Alzheimer's disease (AD) is a progressive neurodegenerative accompanied by irreversible cognitive impairment. A deleterious feedback loop between oxidative stress and neuroinflammation in early AD exacerbates AD-related pathology. Platycodon grandiflorum root extract (PGE) has antioxidant anti-inflammatory effects several organs. However, the mechanisms underlying of PGE brain remain unclear, particularly regarding its impact on oxidative/inflammatory damage Aβ deposition. Thus, we aim to...

In this study, UPLC-QqQ/MS-based lipidomics was applied to profile various lipids from RAW264.7 macrophages treated with different concentrations of lipopolysaccharide (LPS). The degree inflammation increased the LPS concentration. To elucidate altered lipid metabolism inflammatory macrophages, we targeted analyze 25 classes LPS-treated cells. As a result, 523 species were successfully profiled by using optimal UPLC and MRM. Statistical data analyses such as PCA, PLS-DA, HCA differentiated...

Ginseng (Panax ginseng C.A. Mey.) is commonly used in traditional oriental medicine for its wide spectrum of medicinal properties, including anti-inflammatory, antitumorigenic, adaptogenic and anti-aging properties. 20(S)-Protopanaxadiol (PPD), the main intestinal metabolite ginsenosides, one active ingredients ginseng. In this study, we aimed to investigate neuroprotective effects PPD on PC12 cells; however, underlying mechanisms remain elusive. We examined cell viability by MTT assay...

Ginsenoside is the primary active substance of ginseng and has many pharmacological effects, such as anti-cancer, immune, regulating sugar lipid metabolism, antioxidant effects. It also protects nervous cardiovascular systems. This study analyzes effects thermal processing on bioactivities crude saponin. Heat treatment increased contents minor ginsenosides in saponins, Rg3, heat-treated saponin (HGS) had better neuroprotective than non-treated (NGS). HGS reduced glutamate-induced apoptosis...

While both the pro- and anti-inflammatory effects of several eicosanoids have been widely studied, degree inflammation in cells that results from various has yet to be comprehensively studied. The objective this study was assess effect lipopolysaccharide (LPS) treatment on eicosanoid content RAW264.7 cells. An Ultra performance liquid chromatography coupled tandem mass spectrometry (UPLC-MS/MS)-based profiling method used analyze contents treated with different LPS concentrations. data were...

The tuber of Liriope platyphylla Wang et Tang (Liliaceae), also known as Liriopis tuber, is famous in Oriental medicine owing to its tonic, antitussive, expectorant and anti-asthmatic properties. In the present study, effects water extract (LP) on proinflammatory mediators secreted from lipopolysaccharide (LPS)-induced cultured RAW 264.7 mouse macrophages were investigated. Nitric oxide (NO), prostaglandin E2 (PGE2) intracellular calcium release measured after 24 h incubation. Various...

Panax ginseng is a famous traditional medicine in Korea for its beneficial effect on obesity, cardiac and liver associated diseases. The aim of this study was to investigate the metabolite (P. ginseng, Aralicaceae) berries depending ripen stages evaluate potential inhibition adipocyte differentiation 3 T3-L1 cells. Different ripening stage samples P. berry were analyzed through global profiling by NMR spectroscopy. Lipid accumulation cells Oil Red O staining. PLS-DA clearly distinguished...

In this study, we investigated the anti-allergic action of mulberry fruit extract (MFE) or MFE in combination with naringinase (MFEN) IgE-activated RBL-2H3 cells, and mechanisms responsible for effects MFEN. β-hexosaminidase release assay was used to measure amount released from ELISA levels tumor necrosis factor-α (TNF-α). We found that significantly reduced (IC(50), 10.59 mg/ml) TNF-α 4.87 mg/ml). Moreover, MFEN enhanced inhibitory on 123.10 µg/ml) 65.01 µg/ml). Furthermore, had no...

Although Platycodon grandiflorum saponins exhibit many beneficial biological effects in various diseases and conditions, how they protect nerve cells against neurodegenerative Alzheimer's disease (AD) pathology is unknown. We investigated whether P. crude saponin (PGS) protects neurons from neurodegeneration caused by amyloid beta (Aβ)-induced oxidative stress. Hippocampal neuron HT-22 were used the vitro experiment, AD mice (5XFAD mice) as vivo model. Intracellular reactive oxygen species...

Alzheimer’s disease (AD) is a devastating neurodegenerative characterized by irreversible cognitive dysfunction. Amyloid beta (Aβ) peptide an important pathological factor that triggers the progression of AD through accumulation and aggregation, which leads to AD-related pathologies consequently affect functions. Interestingly, several studies have reported Platycodon grandiflorum root extract (PGE), besides exhibiting other bioactive effects, displays neuroprotective,...

Abstract Ginsenoside is the primary active substance of ginseng and has many pharmacological effects, such as anti-cancer, immune- sugar- lipid metabolism-regulating, antioxidant effects. It also protects nervous cardiovascular systems. This study analyzed effects thermal processing on bioactivities crude saponin. Heat treatment increased contents minor ginsenosides in saponin, Rg3, heat-treated saponin (HGS) had better neuroprotective than non-treated (NGS). HGS reduced glutamate-induced...

Rubus coreanus fruit (RF) has been used as a traditional medicine formulation to treat various diseases including diarrhea, asthma, and cancer in East Asia (Korea, China, Japan). RF, which is native Korea, larger size than that of exotic species. In this study, we aimed compare the anti-inflammatory activities immature mature RF extracted with different solvents.Mature (MRF IRF) were 30% ethanol, 70% ethanol water at room temperature. The antioxidant activity was determined using...

Purpose: Aster glehnii (AG) and yomena (AY) are medicinal plants that belong to the family Compositea grow widely in Korea.Plants genus have been used treat snakebite wounds or bruises oriental medicine.This study compared effects of anti-oxidants anti-adipocyte differentiation according species (the aerial parts AG AY).Methods: AY were extracted using 70% ethanol (-E) water (-W) at room temperature.The anti-oxidant activities measured by total phenol contents (TPC), flavonoid (TFC), DPPH...

목적 본 연구의 목적은 생도라지에 비해 증숙 공정을 거친 도라지를 여러 농도의 에탄올로 추출하여 생리활성 효과를 비교 및 조사하여 미백 기능성화장품 소재로서의 활용 가능성을 확인하고자 한다. 방법 B16F10 멜라노마 세포에서 도라지 추출물의 세포독성평가를 확인하기 위하여 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2Htetrazolium (MTS) assay를 수행하였다. 또한, 미백효과를 멜라닌 생합성 함량 측정과 western blot을 통한 생성 관련 인자들의 단백질 발현을 측정하였다. ê²°ê³¼ 추출물은 추출물에 의한 독성을 나타내지 않았다. 측정 결과 70% 에탄올 추출물에서 더 효과적으로 합성을 억제하였다. Tyrosinase-related protein-1 (TRP-1), TRP-2, tyrosinase 또한 ê²°ë¡ 이러한 결과는...

(PG) is used in traditional oriental medicine to treat several ailments.

Coreopsis lanceolata L. is a perennial plant of the family Asteraceae, and its flower known to contain flavonoids with various bioactivities. We evaluated effect (CLF) extracts on H2O2-induced oxidative stress (OS) in neuronal cells mouse neurons. The flowering part CL was used as CLF1 (70% ethanol extract) CLF2 (water extract), 10 types phenolic compounds were quantified using high-performance liquid chromatography. To evaluate neuroprotective effects CLF, antioxidant activities measured,...

Peroxisome proliferator-activated receptor-gamma (PPARgamma) and retinoic acid X-receptor (RXR) heterodimer, which regulates cell growth differentiation, represses the TGFbeta1 gene that encodes for protein involved in cancer biology. This review will introduce novel mechanism associated with inhibition of by PPARgamma activation, dephosphorylation Zf9 transcription factor. Pharmacological manipulation activators can be applied treating TGFbeta1-induced pathophysiologic disorders such as...

Background : A new extraction method-heated ultrasonic was qualitatively and quantitatively analyzed for the of major ginsenosides from ginseng extract; this high-performance liquid chromatography (HPLC) method compared with official Korean industrial standards standard health functional food. Methods Results Ginsenoside compounds were 35 minutes by HPLC analysis using a Halo® RP-Amide column. The validated measurement intra-day inter-day precision, accuracy, limit detection (LOD),...

본 연구는 지방함량이 낮고 건강지향적인 고품질 오디 샐러드드레싱 개발을 위해 식초대신 오미자, 점도를 높이기 생마즙을 사용하여 샐러드 드레싱을 제조하고 그 이화학적 특성 및 항산화성을 분석하였다. 드레싱에 첨가되는 재료는 샐러드베이스(플레인 요거트:마요네에즈:생크림=8:1:1):오디분쇄물:오미자청:생마즙=4:4:6:1 또는 4:4:6:3 으로 첨가하였으며, 이때 첨가된 생마는 전체 드레싱의 0,7, 18% 수준이었다. pH는 생마즙이 증가할수록 높아졌으며, 산도는 낮아졌다. 점도는 첨가하지 않은 대조군은 83.20 cP로 낮았으나, 생마즙 첨가량이 유의적으로 증가하여 7%첨가군은 113 cP, 첨가군은 125 cP 를 나타내었다(p<0.001). 유화 안정성은 첨가량과 관계없이 49-50으로 일정한 값을 나타내었다. 색도 중 명도와 황색도는 높아지는 경향을 총 페놀성 화합물 함량은 대조군이 0.087 mg/g 이었으며, 7% 첨가 드레싱은 0.101 mg/g, 0.122...

Reactive oxygen species (ROS), associated with oxidative stress, are involved in many biological processes such as apoptosis, necrosis, and autophagy. Oxidative stress might induce neuronal damage via ROS generation, causing neurodegenerative diseases. Erigeron annuus (EA) has antioxidant properties could protect neurons from stress. In this study, we investigated the protective effect of aerial parts (EAA) flowers (EAF) EA on ROS-mediated apoptosis pheochromocytoma 12 cells. We quantified...

Four Korea-native wild vegetables belonging to the Allium genus (Allium tuberosum Rottl., senescens L., thunbergii G. Don., and sacculiferum Maxim.) were investigated for their antioxidant anti-obesity potential, in order promote utilization of edible plants development potential new crops. In this study, we compared activity plant extracts by ABTS+ DPPH scavenging method. We then anti-adipogenic effects adipocytes. quantified phenolics using high-performance liquid chromatography (HPLC)...

Background: Glycyrrhiza radix (licorice root) have been used as an oriental medicine material for long time, and its protective effects on oxidative stress, inflammation cognition deficit recently reported. However, the cultivation of species medicinal crops is associated with some problems such low productivity early leaf fall, etc. To resolve this problems, cultivars developed by direct hybridization each Korean researchers. The present study was conducted to compare cultivar (Dagam,...