A5055485907- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Fungal Biology and Applications
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Protein Kinase Regulation and GTPase Signaling
- RNA modifications and cancer
- Metal complexes synthesis and properties
- Nitric Oxide and Endothelin Effects
- Bioactive Compounds and Antitumor Agents
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Phytochemistry and Biological Activities
- Lung Cancer Research Studies
- Lung Cancer Treatments and Mutations
- Sesquiterpenes and Asteraceae Studies
- Bacterial biofilms and quorum sensing
- Cancer Cells and Metastasis
- Cancer therapeutics and mechanisms
- DNA Repair Mechanisms
- Phytochemistry and Bioactivity Studies
- Antibiotic Resistance in Bacteria
- Sirtuins and Resveratrol in Medicine
- Enzyme function and inhibition
National Taiwan University
2005-2021
National Taiwan University Hospital
1995-2010
National Chiayi University
2002-2009
Academia Sinica
2009
National Cheng Kung University
2002
Chinese Culture University
2000
Institute of Chemistry, Academia Sinica
2000
Scripps Research Institute
2000
Chung Hwa University of Medical Technology
2000
National Taiwan University of Science and Technology
1998
Resveratrol (3,5,4-trihydroxy- trans -stilbene) is a phytoalexin compound with anti-inflammatory and antioxidant activities. The effect of resveratrol on swarming virulence factor expression Proteus mirabilis , an important pathogen infecting the urinary tract, was determined agar plates without compound. Bacteria harvested at different times were assayed for cell length production flagella, haemolysin urease. inhibited P. in dose-dependent manner. significantly 15 μg ml −1 completely 60 ....
The signaling pathway involved in protein kinase C (PKC) activation and role of PKC isoforms lipopolysaccharide (LPS)-induced nitric oxide (NO) release were studied primary cerebellar astrocytes. LPS caused a dose- time-dependent increase NO inducible synthase (iNOS) expression. tyrosine inhibitor, genestein, the phosphatidylcholine-phospholipase D609, phosphatidate phosphodrolase propranolol, attenuated effects, whereas PI-PLC U73122, had no effect. inhibitors (staurosporine, Ro 31–8220, Go...
Abstract The signaling pathway for protein kinase C (PKC) activation and the role of PKC isoforms in LPS-induced nitric oxide (NO) release were studied RAW 264.7 macrophages. tyrosine inhibitor genestein attenuated NO inducible synthase (iNOS) expression, as did phosphoinositide-specific phospholipase (PI-PLC) U73122 phosphatidylcholine-specific (PC-PLC) D609. LPS stimulated phosphatidylinositol (PI) hydrolysis activity cells; both inhibited by genestein. inhibitors (staurosporine,...
The stilbenoids, arachidin-1 (Ara-1), arachidin-3, isopentadienylresveratrol, and resveratrol, have been isolated from germinating peanut kernels characterized as antioxidant anti-inflammatory agents. Resveratrol possesses anticancer activity, studies indicated that it induces programmed cell death (PCD) in human leukemia HL-60 cells. In this study, the activity of these stilbenoids was determined Ara-1 had highest efficacy inducing PCD cells, with an approximately 4-fold lower EC50 than...
The alkylhydroquinone 10′(Z)-heptadecenylhydroquinone [HQ17(1)], isolated from the sap of lacquer tree Rhus succedanea, was found to inhibit activity tyrosinase and suppress melanin production in animal cells. IC50 HQ17(1) as a inhibitor 37 μM versus 70 for hydroquinone (HQ), known melanogenesis. For inhibition mouse B16 melanoma cells, EC50 40 124 HQ. induced much less oxidative stress than did effectiveness inhibiting could be mimicked by intermittent exposure cells HQ17(1). potent...
Defective or imbalanced expression of spliceosomal factors has been linked to human disease; however, how a defective spliceosome affects intron-containing gene transcripts in cells is largely unknown. DEAH-box protein DHX16 orthologue Saccharomyces cerevisiae Prp2, an RNA-dependent ATPase that activates the before first catalytic step splicing. Yeast prp2 mutants accumulate unspliced RNAs from vast majority genes. Here we used genomic tiling microarray screen four chromosomes expressing...
Insulin-like growth factor II (IGF-II) is expressed in many developing embryonic tissues and involved mammalian development. After birth, serum IGF-II mainly produced by liver cells. Many reports have indicated that overexpressed some hepatocellular carcinoma (HCC) tissue. These findings imply the possible importance of this carcinogenesis. We screened four human HCC cell lines three rat found HuH-7 HepG2 cells fivefold more intracellular than other lines. Experimental data indicate...
Human GPKOW [G-patch (glycine-rich) domain and KOW (Kyrpides, Ouzounis Woese) domain] protein contains a G-patch two domains, is homologue of Arabidopsis MOS2 Saccharomyces Spp2 protein. found in the human spliceosome, but its role pre-mRNA splicing remains to be elucidated. In this report, we showed that interacted directly with DHX16/hPRP2 RNA. Immuno-depletion from HeLa nuclear extracts resulted an inactive spliceosome still bound DHX16. Adding back recombinant restored depleted extract....
Natural antimutagens may prevent cancer and are therefore of great interest to oncologists the public at large. Phytochemicals potent antimutagen candidates. When Ames test was applied examine antimutagenic potency supercritical carbon dioxide (SC-CO2) extracts Terminalia catappa leaves a dose 0.5 mg/plate, toxicity mutagenicity were not detected. The activity SC-CO2 increased with decreases temperature (60, 50, 40 °C) pressure (4000, 3000, 2000 psi) used for extraction. most...
In this study, we demonstrated that 10′(Z), 13′(E)-heptadecadienylhydroquinone (HQ17-2), isolated from the lacquer tree, could decrease swarming motility and hemolysin activity but increase polymyxin B (PB) susceptibilityof Proteus mirabilis which is intrinsically highly-resistant to PB. The increased PB susceptibility induced by HQ17-2 was also observed in clinical isolates biofilm-grown cells. inhibit wild-type rppA mutant not rcsB mutant, indicating inhibits through RcsB-dependent...
Two new antioxidative and cytotoxic compounds, 10'(Z),13'(E),15'(E)-heptadecatrienylhydroquinone (1) 10'(Z),13'(E)-heptadecadienylhydroquinone (2), as well the known 10'(Z)-heptadecenylhydroquinone (3), were isolated from an EtOH extract of sap Rhus succedanea. The structures elucidated by spectral analyses. These compounds showed activities against five cancer cell lines.
The formation of a DNA "paper-clip" type triple helix (triplex) with common sequence 5'-d-(TC)3Ta(CT)3Cb(AG)3 (a and b = 0−4) was studied by UV thermal melting experiments CD spectra. These oligomers form triplexes duplexes under slightly acidic neutral conditions, respectively. stability the formed (at pH 4.5) or 7.0 8.0) does not vary significantly size loops 1−4). At 6.0, triplex is, however, function b. It is also interesting to note that oligomer 5'-d-(TC)3(CT)3(AG)3 0) forms stable at...
Abstract Proteomics is a powerful tool for the identification of proteins, which provides basis rational vaccine design. However, it still highly technical and time‐consuming task to examine protein's immunogenicity utilizing traditional approaches. Here, we present platform effectively evaluating protein antibody detection. A tetanus toxin C fragment (Tet‐c) was used as representative antigen establish this platform. cell wall‐anchoring sialidase‐like (SLP) Propionibacterium acnes utilized...
We previously reported that the anticancer activity of a botanical compound 10′(Z),13′(E),15′(E)-heptadecatrienylhydroquinone [HQ17(3)] was attributed to topoisomerase (Topo) IIα poisoning and induction oxidative damage. HQ17(3) irreversibly inhibits Topo in vitro is more cytotoxic leukemia HL-60 cells than IIα-deficient variant HL-60/MX2 cells, which suggests cellular target HQ17(3). This study further characterizes molecular mechanisms Proteomic analyses indicated reacted with Cys-427,...