A5042358406- Mitochondrial Function and Pathology
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Metabolism and Genetic Disorders
- Peroxisome Proliferator-Activated Receptors
- Liver Disease Diagnosis and Treatment
- Lipid metabolism and biosynthesis
- Advanced Chemical Sensor Technologies
- Birth, Development, and Health
- ATP Synthase and ATPases Research
- Adipose Tissue and Metabolism
- Fatty Acid Research and Health
- Genomics, phytochemicals, and oxidative stress
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Selenium in Biological Systems
- Diet and metabolism studies
- Reproductive Health and Technologies
- Diet, Metabolism, and Disease
- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Genetics, Aging, and Longevity in Model Organisms
- Photovoltaic Systems and Sustainability
- Electrochemical sensors and biosensors
University of Salento
2016-2025
Center for Biomolecular Nanotechnologies
2017-2018
Italian Institute of Technology
2017-2018
CoNISMa
2014
Istituto Nazionale di Fisica Nucleare, Sezione di Lecce
2000-2006
Mitochondrial dysfunction is a key feature of cancer and frequently associated with increased aggressiveness metastatic potential. Recent evidence has brought to light metabolic rewiring that takes place during the epithelial-to-mesenchymal transition (EMT), process drives invasive capability malignant tumors, highlights mechanistic link between mitochondrial EMT been only partially investigated. In this study, we characterized function bioenergetic status cultured human breast cell lines,...
Krill oil (KO) is a dietary source of n-3 polyunsaturated fatty acids, mainly represented by eicosapentaenoic acid and docosahexaenoic bound to phospholipids. The supplementation high-fat diet with 2.5% KO efficiently prevented triglyceride cholesterol accumulation in liver treated rats. This effect was accompanied parallel reduction the plasma levels triglycerides glucose prevention insulin increase. investigation molecular mechanisms action fed animals revealed strong decrease activities...
Summary Recent evidences have linked abdominal obesity, insulin resistance, and dyslipidemia to male infertility. Since a defective energy metabolism may play an important role in the impairment of sperm quality, aim this study is investigate energetic rats fed with high‐fat diet, animal model associated metabolic syndrome development. Sexually mature Sprague–Dawley were divided into two groups for 4 weeks standard diet (control group) or enriched 35% fat ( high group). Liver adipose tissue...
Differently from higher eukaryotic cells, in the yeast Saccharomyces cerevisiae there are two mitochondrial carrier proteins involved transport of citrate: Ctp1 and Yhm2. Very little is known about physiological role these proteins. Wild-type mutant strains deleted CTP1 YHM2 were grown media supplemented with a fermentable (glucose) or nonfermentable (ethanol) carbon source. To assess changes Yhm2 mRNA expression levels, real-time PCR was performed after total RNA extraction. In wild-type...
Summary Recently, obesity has been linked to male infertility. In animal models the administration of a high‐fat diet caused reduction in sperm quality, by impairing gamete energy metabolism. The aim this study was investigate possible effect dietary fatty acids supplementation modulation metabolism and, turn, improvement quality rats fed diet. Sexually mature Sprague–Dawley were divided into four groups and for 4 weeks standard (control group), (enriched 35% fat 15% sucrose), supplemented...
Sperm cells progressive motility is the most important parameter involved in fertilization process. middle piece contains mitochondria, which play a critical role energy production and whose proper operation ensures reproductive success. Notably, sperm strictly related to mitochondrial membrane potential (MMP) consequently functionality. Although previous studies presented an evaluation of function through MMP assessment entire samples, quantitative approach at single-cell level could...
Sperm motility is the most important parameter involved in fertilization process and it strictly required for reproductive success. Although sperm movements are essential physiologic process, data, deriving from studies focused on research of altered cell pathways asthenozoospermia, offer only limited information about molecular mechanism underlying motility. The aim this study was to identify proteins human deficiency by using label-free mass-spectrometry liquid chromatography (LC-MS/MS)....
In this work we report a relatively simple and fast method for analysing oxygen consumption therefore mitochondrial functionality, in individual human ejaculates. This oxygraphic requires low number of cells, is highly reproducible linearly correlates with sperm concentration. Our results have shown that uptake by mitochondria demembranated cells from normozoospermic subjects significantly stimulated large set respiratory substrates ADP. The control ratio (RCR) values indicate good coupling...
Metabolite carrier proteins of the mitochondrial inner membrane share homology in their transmembrane domains, which also carries targeting information. In addition, some carriers have cleavable presequences are not essential for targeting, but other function before import. The cytosolic chaperones Hsc70 (heat-shock cognate 70) and Hsp90 protein 90) complex with precursors interact specifically Tom (translocase outer membrane) 70 import receptor to promote We analysed how PiC (phosphate...