as light spots, decorated letters of "HUST" (Huazhong University Science Technology), series concentric annuli whose fine structure approaches diffraction limit. The experimental results confirm that proposed can shape amplitude phase arbitrarily with high resolution.
The acousto-optic lens (AOL) is becoming a popular tool in the neuroscience field. Here we analyzed deformation of diffraction beam after passage through an AOL consisting pair deflectors using both theoretical and experimental data. results showed that, because high sensitivity optical spatial frequencies deflectors, boundary strength decreases significantly. When focal length diminishes, becomes more serious with smaller size. This finally leads to decreased illuminative numerical...
Abstract Nucleic acids of intact biological tissues are rich in information. Whole‐mount situ hybridization is a powerful technique to mine the wealth data contained DNAs or RNAs, especially mRNAs. However, there no simple, rapid approaches precisely locate mRNAs whole‐mount such as brains. By combining penetration procedures iDISCO with signal amplification approach termed chain reaction, we herein developed method for whole‐brain at cellular resolution. Based on fluorescence tomography...
To resolve fine structures of biological systems like neurons, it is required to realize microscopic imaging with sufficient spatial resolution in three dimensional systems. With regular optical systems, high lateral accessible while axial hard achieve a large volume. We introduce an system for 3D fluorescence volume tissues. Selective plane illumination was adopted provide resolution. A scientific CMOS working sub-array mode kept the area sample surface, which restrained adverse effect...
Label-free imaging technology is a trending way to simplify and improve conventional hematology analysis by bypassing lengthy laborious staining procedures. However, the existing methods do not well balance system complexity, data acquisition efficiency, accuracy, which severely impedes their clinical translation. Here, we propose defocusing phase-contrast under illumination of 415 nm light realize label-free analysis. We have verified that subcellular morphology blood components can be...
The two-photon microscope is a powerful tool in life science. Conventional microscopy can image only plane of particular axial position at time. Axial scanning get the volumetric information, but it gets signals from different positions serially, which means that exposure time every limited. Here we demonstrate novel method, to best our knowledge, simultaneously record images two planes xyz positions. demultiplexing signal realized using confocal strategy. experimental results show be used...
Large aberrations are induced by non-collimated light when the convergence or divergence of incident beam on back-pupil plane objective lens is adjusted for 3D non-inertial scanning. These significantly degrade focus quality and decrease peak intensity femtosecond laser focal spot. Here, we describe an aberration-corrected scanning method lasers based a digital micromirror device (DMD) that used both aberration correction. An imaging setup to detect spot in space, iterative optimization...
Multi-focus parallel scanning can effectively increase laser fabrication throughput. However, the conventional approach of using a spatial light modulator (SLM) to generate multi-foci and scan this fixed number foci with galvanometer scanners only achieve periodic trajectory due low switching speed SLM. Here we demonstrate multi-focus non-periodic method for femtosecond lasers by using, instead, fast-switching digital micromirror device (DMD) dynamic foci. The effective is quickly switched...
<title>Abstract</title> The insufficient coverage of cervical cytology screening in underdeveloped countries or remote areas is currently the bottleneck hurdle to its widespread implementation. Conventional centralized medical methods are heavily dependent on sizable, costly investments as well sufficient qualified pathologists. In this paper, we have developed a precancerous assisted-screening system for identifying high-risk squamous intraepithelial lesion (SIL) cases regions with limited...
Blood analysis through complete blood count is the most basic medical test for disease diagnosis. Conventional requires bulky and expensive laboratory facilities skilled technicians, limiting universal use of outside well-equipped environments. Here, we propose a multiparameter mobile analyzer combined with label-free contrast-enhanced defocusing imaging (CEDI) machine vision instant on-site diagnostic applications. We designed low-cost high-resolution miniature microscope (size: 105 mm × 77...
Digital micromirror devices (DMDs) have shown their potential in 2-photon imaging and microfabrication as diffractive scanners for femtosecond lasers. However, the scanning range of a DMD-based scanner is decreased by spatial filter (SF) used to block undesired diffraction orders. Instead an SF, we present method introducing correcting aberration (ICA) reduce effects these In ICA, aberrations are introduced optical elements, only desired order corrected adding compensatory phase phase. The...
Anti-counterfeiting technique is a research hotspot in information security domain. Common techniques are usually based on artificial physical cloneable feature which forgeable. In this paper, we proposed an anti-counterfeit method microscopic translucent texture of paper (MTTP). MTTP image internal fiber pattern (IFPP) captured by microscope under light source. The IFPP has property uniqueness and randomness, the scale at sub-micron or micron level. Then unable to be forged. Furthermore,...
Blood analysis, through the complete blood count, remains most fundamental medical test for diagnosing broad diseases. Even so, it is still limited to central laboratories with sophisticated facilities and skilled professionals. Here, we propose a simple, machine-vision-aided, label-free analysis technique via regular microscope utilizing contrast-enhance defocus imaging, i.e., defocusing imaging under 415 nm, small aperture illumination. We have shown that this can simultaneously obtain...
Get PDF Email Share with Facebook Tweet This Post on reddit LinkedIn Add to CiteULike Mendeley BibSonomy Citation Copy Text Q. Hu, P. Li, Y. Wang, Xiong, X. Lv, and S. Zeng, "Simultaneous Two-layer Two-photon Imaging Confocal Strategy," in Frontiers Optics / Laser Science, OSA Technical Digest (Optica Publishing Group, 2018), paper JW4A.116. Export BibTex Endnote (RIS) HTML Plain alert Save article
Abstract Laser beam shaping is the process of modulating amplitude and phase a laser beam. With development spatial light modulator, can modulate field to arbitrary pattern dynamically via computer-generated hologram (CGH). Otherwise, femtosecond widely used in material processing owing its high peak power, low hot effect nonlinear effects. However, not strictly monochromatic, which will introduce dispersion when diffracted by deteriorate shaping. Here we present method high-precision...
Abstract Hematology analysis is the most fundamental clinical test in differential diagnosis. The conventional way uses a laboratory hematology analyzer for complete blood count and requires highly trained professionals morphology examination. whole procedure time- labor-intensive, which severely affects disease diagnosis efficiency. Meanwhile, requirement instruments impedes its popularization. Here, we proposed deep-learning assisted, label-free technique through regular microscope to...
Abstract Blood analysis, though complete blood count (CBC), is the most basic medical test for disease diagnosis and health monitoring. However, need bulky expensive laboratory facilities skilled technicians limits universal practices based on analysis outside of well-equipped environments. Here, we proposed a multiparameter mobile analyzer utilizing label-free contrast-enhanced defocusing imaging (CEDI) with assistance machine vision instant on-site analysis. The using CEDI obtains both...