Introduction: Vascularization remains an obstacle to engineering of larger volume bone tissues. Our aim was induce axial vascularization in a processed bovine cancellous (PBCB) matrix using arteriovenous (AV) loop (artery, vein graft, and vein). Methods: Custom-made PBCB discs (9×5 mm) were implanted into rats. In group A (n= 19), the matrices inserted microsurgically constructed AV loops between femoral vessels graft from contralateral side. B (n = there no vascular carrier. The encased...

Soft-tissue defects of the foot and lower leg caused by traumatic injury, tumor ablation, or infection associated with osteomyelitis often require coverage flaps. One excellent option for reconstruction these is distally based neurofasciocutaneous sural flap. It allows rapid reliable from distal third to forefoot without significant functional donor-site morbidity. However, maximal size flap limited delicate perfusion arterial network superficial sensory nerve. Delay procedures may increase...

Tissue Engineering of skeletal muscle tissue still remains a major challenge. Every neo-tissue construct clinically relevant dimensions is highly dependent on an intrinsic vascularisation overcoming the limitations diffusion conditioned survival. Approaches incorporating arteriovenous-loop model might bring further advances to generation vascularised tissue. In this study 12 syngeneic rats received transplantation carboxy-fluorescine diacetate-succinimidyl ester (CFDA)-labelled, expanded...

Generation of axially vascularized bioartificial bone might be performed using matrix neovascularization in connection with osteoblast injection. We sought to evaluate whether prevascularization porous hard matrices an arteriovenous (AV) loop promotes survival transplanted osteoblasts. A processed bovine cancellous was inserted into the AV loop. Six weeks later, 5 x 10(6) carboxyfluorescein diacetate-stained osteoblasts were injected (group A, n = 34). Osteoblast-seeded without implanted...

Tissue engineering of vascularised skeletal muscle is a promising method for the treatment soft tissue defects in reconstructive surgery. In this study we explored characteristics novel collagen and fibrin matrices engineering. We analyzed newly developed hybrid collagen-I-fibrin-gels nanofibers as well sponges OPLA®-scaffolds. Collagen-fibrin gels were also tested with genipin stabilizing substitute aprotinin. Whereas rapid lysis contraction pure I- or fibrin-matrices have been great...

The modulation of angiogenic processes in matrices is great interest tissue engineering. We assessed the effects fibrin-immobilized VEGF and bFGF an arteriovenous loop (AVL) model 22 AVLs created between femoral artery vein rats. loops were placed isolation chambers embedded 500 µL fibrin gel (FG) (group A) or FG loaded with 0.1 ng/µL B). After two four weeks specimens explanted investigated using histological, morphometrical, ultramorphological [scanning electron microscope (SEM) vascular...

Vascularization still remains an obstacle to engineering of bone tissue with clinically relevant dimensions. Our aim was induce axial vascularization in a large volume approved biphasic calcium phosphate ceramic by transferring the arteriovenous (AV) loop approach animal model. HA/β-TCP granula were mixed fibrin gel for total 16 cm3, followed incorporation into isolation chamber together AV loop. The chambers implanted groins merino sheep and development monitored sequential non-invasive...

Since the implementation and use of silicone implants in breast surgery risks are published discussed. Especially, incidence late implant rupture its potential risk to induce local siliconomas still under discussion not sufficiently evaluated. So far literature data offer no information intrapulmonal or peripheral located cutaneous because systemic migration after augmentation. In light silicones checkered history, given large growing number women who choose undergo augmentation each year,...

We invented an automatic observer-independent quantitative method to analyze vascularization using micro-computed tomography (CT) along with three-dimensional (3D) reconstruction in a tissue engineering model.An arteriovenous loop was created the medial thigh of 30 rats and placed particulated porous hydroxyapatite beta-tricalcium phosphate matrix, filled fibrin (10 mg/mL fibrinogen 2 IU/mL thrombin) without (group A) or B) application fibrin-gel-immobilized angiogenetic growth factors...

The angiogenic effects of different concentrations vascular endothelial growth factor (VEGF) 165 and basic fibroblast (bFGF) immobilized in a fibrin-based drug-delivery system were quantitatively assessed the arteriovenous (AV) loop model. An AV was created medial thigh 60 rats. placed Teflon isolation chamber embedded 500 μL fibrin gel loaded with VEGF bFGF four (no factor, 100 ng/mL VEGF, 25 bFGF, pf bFGF). explantation intervals 1, 2, 4 weeks after initial operation for all groups....

In this study, the authors present a modification of arteriovenous loop model that combines extrinsic and intrinsic vascularization modes to enhance bioartificial matrices.An was created in medial thighs 24 rats. The placed newly developed titanium chamber, which fabricated with an electron beam melting facility, embedded hydroxyapatite/β-tricalcium phosphate/fibrin matrix. At explantation time points (2, 4, 6, 8 weeks), constructs were perfused by differently colored dyes determine amount...

Abstract De novo generation of axially vascularized tissue with clinically relevant dimensions in a large animal model and implementation established imaging modalities for vivo evaluation vascularization. To be used reconstruction defects, engineered grafts need to enable transplantation without graft loss due hypoxia. Limitations small models had not yet been overcome, which is necessary yield clinical relevance. Anatomical studies groin axillary regions eight merino sheep were followed by...

Exposing a vein to altered hemodynamics by creating an arteriovenous (AV) shunt evokes considerable vessel formation that may be of therapeutic potential. However, it is unclear whether the introduction oscillatory flow and/or increase decisive. To distinguish between these mechanical stimuli we grafted femoral into arterial pathway contralateral limb in rats arterioarterial (AA) loop (n = 7). Alternatively, connected artery and using graft, whereby created AV-loop 27). Vessel loops were...

Our aim was to quantitatively assess the angiogenetic effects of VEGF and bFGF immobilized in a fibrin-based drug delivery system suitable subcutaneous rat model. After evaluation implantation technique (6 rats), four teflon isolation chambers containing fibrin gel matrices were implanted subcutaneously an upside-down fashion on back 30 Lewis rats. The consisted 500 microl with two different fibrinogen concentrations (10 mg/ml or 40 fibrinogen) 2 I.U./ml thrombin contained five (0 250 ng/ml...

The purpose of this study was to generate an autonomously vascularized hard-tissue construct suitable for microsurgical transfer. effector vascularization arteriovenous bundle inserted into a specially designed channel in the matrix. authors also evaluated corrosion cast and intravital magnetic resonance angiography as methods monitoring quantifying angiogenic response.Thirty inbred male Lewis rats were divided two groups. In both groups (n = 15), disk processed bovine cancellous bone matrix...

<i>Background/Aims:</i> The aim of this study was to generate an axially vascularized bone substitute. arteriovenous (AV)-loop approach in a large-animal model applied order induce axial vascularization clinically approved processed bovine cancellous (PBCB) matrix significant volume with primary mechanical stability and assess the course increasing vascularization. <i>Methods:</i> PBCB constructs were implanted into 13 merino sheep together microsurgically created AV...