A5062944273- Liver physiology and pathology
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Research in Cotton Cultivation
- Lipid metabolism and biosynthesis
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Kruppel-like factors research
- Pharmacogenetics and Drug Metabolism
- Cancer-related Molecular Pathways
- Pancreatic function and diabetes
- Cancer-related gene regulation
- Genetic Syndromes and Imprinting
- RNA Research and Splicing
- Planarian Biology and Electrostimulation
- Antioxidant Activity and Oxidative Stress
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Wnt/β-catenin signaling in development and cancer
Dokuz Eylül University
2017-2024
Izmir University
2019-2023
Organoid technologies have become a powerful emerging tool to model liver diseases, for drug screening, and personalized treatments. These applications are, however, limited in their capacity generate functional hepatocytes reproducible efficient manner. Here, we generated characterized the hepatic organoid (eHEPO) culture system using human induced pluripotent stem cell (iPSC)-derived EpCAM-positive endodermal cells as an intermediate. eHEPOs can be produced within 2 weeks expanded long...
The bioengineerined and whole matured human brain organoids stand as highly valuable three-dimensional in vitro brain-mimetic models to recapitulate vivo development, neurodevelopmental neurodegenerative diseases. Various instructive signals affecting multiple biological processes including morphogenesis, developmental stages, cell fate transitions, migration, stem function immune responses have been employed for generation of physiologically functional cerebral organoids. However, the...
The complex and heterogeneous nature of hepatocellular carcinoma (HCC) hampers the identification effective therapeutic strategies. Cancer stem cells (CSCs) represent a fraction within tumors with ability to self-renew differentiate, thus significantly contribute formation maintenance tumor mass. Increasing evidence indicates high plasticity in cells, suggesting that non-CSCs could acquire cell properties through de-differentiation or reprogramming processes. In this paper, we reveal KLF4 as...
The hepatotoxicity of drugs is one the leading causes drug withdrawal from pharmaceutical market and high attrition rates. Currently, commonly used hepatocyte models include conventional hepatic cell lines animal models, which cannot mimic human drug-induced liver injury (DILI) due to poorly defined dose-response relationships and/or lack human-specific mechanisms toxicity. In comparison 2D culture systems different sources such as primary hepatocytes hepatomas,, 3D organoids derived an...
The variations in perilipin gene (PLIN) were previously associated with obesity. We examined the association of polymorphisms at PLIN locus adolescents obesity and their connection serum adipokines. A total 308 children (206 obese, 66.8% 102 healthy control, 33.2%) between ages 10-18 years included into study. analysis [PLIN 1, 4, 6, 5'UTR-1234 C > G 10171 A/T] studied by Real Time-PCR. Serum leptin, adiponectin, resistin ghrelin levels ELISA method both groups link analyzed. leptin level...
The generation and use of induced pluripotent stem cells (iPSCs) in order to obtain all differentiated adult cell morphologies without requiring embryonic is one the most important discoveries molecular biology. Among uses iPSCs neuron organoids study biological cues underlying neuronal brain development, addition neurological diseases. These iPSC-derived differentiation models allow us examine gene regulatory factors involved such processes. these are long non-coding RNAs (lncRNAs), genes...
Abstract Hepatic fibrogenesis is a pathological outcome of chronic liver injury, characterized by the excessive accumulation extracellular matrix proteins, leading to hepatocarcinogenesis. However, lack reliable models that mimic precancerous in early stage disease remains significant obstacle. Here, we utilized human pluripotent stem cell-derived hepatic organoids (eHEPO) replicate stages fibrosis, focusing on CRISPR/Cas9 system-mediated TP53 loss within pro-carcinogenic microenvironment...