A5078137593- RNA Research and Splicing
- RNA modifications and cancer
- Problem and Project Based Learning
- RNA and protein synthesis mechanisms
- Education and Critical Thinking Development
- Engineering Education and Curriculum Development
- Lanthanide and Transition Metal Complexes
- Electron Spin Resonance Studies
- Mesenchymal stem cell research
- Immune cells in cancer
- DNA Repair Mechanisms
- Hematopoietic Stem Cell Transplantation
- Radiopharmaceutical Chemistry and Applications
- Cancer, Hypoxia, and Metabolism
- RNA regulation and disease
- Magnetism in coordination complexes
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- DNA and Nucleic Acid Chemistry
- Animal Genetics and Reproduction
- Oral health in cancer treatment
- Analytical Chemistry and Sensors
- Chemokine receptors and signaling
West Virginia University
2012-2023
Morgantown High School
2021
City of Hope
2010-2014
Beckman Research Institute
2010-2014
City Of Hope National Medical Center
2010-2013
West Virginia University Hospitals
2012
Institute of Immunology
2011
University of Edinburgh
2006
Friedrich-Alexander-Universität Erlangen-Nürnberg
2002-2004
Bulgarian Academy of Sciences
1995-1996
ABSTRACT Extracellular vesicles (EVs) are mRNA‐containing cell fragments shed into circulation during pathophysiological events. DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B) regulate gene expression by modifying methylation altering transcription. Sepsis is a systemic insult resulting in vascular dysfunction, which can lead to shock death. We analysed plasma from ICU patients for circulating EV numbers, defined as particles isolated 1 mL at 21,000x g , DNMTs mRNA content prognostic...
Abstract Background Traditionally, doctoral student education in the biomedical sciences relies on didactic coursework to build a foundation of scientific knowledge and an apprenticeship model training laboratory established investigator. Recent recommendations for revision graduate include utilization competencies assess progress introduction novel curricula focused development skills, rather than accumulation facts. Evidence demonstrates that active learning approaches are effective....
There are currently no effective biomarkers for prognosis and optimal treatment selection to improve non-small cell lung cancer (NSCLC) survival outcomes. This study further validated a seven-gene panel diagnosis of NSCLC using RNA sequencing proteomic profiles patient tumors. Within the panel, ZNF71 expression combined with dendritic activities defined subgroups (n = 966) distinct outcomes (p 0.04, Kaplan-Meier analysis). was significantly associated natural killer cells 0.014) T 0.003) in...
Studies of mammalian splicing factors are often focused on small nuclear ribonucleoproteins or regulatory RNA-binding proteins, such as hnRNP (heterogeneous ribonucleoprotein) and SR proteins (serine/arginine-rich proteins); however, much less is known about the contribution DExD/H-box RNA helicases in pre-mRNA splicing. The human DEAH-box protein DHX16 [also DBP2 (DEAD-box 2)], homologous with Caenorhabditis elegans Mog-4, Schizosaccharomyces pombe Prp8 Saccharomyces cerevisiae Prp2. In...
Defective or imbalanced expression of spliceosomal factors has been linked to human disease; however, how a defective spliceosome affects intron-containing gene transcripts in cells is largely unknown. DEAH-box protein DHX16 orthologue Saccharomyces cerevisiae Prp2, an RNA-dependent ATPase that activates the before first catalytic step splicing. Yeast prp2 mutants accumulate unspliced RNAs from vast majority genes. Here we used genomic tiling microarray screen four chromosomes expressing...
Stable tetrathiatriarylmethyl radicals have significantly contributed to the recent progress in biomedical electron paramagnetic resonance (EPR) due their unmatched stability biological media and long relaxation times. However, lipophilic core of most commonly used structure (Finland trityl) is responsible for its interaction with plasma biomacromolecules, such as albumin, self-aggregation at high concentrations and/or low pH. While Finland trityl generally considered inert toward many...
To study the organization of DNA replication in mammalian rRNA genes, sites initiation synthesis rat and human genes were mapped by two independent techniques. In cells growth nascent chains was blocked Trioxsalen cross-links introduced vivo. The fraction “restricted” labeled vivo isolated, abundance this cloned ribosomal sequences determined hybridization. experiments with cells, allowed to grow unrestricted for a certain period time movement forks along followed hybridization...
Human GPKOW [G-patch (glycine-rich) domain and KOW (Kyrpides, Ouzounis Woese) domain] protein contains a G-patch two domains, is homologue of Arabidopsis MOS2 Saccharomyces Spp2 protein. found in the human spliceosome, but its role pre-mRNA splicing remains to be elucidated. In this report, we showed that interacted directly with DHX16/hPRP2 RNA. Immuno-depletion from HeLa nuclear extracts resulted an inactive spliceosome still bound DHX16. Adding back recombinant restored depleted extract....
Our previous study found that zinc finger protein 71 (ZNF71) mRNA expression was associated with chemosensitivity and its prognostic of non-small-cell lung cancer (NSCLC). The Krüppel box (KRAB) transcriptional repression domain is commonly present in human proteins, which are linked to imprinting, silencing repetitive elements, proliferation, apoptosis, cancer. This revealed ZNF71 KRAB had a significantly higher than the KRAB-less isoform NSCLC tumors (n = 197) cell lines 117). Patients...
Mesenchymal stem cells (MSCs) are of interest for use in diverse cellular therapies. Ex vivo expansion MSCs intended transplantation must result generation that maintain fidelity critical functions. Previous investigations have identified genetic and phenotypic alterations with vitro passage, but little is known regarding how culturing influences the ability to repair double strand DNA breaks (DSBs), most severe lesions. To investigate response DSB stress passage , primary human were exposed...
Abstract Background Carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1) is a transmembrane protein with multiple functions in different types. CEACAM1 expression frequently mis-regulated cancer, down-regulation reported several tumors of epithelial origin and de novo lung cancer malignant melanoma. In this report we analyzed the regulation three breast lines that varied from none (MCF7) to moderate (MDA-MB-468) high (MCF10A, comparable normal breast). Results Using vivo footprinting...
Osteoblasts are a major component of the bone marrow microenvironment, which provide support for hematopoietic cell development. Functional disruption any element niche, including osteoblasts, can potentially impair hematopoiesis. We have studied effect two widely used drugs with different mechanisms action, etoposide (VP16) and melphalan, on murine osteoblasts at distinct stages maturation. VP16 melphalan delayed maturation preosteoblasts altered CXCL12 protein levels, key regulator homing...
Hematopoietic reconstitution, following bone marrow or stem cell transplantation, requires a microenvironment niche capable of supporting both immature progenitors and cells with the capacity to differentiate expand. Osteoblasts comprise one important component this niche. We determined that treatment human primary osteoblasts (HOB) melphalan VP-16 resulted in increased phospho-Smad2, consistent TGF-β1 activity. This increase was coincident reduced HOB support B lineage chemotaxis adherence....
Tie2-expressing monocytes/macrophages (TEMs) are a distinct subset of proangiogenic monocytes selectively recruited to tumors in breast cancer. Because the hypoxic nature solid tumors, we investigated if oxygen, via hypoxia-inducible transcription factors HIF-1α and HIF-2α, regulates TEM function tumor microenvironment. We orthotopically implanted PyMT cells into mammary fat pads syngeneic LysMcre,
Hypoxia, acidosis, and elevated inorganic phosphate concentration are characteristics of the tumor microenvironment in solid tumors. There a number methods for measuring each parameter individually vivo, but only method to date noninvasive measurement all three variables simultaneously vivo is electron paramagnetic spectroscopy paired with monophosphonated trityl radical, pTAM/HOPE. While HOPE has been successfully used studies upon intratissue injection, it cannot be delivered intravenously...
We report a new strategy for the synthesis of mono-phosphonated triarylmethyl radical spin probe and standalone application with user-friendly interface automatic spectrum fitting extraction <italic>p</italic>O<sub>2</sub>, pH, [P<sub>i</sub>] values.
Abstract Background Traditionally, doctoral student education in the biomedical sciences relies on didactic coursework to build a foundation of scientific knowledge and an apprenticeship model training laboratory established investigator. Recent recommendations for revision graduate include utilization competencies assess progress introduction novel curricula focused development skills, rather than accumulation facts. Evidence demonstrates that active learning approaches are effective....