A5064726232- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Neurobiology and Insect Physiology Research
- Digital Holography and Microscopy
- Optical Coherence Tomography Applications
- Computational Drug Discovery Methods
- Neuroscience and Neural Engineering
- Cell Image Analysis Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Neural Networks and Applications
- Advanced X-ray Imaging Techniques
- Retinal Development and Disorders
- Visual perception and processing mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Prenatal Substance Exposure Effects
- Olfactory and Sensory Function Studies
- EEG and Brain-Computer Interfaces
- Image Processing Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
University of Minnesota
2024
Janelia Research Campus
2014-2019
Howard Hughes Medical Institute
2014-2019
Helix (United States)
2014
Harvard University
2010-2011
Albany Medical Center Hospital
2003-2008
The active properties of dendrites can support local nonlinear operations, but previous imaging and electrophysiological measurements have produced conflicting views regarding the prevalence selectivity nonlinearities in vivo. We imaged calcium signals pyramidal cell motor cortex mice performing a tactile decision task. A custom microscope allowed us to image soma up 300 μm contiguous dendrite at 15 Hz, while resolving individual spines. New analysis methods were used estimate frequency...
In traditional zonal wavefront sensing for adaptive optics, after local gradients are obtained, the entire can be calculated by assuming that is a continuous surface. Such an approach will lead to sub-optimal performance in reconstructing wavefronts which either discontinuous or undersampled sensor. Here, we report new method reconstruct directly measuring phases parallel using multidither coherent optical technique. This determines relative of each pupil segment independently, and thus...
Summary The active properties of dendrites support local nonlinear operations, but previous imaging and electrophysiological measurements have produced conflicting views regarding the prevalence nonlinearities in vivo . We imaged calcium signals pyramidal cell motor cortex mice performing a tactile decision task. A custom microscope allowed us to image soma up 300 μm contiguous dendrite at 15 Hz, while resolving individual spines. New analysis methods were used estimate frequency spatial...
Neurons and neural networks often extend hundreds to thousands of micrometers in three dimensions. To capture all the calcium transients associated with their activity, we need volume imaging methods sub-second temporal resolution. Such speed is challenging for conventional two-photon laser scanning microscopy (2PLSM) achieve, because its dependence on serial focal 3D limited brightness indicators. Here present an optical module that can be easily integrated into standard 2PLSMs generate...
SignificanceThe development of genetically encoded fluorescent indicators neural activity with millisecond dynamics has generated demand for ever faster two-photon (2P) imaging systems, but acoustic and mechanical beam scanning technologies are approaching fundamental limits. We demonstrate that potassium tantalate niobate (KTN) electro-optical deflectors (EODs), which not subject to the same limits, capable ultrafast two-dimensional (2D) 2P in vivo.AimTo determine if KTN-EODs suitable...