Bacteria commonly inhabit subsurface oil reservoirs, but almost nothing is known yet about microorganisms that live in naturally occurring terrestrial seeps and natural asphalts are comprised of highly recalcitrant petroleum hydrocarbons. Here we report the first survey microbial diversity ca. 28,000-year-old samples from Rancho La Brea Tar Pits Los Angeles, CA. Microbiological studies included analyses 16S rRNA gene sequences DNA encoding aromatic ring-hydroxylating dioxygenases two tar...

A total of 128 strains was isolated from more than 23 legume hosts in Korea. Phylogenetic relationships between these Korean isolates and reference the genera Bradyrhizobium, Mesorhizobium, Rhizobium Sinorhizobium were analysed using their 16S rRNA gene internally transcribed spacer (ITS) region sequences. Among Bradyrhizobium strains, dendrograms based on both ITS sequences produced two main groups. The tree yielded at least new clusters that discernable seven previously delineated...

Brown algae have been used for their nutritional value as well a source of bioactive compounds with antioxidant, anti-inflammatory, antimicrobial and anti-obesity effects. Obesity is an important condition implicated in various diseases, including diabetes, hypertension, dyslipidemia coronary heart disease. However, effects Eisenia bicyclis remain unknown.

A marine, Gram-negative, aerobic, motile, straight-rod-shaped, moderately halophilic bacterium, designated strain DD-M3 T , was isolated from sea sand in Pohang, Korea. phylogenetic tree based on 16S rRNA gene sequences showed that the fell within evolutionary radiation encompassed by genus Marinobacter . The levels of sequence similarity between novel and type strains recognized species ranged 94.2 to 97.6 %, highest values being with flavimaris SW-145 (97.6 %) lipolyticus SM19 (96.8 %)....

Antibiotic resistance and microbiota within edible snow crabs are important for the Chionoecetes (snow crab) fishing industry. We investigated these parameters using culture methods antibiotic susceptibility tests with six internal organs from three species of Chionoecetes. Each sample revealed many unexpected microbial organs. On basis 16S rRNA sequence analysis 381 isolates, most abundant genera identified in opilio were Acinetobacter spp. (24%), Bacillus (4%), Pseudomonas (34%),...

The Gotjawal areas of Jeju Island, Korea, are comprised unmanaged forests growing on volcanic soils. They support unique assemblages vascular plants from both northern and southern hemispheres, but threatened by human disturbance. health ecosystem function these likely depends in part the diversity community structure soil microbial communities, about which little is known. To assess twenty samples were collected November 2010 4 representatives forests. While properties communities measured...

Three strains, 4M1 T , 4M9 and 4M12, were isolated from cotton waste composts. These strains are Gram-negative, aerobic non-spore-forming rods. 16S rRNA gene sequence comparisons demonstrated that these isolates clustered phylogenetically within the genus Pseudoxanthomonas revealed similarity levels of 96·9–99·0 % to six species with validly published names. According DNA–DNA hybridization, relatedness values between known in range 52–63 %. The DNA G+C content was 66·6–68·4 mol%. For a more...

Abstract The objective of this study was to phylogenetically analyze microorganisms from the domains Bacteria and Archaea in hypersaline sediment Death Valley National Park. Using domain‐specific primers, a region 16S rRNA gene amplified using polymerase chain reaction (PCR), product subsequently used create clone library. A total 243 bacterial clones, 99 archaeal 209 isolates were examined. clones affiliated with following groups: Bacilli (59 clones) Clostridia (1) Firmicutes, Bacteroidetes...

A bacterial strain, GA2-M3 T , isolated from a sea-sand sample in Korea, was subjected to polyphasic taxonomic characterization. Cells of strain were Gram-negative, non-motile, non-spore-forming and short rod- ovoid-shaped. Comparative 16S rRNA gene sequencing studies confirmed that the bacterium fell within radiation genus Loktanella . Similarity levels between sequence those type strains species with validly published names 93.5–96.1 %; highest similarity rosea The G+C content genomic DNA...

A novel bacterial strain, NKM-5(T), was isolated from soil of a lava forest in Nokkome Oreum, Jeju, Republic Korea. Cells strain NKM-5(T) were Gram-stain-positive, motile, endospore-forming, rod-shaped and oxidase- catalase-positive. Strain contained anteiso-C15 : 0 iso-C16 as the major fatty acids; menaquinone-7 (MK-7) predominant isoprenoid quinone; diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysyl-phosphatidylglycerol, an unidentified phospholipid three...

Two Gram-negative, rod-shaped, thermophilic bacterial strains, HC145T and HC148T, were isolated from a compost sample facility in Ichon, Korea. Sequencing of the 16S rRNA genes HC148T comparative analyses resulting sequences clearly showed that these strains had phylogenetic affiliation to genus Ureibacillus. The level similarity between two novel was 98.4 % levels sequence them existing Ureibacillus species 97.8–98.1 (HC145T) 97.4–98.7 (HC148T). DNA–DNA reassociation values type ranged 38...

A novel bacterial strain, designated SA3-7 T , was isolated from soil of a lava forest located in Jeju, Republic Korea. Cells strain were Gram-stain-negative, oxidase- and catalase-positive, non-motile rods produced creamy white colonies on ten-fold-diluted R2A agar. The isolate contained menaquinone-7 (MK-7) as the predominant isoprenoid quinone summed feature 3 (C 16 : 1 ω7 c /C ω6 ), iso-C 15 0 17 3-OH major fatty acids. DNA G+C content 43.1 mol%. Phylogenetic analysis based 16S rRNA gene...

A Gram-stain-negative, non-motile, yellow-pigmented and rod-shaped bacterial strain, designated SN6-13T, was isolated from soil of the Gotjawal, lava forest, located in Jeju, Republic Korea. Cells strain SN6-13T were oxidase- catalase-positive. The isolate contained Q-10 as predominant isoprenoid quinone, summed feature 8 (C18 : 1ω7c and/or C18 1ω6c) C16 0 major fatty acids, sym-homospermidine polyamine diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine,...

A novel bacterial strain designated CB4 T was isolated from soil the Hallasan, Jeju, Korea. Strain found to be strictly aerobic, Gram-stain-positive, rod-shaped, motile and formed creamy greyish colonies on nutrient agar. The major fatty acids were identified as iso-C 15 : 0 16 , predominant isoprenoid quinone MK-7. cell-wall peptidoglycan contained glycine alanine diagnostic amino phosphatidyl- N -methylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol an unidentified...

Using a high-throughput metagenomic approach, we evaluated nifH-harboring bacterial communities and their assembly in the Gotjawal forest, which was naturally formed on basalt rocks with thin layer of soil. Significant differences soil properties community structure were observed comparison similar various habitats, including other lava-formed forests (on Jeju Island Hawaii) regions high humidity (Florida) or low temperatures (Alaska). found to assemble along gradients environmental factors,...

A novel actinobacterial strain, designated N237T, was isolated from sediment soil of wetlands at Meonmulkkak, Dongbaek-Dongsan, the lava forest, Gotjawal, Jeju, Republic Korea. Cells strain N237T were Gram-stain-positive, non-motile rods and formed pale yellow colonies on ten-fold diluted Reasoner's 2A agar. Strain contained iso-C16 : 0 C17 1ω8c as major fatty acids, MK-9(H4) predominant isoprenoid quinone meso-DAP diamino acid in peptidoglycan. It diphosphatidylglycerol,...

The microbial community structure in various microaggregates a loamy sand soil was investigated. were separated into outer and inner aggregates using series of washes. Further physical fractionation achieved by separation coarse fine as macroaggregate fractions, silt microaggregate clay. Research on communities can aid our understanding microhabitats microorganisms structures, with applications that may contribute to increasing crop production maintaining sustainable agriculture. In order...