A5032547181- Electron Spin Resonance Studies
- Lanthanide and Transition Metal Complexes
- Magnetism in coordination complexes
- Advanced MRI Techniques and Applications
- Advanced NMR Techniques and Applications
- Analytical Chemistry and Sensors
- Cancer, Hypoxia, and Metabolism
- Nitric Oxide and Endothelin Effects
- Organometallic Complex Synthesis and Catalysis
- Microfluidic and Capillary Electrophoresis Applications
- Electrochemical Analysis and Applications
- Nanoparticle-Based Drug Delivery
- Solid-state spectroscopy and crystallography
- Immune Cell Function and Interaction
- Polyoxometalates: Synthesis and Applications
- Adenosine and Purinergic Signaling
- RNA Interference and Gene Delivery
- Neuroendocrine Tumor Research Advances
- Characterization and Applications of Magnetic Nanoparticles
- Synthesis and characterization of novel inorganic/organometallic compounds
- Photoreceptor and optogenetics research
- Innovative Teaching Methods
- Mass Spectrometry Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Protein Interaction Studies and Fluorescence Analysis
West Virginia University
2016-2025
Resonance Research (United States)
2023
Morgantown High School
2019-2021
Health Sciences Centre
2020
UCLouvain
2006-2016
Noninvasive in vivo assessment of chemical tumor microenvironment (TME) parameters such as oxygen (pO2), extracellular acidosis (pHe), and concentration interstitial inorganic phosphate (Pi) may provide unique insights into biological processes solid tumors. In this work, we employ a recently developed multifunctional trityl paramagnetic probe electron resonance (EPR) technique for concurrent these TME various mouse models cancer. While the data support existence hypoxic acidic regions TME,...
A stable triarylmethyl spin probe whose electron paramagnetic resonance (EPR) spectrum is highly sensitive to molecular tumbling reported. The strong anisotropy of the hyperfine coupling tensor with central carbon a
We report the synthesis of hydroxyethyl tetrathiatriarylmethyl radical OX063 and its deuterated analogue OX071 for biomedical EPR applications.
Sensitive in-cell distance measurements in proteins using pulsed-electron spin resonance (ESR) require reduction-resistant and cleavage-resistant labels. Among the moieties, hydrophilic trityl core known as OX063 is promising due to its long phase-memory relaxation time (Tm). This property leads a sufficiently intense ESR signal for reliable measurements. Furthermore, Tm of remains at higher temperatures, opening possibility temperatures above 50 K. In this work, we synthesized deuterated...
Abstract Background We investigated the role of A2B-adenosine receptor in regulating immunosuppressive metabolic stress tumor microenvironment. Novel antagonist PBF-1129 was tested for antitumor activity mice and evaluated safety immunologic efficacy a phase I clinical trial patients with non-small cell lung cancer. Methods The antagonists their impact on immune microenvironment were lung, melanoma, colon, breast, epidermal growth factor receptor–inducible transgenic cancer models. Employing...
In vivo measurement and mapping of oxygen levels within the tissues are crucial in understanding physiopathological processes numerous diseases, such as cancer, diabetes, or peripheral vascular diseases. Electron paramagnetic resonance (EPR) associated with biocompatible exogenous spin probes, Ox071 triarylmethyl (TAM) radical, is becoming new gold standard for preclinical settings. However, these probes do not show tissue selectivity when injected systemically, they cell permeable,...
Abstract Electron paramagnetic resonance (EPR) is an accurate and efficient technique to probe unpaired electrons in many applications across materials science, chemistry, biology. Dynamic processes are investigated using EPR; however, these limited by the use of resonator-based spectrometers such that entire process must be confined resonator. The EPR-on-a-chip (EPRoC) device circumvents this limitation integrating EPR spectrometer into a single microchip. In approach, coil...
A new water soluble phosphonated tetrathiatriarylmethyl radical has been synthesized and its application for pH measurement in a physiological range by EPR is reported.
We report that tetrathiatriarylmethyl (trityl) EPR probes are chiral molecules at room temperature, the two stereoisomers differ in their helicity being configurationally stable enough to be separated and stored independently.
As superoxide anion is of keen interest in biomedical research, it highly desirable to have a technique allowing its detection sensitively and specifically biological media. If electron paramagnetic resonance (EPR) techniques probes been individually described the literature, there actually no comparison these same conditions that may help guiding researchers for selecting most appropriate approach. The aim present study was compare different EPR strategies terms sensitivity specificity...
Purpose The ability to assess the extracellular pH (pHe) is an important issue in oncology, because acidification associated with tumor aggressiveness and resistance cytotoxic therapies. In this study, a stable triphosphonated triarylmethyl (TPTAM) radical was qualified as pHe electron paramagnetic resonance (EPR) molecular reporter. Methods Calibration of hyperfine splitting function performed using 1.2-GHz EPR spectrometer. Gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA) used...
Stable tetrathiatriarylmethyl radicals have significantly contributed to the recent progress in biomedical electron paramagnetic resonance (EPR) due their unmatched stability biological media and long relaxation times. However, lipophilic core of most commonly used structure (Finland trityl) is responsible for its interaction with plasma biomacromolecules, such as albumin, self-aggregation at high concentrations and/or low pH. While Finland trityl generally considered inert toward many...
Abstract GM-CSF has been employed as an adjuvant to cancer immunotherapy with mixed results based on dosage. We previously showed that regulated tumor angiogenesis by stimulating soluble vascular endothelial growth factor (VEGF) receptor-1 from monocytes/macrophages in a dose-dependent manner neutralized free VEGF, and intratumoral injections of high-dose ablated blood vessels worsened hypoxia orthotopic polyoma middle T Ag (PyMT) triple-negative breast (TNBC). In this study, we assessed...
Superoxide radical represents one of the most biologically relevant reactive oxygen species involved in numerous physiological and pathophysiological processes. measurement through decay an electron paramagnetic resonance (EPR) signal a triarylmethyl (TAM) possesses advantage high selectivity relatively rate constant TAM reaction with superoxide. Hereby we report straightforward synthesis characterization TAM-TAM biradical showing reactivity superoxide (second-order constant, (6.7 ± 0.2) × 10
This protocol demonstrates the capability of low-field electron paramagnetic resonance (EPR)-based techniques in combination with functional probes to provide quantitative information on chemical tumor microenvironment (TME), including pO2, pH, redox status, concentrations interstitial inorganic phosphate (Pi), and intracellular glutathione (GSH). In particular, an application a recently developed soluble multifunctional trityl probe provides unsurpassed opportunity for vivo concurrent...
We describe the synthesis, characterization, and application of an isotopologue trityl radical OX071, labeled with 13C at central carbon (13C1). This spin probe features large anisotropy hyperfine coupling 13C1 (I = 1/2), leading to EPR spectrum highly sensitive molecular tumbling. The high biocompatibility lack interaction blood albumin allow for systemic delivery in vivo measurement tissue microviscosity by EPR.
Alterations in viscosity of biological fluids and tissues play an important role health diseases. It has been demonstrated that the electron paramagnetic resonance (EPR) spectrum a 13C-labeled trityl spin probe (13C-dFT) is highly sensitive to local its microenvironment. In present study, we demonstrate X-band (9.5 GHz) EPR viscometry using 13C-dFT provides simple tool accurately measure microviscosity human blood microliter volumes obtained from healthy volunteers. An application low-field...
Dynamic nuclear polarization (DNP) is an NMR sensitivity enhancement technique that mediates transfer from unpaired electrons to NMR-active nuclei. Despite its success in elucidating important structural information on biological and inorganic materials, the detailed polarization-transfer pathway nearby then bulk solvent nuclei, finally molecules of interest-remains unclear. In particular, nuclei paramagnetic polarizing agent play significant roles relaying enhanced polarizations more remote...