A5029282918- Electron Spin Resonance Studies
- Lanthanide and Transition Metal Complexes
- Advanced MRI Techniques and Applications
- Magnetism in coordination complexes
- Advanced NMR Techniques and Applications
- Analytical Chemistry and Sensors
- Cellular and Composite Structures
- Elasticity and Material Modeling
- Nonlocal and gradient elasticity in micro/nano structures
- Photoreceptor and optogenetics research
- Microbial Inactivation Methods
- Synthesis of Indole Derivatives
- Microfluidic and Capillary Electrophoresis Applications
West Virginia University
2017-2025
Morgantown High School
2019-2021
In vivo measurement and mapping of oxygen levels within the tissues are crucial in understanding physiopathological processes numerous diseases, such as cancer, diabetes, or peripheral vascular diseases. Electron paramagnetic resonance (EPR) associated with biocompatible exogenous spin probes, Ox071 triarylmethyl (TAM) radical, is becoming new gold standard for preclinical settings. However, these probes do not show tissue selectivity when injected systemically, they cell permeable,...
A stable triarylmethyl spin probe whose electron paramagnetic resonance (EPR) spectrum is highly sensitive to molecular tumbling reported. The strong anisotropy of the hyperfine coupling tensor with central carbon a
We report the synthesis of hydroxyethyl tetrathiatriarylmethyl radical OX063 and its deuterated analogue OX071 for biomedical EPR applications.
Abstract Electron paramagnetic resonance (EPR) is an accurate and efficient technique to probe unpaired electrons in many applications across materials science, chemistry, biology. Dynamic processes are investigated using EPR; however, these limited by the use of resonator-based spectrometers such that entire process must be confined resonator. The EPR-on-a-chip (EPRoC) device circumvents this limitation integrating EPR spectrometer into a single microchip. In approach, coil...
Stable tetrathiatriarylmethyl radicals have significantly contributed to the recent progress in biomedical electron paramagnetic resonance (EPR) due their unmatched stability biological media and long relaxation times. However, lipophilic core of most commonly used structure (Finland trityl) is responsible for its interaction with plasma biomacromolecules, such as albumin, self-aggregation at high concentrations and/or low pH. While Finland trityl generally considered inert toward many...
Superoxide radical represents one of the most biologically relevant reactive oxygen species involved in numerous physiological and pathophysiological processes. measurement through decay an electron paramagnetic resonance (EPR) signal a triarylmethyl (TAM) possesses advantage high selectivity relatively rate constant TAM reaction with superoxide. Hereby we report straightforward synthesis characterization TAM-TAM biradical showing reactivity superoxide (second-order constant, (6.7 ± 0.2) × 10
We describe the synthesis, characterization, and application of an isotopologue trityl radical OX071, labeled with 13C at central carbon (13C1). This spin probe features large anisotropy hyperfine coupling 13C1 (I = 1/2), leading to EPR spectrum highly sensitive molecular tumbling. The high biocompatibility lack interaction blood albumin allow for systemic delivery in vivo measurement tissue microviscosity by EPR.
Alterations in viscosity of biological fluids and tissues play an important role health diseases. It has been demonstrated that the electron paramagnetic resonance (EPR) spectrum a 13C-labeled trityl spin probe (13C-dFT) is highly sensitive to local its microenvironment. In present study, we demonstrate X-band (9.5 GHz) EPR viscometry using 13C-dFT provides simple tool accurately measure microviscosity human blood microliter volumes obtained from healthy volunteers. An application low-field...
Enzyme catalytic activities are critical biomarkers of tissue states under physiological and pathophysiological conditions. However, the direct measurement imaging enzyme activity in vivo remains extremely challenging. We report synthesis characterization first stable triarylmethyl (TAM) radical substrate alkaline phosphatase (TAM-ALPs). The enzymatic dephosphorylation TAM-ALPs results a drastic change its electron paramagnetic resonance (EPR) spectrum that can be used to image using...
Abstract Tumor oxygenation ( p O 2 ), acidosis (pH) and interstitial inorganic phosphate concentration (Pi) are important parameters of the malignant behavior cancer. A noninvasive procedure that enables visualization these may provide unique information about mechanisms tumor pathophysiology clues to new treatment targets. In this research, we present a multiparametric imaging method allowing for concurrent mapping pH, spin probe concentration, , Pi using single contrast agent...
Hypoxia, acidosis, and elevated inorganic phosphate concentration are characteristics of the tumor microenvironment in solid tumors. There a number methods for measuring each parameter individually vivo, but only method to date noninvasive measurement all three variables simultaneously vivo is electron paramagnetic spectroscopy paired with monophosphonated trityl radical, pTAM/HOPE. While HOPE has been successfully used studies upon intratissue injection, it cannot be delivered intravenously...
Soluble stable radicals are used as spin probes and labels for in vitro vivo electron paramagnetic resonance (EPR) spectroscopy imaging applications. We report the synthesis characterization of a perchlorinated triarylmethyl radical enriched 99% at central carbon, 13C1–PTMTC. The anisotropy hyperfine splitting with 13C1 (Ax = 26, Ay 25, Az 199.5 MHz) g (gx 2.0015, gy gz 2.0040) responsible strong effect tumbling rate on EPR spectrum. rotational correlation time can be determined by spectral...
We report a new strategy for the synthesis of mono-phosphonated triarylmethyl radical spin probe and standalone application with user-friendly interface automatic spectrum fitting extraction <italic>p</italic>O<sub>2</sub>, pH, [P<sub>i</sub>] values.
Abstract A stable triarylmethyl spin probe whose electron paramagnetic resonance (EPR) spectrum is highly sensitive to molecular tumbling reported. The strong anisotropy of the hyperfine coupling tensor with central carbon a 13 C 1 ‐labeled radical enables measurement rotational correlation time applications measure microviscosity and dynamics.