A5088811460- Liver physiology and pathology
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- Hepatocellular Carcinoma Treatment and Prognosis
- Redox biology and oxidative stress
- Cancer, Hypoxia, and Metabolism
- Pluripotent Stem Cells Research
- Telomeres, Telomerase, and Senescence
- Proteoglycans and glycosaminoglycans research
- Cancer, Lipids, and Metabolism
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Liver Disease Diagnosis and Treatment
- Ocular Oncology and Treatments
- Lipid metabolism and biosynthesis
- Cancer Mechanisms and Therapy
- Plant and Fungal Interactions Research
- Hemispheric Asymmetry in Neuroscience
- Nuts composition and effects
- Genetics and Physical Performance
- Corneal surgery and disorders
- Fibroblast Growth Factor Research
Dokuz Eylül University
2014-2025
Pediatrics and Genetics
2024
Izmir University
2019-2023
Ankara Yıldırım Beyazıt University
2023
Institute for Biomedicine
2017
Adnan Menderes University
2015
Bilkent University
2005-2009
beta-catenin mutations that constitutively activate the canonical Wnt signaling have been observed in a subset of hepatocellular carcinomas (HCCs). These are associated with chromosomal stability, low histological grade, tumor invasion and better patient survival. We hypothesized is selectively activated well-differentiated, but repressed poorly differentiated HCCs. To this aim, we characterized differentiation status HCC cell lines compared their expression pathway genes, explored activity...
Organoid technologies have become a powerful emerging tool to model liver diseases, for drug screening, and personalized treatments. These applications are, however, limited in their capacity generate functional hepatocytes reproducible efficient manner. Here, we generated characterized the hepatic organoid (eHEPO) culture system using human induced pluripotent stem cell (iPSC)-derived EpCAM-positive endodermal cells as an intermediate. eHEPOs can be produced within 2 weeks expanded long...
Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer and third leading cause cancer-related deaths worldwide. Limitations in HCC treatment result due to poor prognosis resistance against traditional radiotherapy chemotherapies. The multikinase inhibitor sorafenib only FDA approved drug available for advanced patients, development second-line options patients who cannot tolerate or develop an urgent medical need. In this study, we established sorafenib-resistant...
<b><i>Objective:</i></b> Levels of serum albumin have recently emerged, together with C-reactive protein, as an important prognostic indicator for hepatocellular carcinoma (HCC). It has been reported that larger HCCs are associated lower levels. However, the albumin-mediated growth decrease yet to be determined. <b><i>Methods:</i></b> We examined a large HCC cohort and then by direct exposure cells in vitro, relationship levels growth....
The therapeutic induction of senescence is a potential means to treat cancer, primarily acting through the persistent growth-arrested state in tumors. However, recent studies have indicated that therapy-induced (TIS) tumor cells allows for prolonged survival subgroup dormant state, with re-enter cell cycle along an increased stemness gene expression. Residual after TIS cancer stem phenotype may profound implications aggressiveness and disease recurrence. Herein, we investigated...
The bioengineerined and whole matured human brain organoids stand as highly valuable three-dimensional in vitro brain-mimetic models to recapitulate vivo development, neurodevelopmental neurodegenerative diseases. Various instructive signals affecting multiple biological processes including morphogenesis, developmental stages, cell fate transitions, migration, stem function immune responses have been employed for generation of physiologically functional cerebral organoids. However, the...
Caveolin-1 is the main component of caveolae membrane structures and has different roles during tumorigenesis in cancer types with varying expression profiles, indicating that role caveolin-1 varies according to tumor type. In this study, we investigated hepatocellular carcinogenesis.We analyzed 96 carcinoma (HCC), 29 cirrhosis, 20 normal liver tissues 9 HCC cell lines by immunostaining western blotting, respectively. After was stably transfected HepG2 Huh7 cells, effects on cellular...
Abstract Background Hepatocyte growth factor (HGF) induced c-Met activation is known as the main stimulus for hepatocyte proliferation and essential liver development regeneration. Activation of HGF/c-Met signaling has been correlated with aggressive phenotype poor prognosis in hepatocellular carcinoma (HCC). MUC1 a transmembrane mucin, whose over-expression reported most cancers. Many oncogenic effects are believed to occur through interaction molecules. To clarify role signaling, we...
Senescence is a permanent proliferation arrest in response to cell stress such as DNA damage. It contributes strongly tissue aging and serves major barrier against tumor development. Most cells are believed bypass the senescence (become "immortal") by inactivating growth control genes TP53 CDKN2A. They also reactivate telomerase reverse transcriptase. Senescence-to-immortality transition accompanied phenotypic biochemical changes mediated genome-wide transcriptional modifications. This...
The complex and heterogeneous nature of hepatocellular carcinoma (HCC) hampers the identification effective therapeutic strategies. Cancer stem cells (CSCs) represent a fraction within tumors with ability to self-renew differentiate, thus significantly contribute formation maintenance tumor mass. Increasing evidence indicates high plasticity in cells, suggesting that non-CSCs could acquire cell properties through de-differentiation or reprogramming processes. In this paper, we reveal KLF4 as...
Graphical Abstract Wnt and TGF-β pathways mediate the regorafenib resistance.
BAG-1 interacts with multiple partners, particularly c-Raf, and promotes cancer cell survival. Hence, modulating the BAG-1-associated interactions novel inhibitors could provide benefit for therapy. Using HDX-MS, we first demonstrate higher-order structure of BAG-1S identify a potential "druggable" site on its BAG domain. An LC-MS/MS-coupled cell-free binding experiment is then used to map BAG-1S:c-Raf interface, uncovering 20-amino acid-length region that most likely interact c-Raf....
Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related mortality worldwide. Deregulation AKT signaling pathway has been found in HCC. However, effect activation on proliferation and apoptosis HCC not clear. Herein, expression phosphorylated form (Ser 473) was investigated tumor (n=73), cirrhosis (n=17), normal liver (n=22) samples cell lines (n=8). The results showed that p-AKT higher (53%) than cirrhotic tissues (12%) while it absent (p<0.0001). also associated with...
Abstract We studied in vitro effects of glycogen synthase kinase 3β (GSK3β)‐inhibitor lithium on the growth hepatocellular carcinoma (HCC) cells. Lithium induced strong inhibition (>70%) 75% ( n = 9 12) cell lines, apparently independent from status major genes that are mutated HCC including p53 , p16 INK4a β‐ catenin and Axin1 . Comparative studies with a growth‐sensitive Huh7 growth‐resistant Hep40 lines showed induces arrest cells but not accumulation N‐terminally phosphorylated...
Tumor cells have the capacity to proliferate indefinitely that is qualified as replicative immortality. This ability contrasts with intrinsic control of number cell divisions in human somatic tissues by a mechanism called senescence. Replicative immortality acquired inactivation p53 and p16 INK4a genes reactivation hTERT gene expression. It unknown whether cancer reversible. Here, we show spontaneous induction senescence p53-and -deficient hepatocellular carcinoma cells. phenomenon...
The Hepatocyte Growth Factor (HGF)/c-Met signaling pathway regulates hepatocyte proliferation, and aberrations are implicated in the invasive metastatic behaviors of hepatocellular carcinoma (HCC). In addition to c-Met, heparin acts as a co-receptor modulate activity. Recently, anti-metastatic anti-cancer effects have been reported. However, role regulation HGF remains controversial on HGF-induced biological responses during hepatocarcinogenesis is not yet defined. this study we determined...
Neural regeneration research is designed in part to develop strategies for therapy after nerve damage due injury or disease. In this study, a new gelatine-based biomimetic scaffold was fabricated brain tissue engineering applications. A technique combining thermally induced phase separation and porogen leaching used create interconnected macropores nanofibrous structure. To promote processes, the scaffolds were integrated with growth factor (NGF)-loaded alginate microspheres. The results...
c-Met, the receptor for Hepatocyte Growth Factor (HGF), overexpressed and deregulated in Hepatocellular Carcinoma (HCC). Caveolin 1 (CAV1), a plasma membrane protein that modulates signal transduction molecules, is also HCC. The aim of this study was to investigate biological clinical significance co-expression activation c-Met CAV1 We showed were co-localized HCC cells HGF treatment increased association. HGF-triggered caused concurrent rise both phosphorylation expression CAV1. Ectopic...
Purpose To develop a high‐resolution three‐dimensional (3D) magnetic resonance imaging (MRI)‐based treatment planning approach for uveal melanomas (UM) in proton therapy. Materials/methods For eight patients with UM, segmentation of the gross tumor volume (GTV) and organs‐at‐risk (OARs) was performed on T1‐ T2‐weighted 7 Tesla MRI image data to reconstruct patient MR‐eye. An extended contour defined 2.5‐mm isotropic margin derived from GTV. A broad beam algorithm, which we have called πDose,...
Correlative microscopy is a sophisticated imaging technique that combines optical and electron microscopes, with the most common approach being integration of light microscopy, known as correlative (CLEM). While CLEM provides comprehensive view biological samples, it presents significant challenge in sample preparation due to distinct processes involved each technique. Striking balance between these methods crucial. Despite numerous approaches, achieving seamless remains complex task....