A5077430076- Reconstructive Surgery and Microvascular Techniques
- Wound Healing and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Surgical site infection prevention
- Orthopedic Surgery and Rehabilitation
- Breast Implant and Reconstruction
- Surgical Sutures and Adhesives
- Reconstructive Facial Surgery Techniques
- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- 3D Printing in Biomedical Research
- Angiogenesis and VEGF in Cancer
- Bone fractures and treatments
- Periodontal Regeneration and Treatments
- Body Contouring and Surgery
- Dermatologic Treatments and Research
- Elbow and Forearm Trauma Treatment
- Peripheral Nerve Disorders
- Diabetic Foot Ulcer Assessment and Management
- Dupuytren's Contracture and Treatments
- Trauma Management and Diagnosis
- Cancer Cells and Metastasis
- Peripheral Artery Disease Management
- Dental Implant Techniques and Outcomes
Friedrich-Alexander-Universität Erlangen-Nürnberg
2016-2025
Universitätsklinikum Erlangen
2016-2025
University Hospital Regensburg
2023
University of Regensburg
2023
München Klinik
2023
Creative Commons
2015-2022
Comprehensive Cancer Center Erlangen
2019-2022
Bayer (Germany)
2021
Heidelberg University
2011-2020
Ludwigshafen University of Business and Society
2020
Signal transducer and activator of transcription 3 (STAT3) is phosphorylated by various kinases, several which have been implicated in aberrant fibroblast activation fibrotic diseases including systemic sclerosis (SSc). Here we show that profibrotic signals converge on STAT3 may be an important molecular checkpoint for tissue fibrosis. signaling hyperactivated SSc a TGFβ-dependent manner. Expression profiling functional studies vitro vivo demonstrate mediated the combined action JAK, SRC,...
Introduction: Vascularization remains an obstacle to engineering of larger volume bone tissues. Our aim was induce axial vascularization in a processed bovine cancellous (PBCB) matrix using arteriovenous (AV) loop (artery, vein graft, and vein). Methods: Custom-made PBCB discs (9×5 mm) were implanted into rats. In group A (n= 19), the matrices inserted microsurgically constructed AV loops between femoral vessels graft from contralateral side. B (n = there no vascular carrier. The encased...
Tissue Engineering (TE) and Regenerative Medicine (RM) have gained much popularity because of the tremendous prospects for care patients with tissue organ defects. To overcome common problem donor-site morbidity standard autologous bone grafts, we successfully combined engineering techniques first time arteriovenous loop model to generate vascularized large grafts. We present two cases defects after debridement an osteomyelitis. One was localized in radius one tibia. For osseus...
In addition to good mechanical properties needed for three-dimensional tissue engineering, the combination of alginate dialdehyde, gelatin and nano-scaled bioactive glass (45S5) is supposed combine excellent cellular adhesion, proliferation differentiation properties, biocompatibility predictable degradation rates. The goal this study was evaluate in vitro vivo as a first step on way its use scaffold bone engineering. evaluation showed cell adherence marrow derived mesenchymal stem cells...
Objective Expression of dipeptidylpeptidase 4 ( DPP ‐4) identifies a dermal fibroblast lineage involved in scarring during wound healing. The role DDP ‐4 tissue fibrosis is, however, unknown. aim the present study was to evaluate as potential target for treatment patients with systemic sclerosis SS c). Methods skin biopsy samples and fibroblasts analyzed by real‐time polymerase chain reaction, immunofluorescence, Western blot analyses. activity modulated overexpression, knockdown,...
Bioactive glass (BG) scaffolds are being investigated for bone tissue engineering applications because of their osteoconductive and angiogenic nature. However, to increase the in vivo performance scaffold, including enhancing angiogenetic growth into scaffolds, some researchers use different modifications scaffold addition inorganic ionic components basic BG composition. In this study, we vitro biocompatibility bioactivity Cu2+-doped derived either BMSC (bone-marrow mesenchymal stem...
Volumetric muscle loss caused by trauma or after tumour surgery exceeds the natural regeneration capacity of skeletal muscle. Hence, future goal tissue engineering (TE) is replacement and repair lost newly generating combining different cell sources, such as myoblasts mesenchymal stem cells (MSCs), within a three-dimensional matrix. Latest research showed that seeding on aligned constructs enhance formation myotubes well alignment may provide further step towards clinical application...
Phosphaturic mesenchymal tumor (PMT) is a rare neoplasm of uncertain histogenesis that has been linked to tumor-induced osteomalacia (TIO) since 1959. The neoplastic cells produce increased amount FGF23 which results in TIO via uncontrolled renal loss phosphate (phosphaturia), and consequently diminished bone mineralization. To date, ∼300 cases have reported. Although there increasing evidence PMT can be diagnosed by reproducible histopathologic features, firm diagnosis often restricted...
To overcome common disadvantages of standard cultured epidermal sheet grafts (CEG) we have developed a new technique transplanting human keratinocytes suspended as single cells in fibrin–glue matrix (Keratinocyte–fibrin–glue suspension—KFGS). In an athymic mouse model with reproducible standardized full thickness wounds this was compared directly to CEG. Reepithelialization similar both groups, but reconstitution the dermo-epidermal junction zone, shown by electron microscopy and...
Soft-tissue defects of the foot and lower leg caused by traumatic injury, tumor ablation, or infection associated with osteomyelitis often require coverage flaps. One excellent option for reconstruction these is distally based neurofasciocutaneous sural flap. It allows rapid reliable from distal third to forefoot without significant functional donor-site morbidity. However, maximal size flap limited delicate perfusion arterial network superficial sensory nerve. Delay procedures may increase...
The development of new techniques and modifications to overcome some the disadvantages in cultured keratinocyte grafting has been motivated by several well-known drawbacks use epithelial autografts such as long culture periods, lack adherence, difficulty handling, dermal substrates, high costs. Two recent insights have influenced further research. On one hand, it shown that undifferentiated proliferative cells fibrin glue suspensions is effective reconstitution. other enzymatic release from...
Tissue Engineering of skeletal muscle tissue still remains a major challenge. Every neo-tissue construct clinically relevant dimensions is highly dependent on an intrinsic vascularisation overcoming the limitations diffusion conditioned survival. Approaches incorporating arteriovenous-loop model might bring further advances to generation vascularised tissue. In this study 12 syngeneic rats received transplantation carboxy-fluorescine diacetate-succinimidyl ester (CFDA)-labelled, expanded...
Abstract Background Dupuytren's disease (DD) is a hand disorder mainly among the northern population. In contrast it rare in mediterranean Therefore typical habits and dietetic influences have been discussed as well genetic predisposition. Still, since first description by Dupuytren 1834 only little known about etiology pathogenesis of this disease. Some hints were found for higher prevalence people with diabetes, alcohol abuse or smoking. Also, intensive manual work injuries to an influence...
Generation of axially vascularized bioartificial bone might be performed using matrix neovascularization in connection with osteoblast injection. We sought to evaluate whether prevascularization porous hard matrices an arteriovenous (AV) loop promotes survival transplanted osteoblasts. A processed bovine cancellous was inserted into the AV loop. Six weeks later, 5 x 10(6) carboxyfluorescein diacetate-stained osteoblasts were injected (group A, n = 34). Osteoblast-seeded without implanted...
Tissue engineering of vascularised skeletal muscle is a promising method for the treatment soft tissue defects in reconstructive surgery. In this study we explored characteristics novel collagen and fibrin matrices engineering. We analyzed newly developed hybrid collagen-I-fibrin-gels nanofibers as well sponges OPLA®-scaffolds. Collagen-fibrin gels were also tested with genipin stabilizing substitute aprotinin. Whereas rapid lysis contraction pure I- or fibrin-matrices have been great...
Abstract In many tissue engineering approaches, the basic difference between in vitro and vivo conditions for cells within three‐dimensional (3D) constructs is nutrition flow dynamics. To achieve comparable results , bioreactors are advised improved cell survival, as they able to provide a controlled through scaffold. We hypothesize that bioreactor would enhance long‐term differentiation of osteogenic 3D scaffolds. this either primary rat osteoblasts or bone marrow stromal ( BMSC ) were...