Poria cocos (P. cocos) is an important medicinal fungus in traditional Chinese medicine. acid (PA), a triterpenoid compound, effective component of medicine P. cocos. This experiment investigated the anti-gastric cancer biological activity PA vitro.The effect on viability gastric cells was detected by thiazolyl blue (MTT) assay. Cell adhesion assays were used to detect changes treated after (0, 20, 40, and 80 µmol/L). The ability cell invasion migration Transwell wound healing assays. A...

Abstract Background Gastric cancer (GC) tumorigenesis and treatment failure are caused by stem cells. Polypyrimidine tract binding protein 1 (PTBP1) was shown to be involved in the development of embryonic cells is now being considered as a therapeutic target for tumour progression stem-cell characteristics. Methods PTBP1 expression GC samples detected using tissue microarrays. Proliferation, colony formation, spheroid formation analysis were used examine PTBP1’s role maintenance. In AGS...

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by the hyperproliferation and invasive behavior of rheumatoid fibroblast-like synoviocytes (RA-FLS), which contributes to degradation articular cartilage bone. Inhibition RA-FLS proliferation, migration invasion has become an important therapeutic strategy for RA. Triptolide (TPL), epoxy diterpene lactone compound from traditional Chinese medicine Tripterygium wilfordii Hook. f., significant immunosuppressive...

Atopic dermatitis (AD) is a chronic, recurrent inflammatory skin disorder that causes systemic lesions. The hospital preparation-Qinzhuliangxue mixture (QZLX) has shown potential in alleviating lesions AD patients; however, its underlying mechanisms remain largely unexplored. QZLX's key compounds and their targets were screened by network pharmacology, gene enrichment analysis was performed. Mouse model of induced 2,4-dinitrofluorobenzene (DNFB) established. Then animals divided into control...

New strategies and drugs are urgently needed to improve the treatment of hepatocellular carcinoma (HCC). Vasculogenic mimicry (VM) has been elucidated being associated with progression HCC anti-VM could be a promising strategy. Celastrus orbiculatus extract (COE), mixture 26 compounds isolated from Chinese Herb Orbiculatus Vine, able disrupt VM formation in HCC. This study aims dissect identify potential targets COE on both vitro vivo that distinct our previous study. Proteomics analysis was...

Gastric cancer is a type of malignant tumor that seriously threatens human life and health. Invasion metastasis present difficulties in the treatment gastric cancer, remodeling cytoskeleton plays an important role mediating ability cells to achieve invasion metastasis. Previous experimental results suggest Celastrus orbiculatus extract can regulate cytoskeletal but active component has not been determined. Betulonic acid, as effective COE, inhibits by regulating vitro; its specific...

Objective: To investigate the effect of ethyl acetate extract from Celastrus orbiculatus (COE) on gastric cancer cell apoptosis and reveal its underlying molecular mechanism. In addition, it was aimed to stablish a theoretical basis for clinical application in treatment. Material Methods: Western blot RT-qPCR were used detect mRNA protein expression PHB adjacent tissues. MTT method COE proliferation AGS cells determine 50% inhibitory concentration these cells. evaluated by flow cytometry....

Gastric cancer is the fifth most common tumor and has third-highest mortality rate among various malignant tumors, survival of patients low. Celastrus orbiculatus extract been shown to inhibit activity a variety tumors. This study explored inhibitory effect oleanane-type triterpenoid acid 28-hydroxy-3-oxoolean-12-en-29-oic molecule from on gastric cell invasion metastasis determined its mechanism. 28-Hydroxy-3-oxoolean-12-en-29-oic was first diluted concentrations then used treat SGC-7901...

Abstract Background Polypyrimidine tract binding protein 1 (PTBP1) has been found to play an important role in the occurrence and development of various tumors. At present, PTBP1 gastric cancer (GC) is still unknown worthy further investigation. Methods We used bioinformatics analyze expression patients with GC. Cell proliferation related experiments were detect cell after knockdown. Skeleton staining, scanning electron microscopy transmission observe changes actin skeleton. Proliferation...

Cancer is one of the greatest threats to human health. Gastric cancer (GC) fifth most common malignant tumor in world. Invasion and metastasis are major difficulties treatment GC. Herbal medicines their extracts have a lengthy history being used treat tumors China. The anti-tumoral effects natural products derived from herbs received great deal attention. Our previous studies shown that traditional Chinese herb Celastrus orbiculatus Thunb extract (COE) can inhibit invasion GC cells, but...

Background: Renal cell carcinoma (RCC) is a common cancer of the urinary system. Chinese medicine being extensively used in treatment various cancers. Recent studies found that Poria cocos, widely medicine, has an anti-tumor effect. Pachymic acid (PA) triterpenoid compound P. and present study investigated whether PA main active ingredient this fungus. Methods: The effects on viability, proliferation, adhesion, invasion, metastasis were assessed by...

Background: Gastric cancer is the fifth most common tumor and has third-highest mortality rate among various malignant tumors, survival of patients low. Celastrus orbiculatus extract (COE) been shown to inhibit activity a variety tumors. In this study, we examined inhibition epithelial-mesenchymal transition (EMT) process in gastric cells by COE through transforming growth factor-β (TGF-β) signaling pathway. Methods: was first diluted concentrations then used treat SGC-7901, BGC-823,...

Gastric cancer is a common global disease. So far, the best choice for diagnosis and treatment of gastric includes surgical resection, chemotherapy, other targeted drug therapies; however, overall survival rate patients with still very low. The hypoxic microenvironment facilitates tumor cells to develop tolerance chemotherapy radiotherapy promotes early invasion metastasis various tumors. Celastrus Orbiculatus extract (COE) has shown inhibitory activities against variety cells. In this...

The lymphoma incidence rate is on the rise, with invasive forms particularly prone to relapse following conventional treatment, posing a significant threat human life and wellbeing. Numerous studies have shown that traditional Chinese botanical drug medicine offers promising therapeutic benefits for various malignancies, previous experimental findings indicating Celastrus orbiculatus extract effectively combats digestive tract tumors. However, its impact remains unexplored. This study aims...

Adjuvant chemoradiotherapy, molecular targeted therapy, and immunotherapy are frequently employed to extend the survival of patients with advanced gastric cancer (GC). However, most these treatments have toxic side effects, drug resistance, limited improvements in quality life. Therefore, it is crucial discover develop new medications targeting GC that highly effective minimal toxicity. In previous studies, total terpene extract from stem

Background: This study aimed to determine the effect and mechanism of Xiaoaiping (XAP) injection combined with S-1 in inhibiting invasion metastasis human GC cells. Methods: BGC-823 MGC-803 cells were incubated vitro, effects treatment on cytotoxicity proliferation evaluated by MTT assay. Cell adhesion tests Transwell assays used detect metastatic ability The expression VEGF, Metalloproteinases (MMPs) proteins related Epithelial-Mesenchymal Transition (EMT) detected Western blotting....