A5019100989- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- Circadian rhythm and melatonin
- Genetics, Aging, and Longevity in Model Organisms
- Histone Deacetylase Inhibitors Research
- Autophagy in Disease and Therapy
- Invertebrate Immune Response Mechanisms
- Developmental Biology and Gene Regulation
- Dietary Effects on Health
- Photoreceptor and optogenetics research
- DNA Repair Mechanisms
- Diet and metabolism studies
- Cancer-related Molecular Pathways
- Spaceflight effects on biology
- Light effects on plants
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neurobiology and Insect Physiology Research
- Health and Well-being Studies
- Endoplasmic Reticulum Stress and Disease
- Plant Molecular Biology Research
- Connexins and lens biology
- Genetic Neurodegenerative Diseases
- Tryptophan and brain disorders
- Insect Resistance and Genetics
University of California, Irvine
2021-2022
Kyoto Institute of Technology
2012-2017
The mammalian circadian clock, expressed throughout the brain and body, controls daily metabolic homeostasis. Clock function in peripheral tissues is required, but not sufficient, for this task. Because of lack specialized animal models, it unclear how tissue clocks interact with extrinsic signals to drive molecular oscillations. Here, we isolated interaction between feeding liver clock by reconstituting Bmal1 exclusively hepatocytes (Liver-RE), otherwise clock-less mice, controlling timing...
Life on Earth anticipates recurring 24-hour environmental cycles via genetically encoded molecular clocks active in all mammalian organs. Communication between these controls circadian homeostasis. Intertissue communication is mediated, part, by temporal coordination of metabolism. Here, we characterize the extent to which different organs control systemic metabolic rhythms, an area that remains largely unexplored. We analyzed metabolome serum from mice with tissue-specific expression clock gene
Epigenetics is now emerging as a key regulation in response to various stresses. We herein identified the Drosophila histone methyltransferase G9a (dG9a) factor acquire tolerance starvation stress. The depletion of dG9a led high sensitivity stress adult flies, while its overexpression induced resistance. catalytic domain was not required for plays no apparent role other stresses including heat and oxidative Metabolomic approaches were applied investigate global changes metabolome due loss...
Abstract Circadian rhythms are a foundational aspect of biology. These found at the molecular level in every cell living organism and they play fundamental role homeostasis variety physiological processes. As result, biomedical research circadian continues to expand rapid pace. To support this research, CircadiOmics (http://circadiomics.igb.uci.edu/) is largest annotated repository analytic web server for high-throughput omic (e.g. transcriptomic, metabolomic, proteomic) time series...
Disorganisation and aggregation of proteins containing expanded polyglutamine (polyQ) repeats, or ectopic expression α-synuclein, underlie neurodegenerative diseases including Alzheimer’s, Parkinson, Huntington, Creutzfeldt diseases. Small heat-shock proteins, such as αB-crystallin, act chaperones to prevent protein play a key role in the prevention disorganisation In this study, we have explored potential for chaperone activity αB-crystallin suppress formation aggregates. We tested ability...
Epigenetic regulation in starvation is important but not fully understood yet. Here we identified the Rpd3 gene, a Drosophila homolog of histone deacetylase 1, as critical epigenetic regulator for acquiring stress resistance. Immunostaining analyses fat body revealed that subcellular localization and levels dynamically changed responding to stress. In response stress, level rapidly increased, it accumulated nucleolus what appeared be foci. These observations suggest plays role rRNA synthesis...
Post‐translational modification of the histone plays important roles in epigenetic regulation various biological processes. Among identified methyltransferases ( HMT ases), G9a is a H3 Lys 9 (H3K9)‐specific example active euchromatic regions. Drosophila dG 9a) has been reported to feature H3K9 dimethylation activity vivo . Here, we show that time required for hatching homozygous 9a null mutant and heteroallelic combination mutants delayed, suggesting at least partially responsible...
The gut microbiome influences cognition and behavior in mammals, yet its metabolic impact on the brain is only starting to be defined. Using metabolite profiling of antibiotics-treated mice, we reveal as a key input controlling circadian cycles brain. Intra inter-region analyses characterise influence suprachiasmatic nucleus, containing central clockwork, well hippocampus cortex, regions involved learning behavior.
Abstract Organisms have developed behavioral strategies to defend themselves from starvation stress. Despite of their importance in nature, the underlying mechanisms been poorly understood. Here, we show that Drosophila G9a (dG9a), one histone H3 Lys 9-specific methyltransferases, functions as a key regulator for starvation-induced behaviors. RNA-sequencing analyses utilizing dG9a null mutant flies revealed expression some genes relating gustatory perception are regulated by under...
Endoreplication is a variant type of DNA replication, consisting only alternating G1 and S phases. Many types Drosophila tissues undergo endoreplication. However, the timing extent to which single endocycling macrochaete undergoes temporally programmed endoreplication during development are unclear. Here, we focused on dynamics posterior scutellar (pSC) development. Quantitative analyses C values in shaft cells socket revealed gradual rise from 8C 4C at 8 hours after pupal formation (APF)...
Drosophila Jumonji/Jarid2 (dJmj) has been identified as a component of Polycomb repressive complex 2. However, it is suggested that dJmj both PRC-dependent and –independent roles. Subcellular localization during spermatogenesis unknown. We therefore performed immunocytochemical analyses with specific antibodies to tri-methylation at lysine 27 on histone H3 (H3K27me3). Interestingly, exclusively localizes nucleolus in the late growth stage. Examination various group (PcG) mutant lines stage...
The DNA replication-related element-binding factor (DREF) is a BED finger-type transcription that has important roles in cell cycle progression. In an earlier study, we showed DREF required for endoreplication during posterior scutellar macrochaete development. However, dynamic change the dref expression lineage unclear. this focused on spatio-temporal pattern of gene bristle Gene analysis using GAL4 enhancer trap lines and upstream activation sequence-green fluorescent protein with nuclear...
DREF (DNA replication-related element-binding factor), a zinc finger type transcription factor required for proper cell cycle progression in both mitotic and endocycling cells, is positive regulator of E2F1, an important which regulates genes related to the S-phase cycle. E2F1 regulate similar sets genes, including proliferating nuclear antigen (PCNA), play roles G1 S phase transition. However, relationships between dref e2f1 or PCNA during development are poorly understood. Here, we...
Summary Life on Earth anticipates recurring 24-h environmental cycles via genetically-encoded molecular clocks active in all mammalian organs. Communication between these is believed to control circadian homeostasis. Metabolism can be considered a form of inter- tissue communication language that results temporal coordination systemic metabolism tissues. Here we characterize the extent which different organs employ this means communication, an area remains largely unexplored. For this,...