Severe acute respiratory syndrome coronavirus (SARS-CoV) papain-like protease (PLpro) is a key enzyme that plays an important role in SARS virus replication. The ethanol extract of the seeds Psoralea corylifolia showed high activity against SARS-CoV PLpro with IC50 value 15 µg/ml. Due to its potency, subsequent bioactivity-guided fractionation led six aromatic compounds (1–6), which were identified as bavachinin (1), neobavaisoflavone (2), isobavachalcone (3), 4′-O-methylbavachalcone (4),...

High-level expression of recombinant human growth hormone (hGH) in Escherichia coli (E. coli) leads to the formation insoluble aggregates as inclusion bodies devoid biological activity. Until recently, significant efforts have been made improve recovery active hGH from bodies. Here, we developed an efficient procedure for production completely soluble by minimizing and optimizing protein purification conditions. Under newly established conditions were able obtain most total fraction. We show...

Veterinary antibiotics such as sulfonamides are widely used to increase feed efficiency and protect against disease in livestock production. The sulfonamide antimicrobial mechanism involves the blocking of folate biosynthesis by inhibiting bacterial dihydropteroate synthase (DHPS) activity competitively. Interestingly, most treatment can be released into environment via manure result significant diffuse pollution environment. However, physiological effects during plant growth development...

Saponarin (SA), a natural flavonoid, is known for its antioxidant and hepatoprotective activities. SA the predominant compound (1142.7 ± 0.9 mg per 100 g) in barley sprouts, constituting 72% of total polyphenol content. We investigated, first time, effects from sprouts on cellular anti-inflammatory responses. In lipopolysaccharide (LPS)-induced RAW 264.7 macrophages, suppressed activation NF-κB, as evidenced by inhibition NF-κB DNA binding, nuclear translocation, IκBα phosphorylation,...

Mangosenone F (MSF), a natural xanthone, was isolated form Carcinia mangotana , and few studies have reported its glycosidase inhibitor effect. In this study we investigated the anti lung cancer effect of MSF both in vitro vivo . inhibited cell cytotoxicity induced apoptosis via reactive oxygen species (ROS) generation NCI‐H460. treatment also showed pronounced release apoptogenic cytochrome c from mitochondria to cytosol, downregulation Bcl‐2 Bcl‐xL, upregulation Bax, suggesting that...

Salt stress is a major constraint of crop productivity because it reduces yield and limits the expansion agriculture. This study investigated salt tolerance in 26 cultivars cut lilies (Lilium hybrids) by examining effect on growth morphological characteristics flowers leaves their physiological properties (chlorophyll fluorescence). significantly affected development lilies. Canonical discriminant analysis indicates that middle leaf width, number flowers, first flower diameter, petal...

Neuraminidase (NA) is one of the key enzymes responsible for bacterial infection and pathogenesis. This study aimed to gain deeper insights into inhibitory effects flavone-glucosides (1-9) isolated from barley sprouts (BS) on neuraminidase activity. The compounds were identified as, lutonarin (1), saponarin (2), isoorientin (3), orientin (4), isovitexin (5), isoscoparin-7-O-[6-sinapoyl]-glucoside (6), isoscoparin-7-O-[6-feruloyl]-glucoside (7), isovitexin-7-O-[6-sinapoyl]-glucoside (8),...

Rubus coreanus fruit (RF) has been used as a traditional medicine formulation to treat various diseases including diarrhea, asthma, and cancer in East Asia (Korea, China, Japan). RF, which is native Korea, larger size than that of exotic species. In this study, we aimed compare the anti-inflammatory activities immature mature RF extracted with different solvents.Mature (MRF IRF) were 30% ethanol, 70% ethanol water at room temperature. The antioxidant activity was determined using...

Purpose: Aster glehnii (AG) and yomena (AY) are medicinal plants that belong to the family Compositea grow widely in Korea.Plants genus have been used treat snakebite wounds or bruises oriental medicine.This study compared effects of anti-oxidants anti-adipocyte differentiation according species (the aerial parts AG AY).Methods: AY were extracted using 70% ethanol (-E) water (-W) at room temperature.The anti-oxidant activities measured by total phenol contents (TPC), flavonoid (TFC), DPPH...

목적 본 연구의 목적은 생도라지에 비해 증숙 공정을 거친 도라지를 여러 농도의 에탄올로 추출하여 생리활성 효과를 비교 및 조사하여 미백 기능성화장품 소재로서의 활용 가능성을 확인하고자 한다. 방법 B16F10 멜라노마 세포에서 도라지 추출물의 세포독성평가를 확인하기 위하여 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2Htetrazolium (MTS) assay를 수행하였다. 또한, 미백효과를 멜라닌 생합성 함량 측정과 western blot을 통한 생성 관련 인자들의 단백질 발현을 측정하였다. ê²°ê³¼ 추출물은 추출물에 의한 독성을 나타내지 않았다. 측정 결과 70% 에탄올 추출물에서 더 효과적으로 합성을 억제하였다. Tyrosinase-related protein-1 (TRP-1), TRP-2, tyrosinase 또한 ê²°ë¡ 이러한 결과는...

Coreopsis lanceolata L. is a perennial plant of the family Asteraceae, and its flower known to contain flavonoids with various bioactivities. We evaluated effect (CLF) extracts on H2O2-induced oxidative stress (OS) in neuronal cells mouse neurons. The flowering part CL was used as CLF1 (70% ethanol extract) CLF2 (water extract), 10 types phenolic compounds were quantified using high-performance liquid chromatography. To evaluate neuroprotective effects CLF, antioxidant activities measured,...

Scent is one of the most important economic traits in Freesia hybrida. "Shiny Gold", a popular cultivar South Korea, widely cultivated for its scent. The relative scent intensity Gold" was approximately 16% higher full-bloomed flower when compared to yellow bud stage, while tissue-specifically, tepals showed electronic-nose (e-nose) analysis. E-nose analysis also that and lower than "10C3-424" "10C3-894", respectively, similar "Yvonne". These results correlated those olfactory tests. In...

Reactive oxygen species (ROS), associated with oxidative stress, are involved in many biological processes such as apoptosis, necrosis, and autophagy. Oxidative stress might induce neuronal damage via ROS generation, causing neurodegenerative diseases. Erigeron annuus (EA) has antioxidant properties could protect neurons from stress. In this study, we investigated the protective effect of aerial parts (EAA) flowers (EAF) EA on ROS-mediated apoptosis pheochromocytoma 12 cells. We quantified...

Four Korea-native wild vegetables belonging to the Allium genus (Allium tuberosum Rottl., senescens L., thunbergii G. Don., and sacculiferum Maxim.) were investigated for their antioxidant anti-obesity potential, in order promote utilization of edible plants development potential new crops. In this study, we compared activity plant extracts by ABTS+ DPPH scavenging method. We then anti-adipogenic effects adipocytes. quantified phenolics using high-performance liquid chromatography (HPLC)...

External stimulation of the skin by ultraviolet B (UVB) radiation induces oxidative stress or inflammation, causing aging and cancer. Glycyrrhiza uralensis (licorice) has been used as a medicinal plant for its antioxidant, anti-inflammatory, antiviral, antimicrobial, anticarcinogenic, hepatoprotective properties. The present study analyzed effects thermal processing on bioactivities licorice. Heat-treated licorice (HL) extracts had better antioxidant anti-inflammatory activities than...

Background and objective: This study examines the effect of gardening activity level, plant preference, key variables police officers on resilience.Methods: A total 105 male female respondents with an average age 40.6 ± 9.3 were surveyed analyzed.Results: Respondents older age, higher education, monthly income, longer period service tended to show means level. Among three factors resilience (control, sociability, positivity), control was in graduate school group than other groups, showing...

Purpose: Ixeris strigosa (IS) is a perennial plant that commonly grows in meadows.The leaves and roots of IS have been used medicine as sedative.This study evaluated the antioxidant carbohydrate-digestive-enzyme inhibitory effects to determine its potential an essential glycemic inhibitor for type 2 diabetics.Methods: The antioxidative α-amylase α-glucosidase activities were examined using water extracts (ISW), ethanol (ISE), solvent fractions from IS.The measured vitro methods by measuring...

Adipocyte-specific transcription factors and antioxidants are considered the best target of obesity. Aruncus dioicus var. kamtschaticus (A. dioicus, Samnamul) is easily available owing to edible inexpensive. However, anti-adipogenic effects underlying mechanism A. extract (ADE) have not yet been reported. In present study, we evaluate pathway in 3T3-L1 adipocytes, antioxidant activities quantified phenolics using high-performance liquid chromatography ADE. The results revealed ADE had...

Background: Licorice is an important medicinal plant, but climate conditions can hinder seed production. Because it difficult to produce the seeds, Korea mostly depends on imported product. In addition, when grown domestically, standard ingredients do not comply with those of Korean Pharmacopoeia (glycyrrhizin 2.5% and liquritigenin 0.7%). Therefore, necessary develop licorice variety suitable for domestic environment.<BR>Methods Results: A new interspecific hybrid cultivar designated...