A5017990584- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- RNA Research and Splicing
- Neurobiology and Insect Physiology Research
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Hippo pathway signaling and YAP/TAZ
- Ubiquitin and proteasome pathways
- CRISPR and Genetic Engineering
- Amyotrophic Lateral Sclerosis Research
- Cancer-related gene regulation
- Developmental Biology and Gene Regulation
- Invertebrate Immune Response Mechanisms
- RNA and protein synthesis mechanisms
- Animal Genetics and Reproduction
- Cancer-related Molecular Pathways
- Genetics, Aging, and Longevity in Model Organisms
- Histone Deacetylase Inhibitors Research
- DNA and Nucleic Acid Chemistry
- Microtubule and mitosis dynamics
- RNA Interference and Gene Delivery
- Hereditary Neurological Disorders
- Molecular Biology Techniques and Applications
- Polyomavirus and related diseases
- Cellular transport and secretion
Kyoto Institute of Technology
2015-2024
GTx (United States)
2018
Hirosaki University
2017
Tokai University
2017
Bio-Medical Science (South Korea)
2016
Universidad Nacional Autónoma de México
2011
Kobe University
1977-2007
Kanagawa Dental University
2003
Aichi Cancer Center
1993-2002
Nagoya University
1988-1999
Many neurodegenerative diseases including Alzheimer, Parkinson, and polyglutamine (polyQ) are thought to be caused by protein misfolding. The polyQ diseases, Huntington disease spinocerebellar ataxias (SCAs), abnormal expansions of the stretch in disease-causing proteins, which trigger misfolding these resulting their deposition as inclusion bodies affected neurons. Although genetic expression molecular chaperones has been shown suppress neurodegeneration, toward developing a therapy, it is...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTExpression of active rat DNA polymerase .beta. in Escherichia coliTakayasu Date, Masamitsu Yamaguchi, Fumiko Hirose, Yoshio Nishimoto, Kiyomi Tanihara, and Akio MatsukageCite this: Biochemistry 1988, 27, 8, 2983–2990Publication Date (Print):April 19, 1988Publication History Published online1 May 2002Published inissue 19 April 1988https://pubs.acs.org/doi/10.1021/bi00408a048https://doi.org/10.1021/bi00408a048research-articleACS PublicationsRequest...
Upstream regions containing a novel common 8-base pair (bp) palindromic sequence, 5'-TATCGATA (Drosophila DNA replication-related element (DRE)), are required for the high expression of Drosophila genes polymerase alpha and proliferating cell nuclear antigen (PCNA) (an auxiliary protein delta). Three DREs one DRE present in gene (nucleotides-217, -83, -30 with respect to transcription initiation site) PCNA (nucleotide-100), respectively. Deletions or 2-bp insertional mutations sequences led...
In the early stage of Drosophila embryogenesis, DNA replication initiates at unspecified sites in chromosome. contrast, specified regions cultured cells. We investigated when and where initiation are during embryogenesis compared them with those observed cells by two-dimensional gel methods. polymerase alpha gene (DNApolalpha) locus, an region, oriDalpha, had been identified Kc cells, repression origin activity coding region was detected after formation cellular blastoderms, range become...
Polyglutamine (polyQ) diseases are a growing class of inherited neurodegenerative including Huntington's disease, which caused by abnormal expansions the polyQ stretch in each unrelated disease protein. The expanded is thought to confer toxic properties on proteins through alteration their conformation leading pathogenic protein–protein interactions oligomerization and/or aggregation. Hypothesizing that molecules with selective binding affinity may interfere properties, we previously...
DREF, a transcription regulatory factor which specifically binds to the promoter-activating element DRE (DNA replication-related element) of DNA genes, was purified homogeneity from nuclear extracts Drosophila Kc cells. cDNA for DREF isolated with reverse-transcriptase polymerase chain reaction method using primers synthesized on basis partial amino acid sequences and following screening libraries. Deduced nucleotide cDNA, is polypeptide 701 residues molecular weight 80,096, contains three...
The genomic and cDNA clones for a Drosophila melanogaster proliferating cell nuclear antigen (PCNA) (cyclin) were isolated sequenced. coding sequence 260-amino-acid residue polypeptide was interrupted by single short intron of 60 base pairs (bp), about 70% the deduced amino acid PCNA identical to rat human polypeptides, with conserved unique repeats leucine in C-terminal region. Genomic Southern blot hybridization analysis indicates presence gene per genome. mRNA detected at high level adult...
We describe a Drosophila gene, orbit, that encodes conserved 165-kD microtubule-associated protein (MAP) with GTP binding motifs. Hypomorphic mutations in orbit lead to maternal effect resulting branched and bent mitotic spindles the syncytial embryo. In larval central nervous system, such mutants have an elevated index some cells showing increase ploidy. Amorphic alleles show late lethality greater frequencies of hyperploid cells. The presence hypomorphic mutant which chromosomes can be...
The abbreviations used are: DNA polymerase, deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase EC 2.7.7.7; SDS, sodium dodecyl sulfate; KP,, potassium phosphate; PC buffer, 50 mM Tris-HCl (pH 7.6). 0.2 m dithiothreitol, 0.1 EDTA, 10% glycerol; DC m~ Tris-HCI 7.6), 1 20% glycerol.
Jarid2 was recently identified as an important component of the mammalian Polycomb repressive complex 2 (PRC2), where it has a major effect on PRC2 recruitment in mouse embryonic stem cells. Although is conserved Drosophila, not previously been implicated (Pc) regulation. Therefore, we purified Drosophila and its associated proteins found that associates with all known canonical components, demonstrating physical interaction flies mammals. Furthermore, vivo studies mutants demonstrate among...
Mutations in the fused sarcoma/translated liposarcoma gene (FUS/TLS, FUS) have been identified sporadic and familial forms of amyotrophic lateral sclerosis (ALS). FUS is an RNA-binding protein that normally localized nucleus, but mislocalized to cytoplasm ALS, comprises cytoplasmic inclusions ALS-affected areas. However, it still unknown whether neurodegeneration occurs ALS caused by loss nuclear function, or gain toxic function due aggregation. Cabeza (Caz) a Drosophila orthologue human...
The relationship between oxidative stress and neurodegenerative diseases has been extensively examined, antioxidants are considered to be a promising approach for decelerating disease progression. Parkinson’s (PD) is common disorder affects 1% of the population over 60 years age. A complex combination genetic environmental factors contributes pathogenesis PD. However, since onset mechanisms PD have not yet elucidated in detail, difficulties associated with developing effective treatments....
Two mRNA species were observed for the<i>Drosophila E2F</i> (<i>dE2F</i>) gene, differing with regard to the first exons (exon 1-a and exon 1-b), which expressed differently during development. A single transcription initiation site containing 1-b was mapped by primer extension analysis numbered +1. We found three tandemly aligned sequences, similar DNA replication-related element (DRE; 5′-TATCGATA), is commonly required of genes related replication cell proliferation, in region upstream...
The genes for a DNA replication-related elementbinding factor (DREF) were isolated from Drosophila melanogaster and virilis, their nucleotide sequences determined. virilis DREF consists of 742 amino acid residues, which is 33 acids longer than D.melanogaster DREF. Comparison the revealed that D.virilis 71% identical to its homolog. Three highly conserved regions identified at positions 14–182 (CR1), 432–568 (CR2) 636–730 (CR3) DREF, with 86.4, 86.1 83.3% identities, respectively. Transgenic...
We have found sequences similar to the transcription factor E2F recognition site within <i>Drosophila</i> proliferating cell nuclear antigen (PCNA) gene promoter. These are located at positions −43 −36 (site I) and −56 −49 II) with respect cap site. Glutathione <i>S</i>-transferase (GST)-E2F GST-DP fusion proteins cooperate bind potential sites in PCNA promoter <i>in vitro</i>. A binding factor(s) these that has specificity of was detected extracts Kc cells. Furthermore, transient expression...
Heat shock transcription factor 1 (HSF1) mediates the induction of heat proteins in response to various types stress. Although HSF1 activity is regulated by its post‐translational modifications, alterations mRNA expression have also been suggested. We here identified three new alternatively spliced isoforms Drosophila HSF (dHSF) mRNA, named dHSFb, dHSFc, and dHSFd. found that ratio dHSFb increases upon exposure, while dHSFd cold exposure. The dHSFc showed greater transcriptional than other...
We have isolated clones containing the entire mouse proliferating cell nuclear antigen (PCNA) gene of 3890 bp and flanking sequences using a rat PCNA cDNA as probe. The has 6 exons whose junction points with introns are extensively homologous to human while sizes nucleotlde much less conserved than exons. By transient expression assay chloramphenicol acetyltransferase, promoter this is localized within 200 upstream transcription initiation site. also two processed pseudogenes. Homology...
Efficient initiation of DNA replication in the absence nonspecific repair synthesis was obtained by using a modification system developed J.J. Li and T.J. Kelly [(1984) Proc. Natl. Acad. Sci. USA 81, 6973-6977]. Circular double-stranded plasmids replicated extracts CV-1 cells only when contained cis-acting origin sequence for simian virus 40 (ori) extract large tumor antigen. Competition between containing ori carrying deletions about served to identify that binds rate-limiting factor(s)...