A5071189984- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- Gene Regulatory Network Analysis
- bioluminescence and chemiluminescence research
- Photochromic and Fluorescence Chemistry
- CRISPR and Genetic Engineering
- Retinal Imaging and Analysis
- Cancer-related gene regulation
- Cardiac pacing and defibrillation studies
- Kruppel-like factors research
- Pluripotent Stem Cells Research
- Cancer-related Molecular Pathways
- Renal and related cancers
- Retinal Diseases and Treatments
- Cancer Genomics and Diagnostics
- Genetic and Kidney Cyst Diseases
- Cardiac electrophysiology and arrhythmias
- Genetic Syndromes and Imprinting
- Circadian rhythm and melatonin
- Nanoplatforms for cancer theranostics
- Hedgehog Signaling Pathway Studies
Masaryk University
2022-2025
Dokuz Eylül University
2024
Izmir University
2020
The complex and heterogeneous nature of hepatocellular carcinoma (HCC) hampers the identification effective therapeutic strategies. Cancer stem cells (CSCs) represent a fraction within tumors with ability to self-renew differentiate, thus significantly contribute formation maintenance tumor mass. Increasing evidence indicates high plasticity in cells, suggesting that non-CSCs could acquire cell properties through de-differentiation or reprogramming processes. In this paper, we reveal KLF4 as...
Diabetic retinopathy (DR) is a leading cause of vision impairment worldwide, driven by chronic hyperglycaemia and its complex metabolic consequences. While animal models have been widely used to study DR, they often fail replicate the physiology human retina. To address this limitation, we employed retinal organoids as model effects across various stages differentiation. Early-stage demonstrated resilience high glucose levels, maintaining normal morphology, viability, gene expression....
Primary cilium projects from cells to provide a communication platform with neighboring and the surrounding environment. This is ensured by selective entry of membrane receptors signaling molecules, producing fine-tuned effective responses extracellular cues. In this study, we focused on one family fibroblast growth factor (FGFRs), their residence within cilia, its role in FGFR signaling. We show that FGFR1 FGFR2, but not FGFR3 FGFR4, localize primary cilia developing mouse tissues vitro...
Cells in the human retina must rapidly adapt to constantly changing visual stimuli. This fast adaptation varying levels and wavelengths of light helps regulate circadian rhythms allows for high illumination, thereby enabling rest system remain responsive. It has been shown that retinal microRNA (miRNA) molecules play a key role regulating these processes. However, despite extensive research using various model organisms, light-regulated miRNAs cells unknown. Here, we aim characterize miRNAs....
Luciferase reporter assays represent a simple and sensitive experimental system in cell molecular biology to study multiple biological processes. However, the application of these is often limited by costs conventional luminometer instruments versatility their use different conditions. Therefore, we aimed develop small, affordable allowing continuous measurement luciferase activity, designed for inclusion into various kinds tissue culture incubators. Here, introduce LuminoCell—an open-source...
Primary cilia are cellular surface projections enriched in receptors and signaling molecules, acting as hubs that respond to stimuli. Malfunctions primary have been linked human diseases, including retinopathies ocular defects. Here, we focus on TMEM107, a protein localized the transition zone of cilia. TMEM107 mutations were found patients with Joubert Meckel–Gruber syndromes. A mouse model lacking Tmem107 exhibited eye defects such anophthalmia microphthalmia, affecting retina...
Abstract Hepatic fibrogenesis is a pathological outcome of chronic liver injury, characterized by the excessive accumulation extracellular matrix proteins, leading to hepatocarcinogenesis. However, lack reliable models that mimic precancerous in early stage disease remains significant obstacle. Here, we utilized human pluripotent stem cell-derived hepatic organoids (eHEPO) replicate stages fibrosis, focusing on CRISPR/Cas9 system-mediated TP53 loss within pro-carcinogenic microenvironment...
Human induced pluripotent stem cell (iPSC) lines were generated from peripheral blood mononuclear cells (PBMCs) isolated two related patients diagnosed with either idiopathic ventricular fibrillation or catecholaminergic polymorphic tachycardia, carrying an unknown variant in the RYR2 gene, c.14201A>G (p.Y4734C) and one healthy individual. Reprogramming was done using a commercially available Epi5 Kit. The pluripotency of iPSC verified by expression markers their capacity to differentiate...
Abstract Retinal microRNA (miRNA) molecules play critical roles in a wide range of processes including cell proliferation, death, and synaptic plasticity. Recently they have been shown to regulate crucial that are associated with perception light visual function, adaptation, control genes regulating circadian entrainment. Despite extensive work on retinal miRNAs different model organisms, light-regulated human retina not known. Here, we aim characterize these miRNAs. We generated responsive...
Cells in the human retina must rapidly adapt to constantly changing visual stimuli. This fast adaptation varying levels and wavelengths of light helps regulate circadian rhythms allows for high illumination, thereby enabling rest system remain responsive. It has been shown that retinal microRNA (miRNA) molecules play a key role regulating these processes. However, despite extensive research using various model organisms, light-regulated miRNAs cells unknown. Here, we aim characterize miRNAs....
Abstract Luciferase reporter assays represent a simple and sensitive experimental system in cell molecular biology to study multiple biological processes. However, the application of these is often limited by costs conventional luminometer instruments versatility their use different conditions. Therefore, we aimed develop small, affordable allowing continuous measurement luciferase activity, designed for inclusion into various kinds tissue culture incubators. Here introduce LuminoCell - an...