A5073174768- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Spectroscopy Techniques in Biomedical and Chemical Research
- Optical Coherence Tomography Applications
- Cell Image Analysis Techniques
- Near-Field Optical Microscopy
- Spectroscopy and Chemometric Analyses
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Ultrasound and Hyperthermia Applications
- Molecular Biology Techniques and Applications
- Nanoplatforms for cancer theranostics
- Ultrasound and Cavitation Phenomena
- Image Processing Techniques and Applications
- Neural dynamics and brain function
- Thermography and Photoacoustic Techniques
- Optical Imaging and Spectroscopy Techniques
- Ultrasound Imaging and Elastography
- Quantum Dots Synthesis And Properties
- Advanced Electron Microscopy Techniques and Applications
- Phase-change materials and chalcogenides
- Photonic and Optical Devices
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Biosensing Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
Imperial College London
2013-2025
London Centre for Nanotechnology
2020-2022
Massachusetts Institute of Technology
2013-2019
University of Cambridge
2008-2018
IIT@MIT
2016
Bellingham Technical College
2015
University of Nottingham
2011-2014
Significance Histopathology is the standard method for diagnosis of cancer. However, this requires time-consuming procedures sectioning and staining tissues, making histopathology impractical use during surgery most cancer types. We report a unique based on two optical spectroscopy techniques—autofluorescence imaging Raman scattering—that can accurately measure molecular differences between tumor cells healthy tissue allows tumors faster than histopathology, without requiring or staining....
Motor units are the fundamental elements responsible for muscle movement. They formed by lower motor neurons and their targets, synapsed via neuromuscular junctions (NMJs). The loss of NMJs in neurodegenerative disorders (such as amyotrophic lateral sclerosis or spinal atrophy) a result traumatic injuries affects millions lives each year. Developing vitro assays that closely recapitulate physiology tissues is crucial to understand formation maturation NMJs, well help unravel mechanisms...
This review summarizes the historical and more recent developments of multiphoton microscopy, as applied to dermatology. Multiphoton microscopy offers several advantages over competing techniques: there is an inherent axial sectioning, penetration depths that compete well with confocal on account use near-infrared light, many two-photon contrast mechanisms, such second-harmonic generation, have no analogue in one-photon microscopy. While photons into tissue are typically limited order...
Significance Malaria parasites degrade substantial quantities of hemoglobin to release heme within a specialized digestive vacuole. Most this is sequestered in an inert crystal. However, the concentration bioavailable, labile parasite’s cytosol was unknown. We developed biosensor provide first quantitative insights into concentrations malaria parasites. find that ∼1.6 µM cytosolic maintained, including during period coincident with intense degradation. The heme-binding antimalarial drug,...
Abstract Three-photon wide-field depth-resolved excitation is used to overcome some of the limitations in conventional point-scanning two- and three-photon microscopy. Excitation chromophores as diverse channelrhodopsins quantum dots shown, a penetration depth more than 700 μm into fixed scattering brain tissue achieved, approximately twice deep that achieved using two-photon excitation. Compatibility with live animal experiments confirmed by imaging cerebral vasculature an anesthetized...
A method is presented for acquiring high-spatial-resolution spectral maps, in particular Raman micro-spectroscopy (RMS), by selectively sampling the spatial features of interest and interpolating results. This achieves up to 30 times reduction time compared raster-scanning, resulting images have excellent correlation with conventional histopathological staining, are achieved sufficient signal-to-noise ratio identify individual tissue structures. The benefits this selective not limited...
Super-resolution ultrasound (SRUS) imaging through localising and tracking sparse microbubbles has been shown to reveal microvascular structure flow beyond the wave diffraction limit. Most SRUS studies use standard delay sum (DAS) beamforming, where large main lobe significant side lobes make separation localisation of densely distributed bubbles challenging, particularly in 3D due typically small aperture matrix array probes. This study aims improve by implementing a low-cost coherence...
Focused ultrasound stimulation of microbubbles is being tested in clinical trials for its ability to deliver drugs across the blood-brain barrier (BBB). This technique has potential treat neurological diseases by preferentially delivering targeted regions. Yet despite potential, physical mechanisms which alter BBB permeability remain unclear, as direct observations oscillating brain microvessels have never been previously recorded. The purpose this study was reveal how respond when within...
Super-resolution single-molecule localization microscopy, often referred to as PALM/STORM, works by ensuring that fewer than one fluorophore in a diffraction-limited volume is emitting at any time, allowing the observer infer emitter located center of point-spread function. This requires careful control over incident light intensity order rate which fluorophores are switched on; if too many activated, their functions overlap, impedes efficient localization. If few imaging time impractically...
Abstract Structured Illumination Microscopy, SIM, is one of the most powerful optical imaging methods available to visualize biological environments at subcellular resolution. Its limitations stem from a difficulty in multiple color channels once, which reduces speed. Furthermore, there substantial experimental complexity setting up SIM systems, preventing widespread adoption. Here, we present Machine-learning Assisted, Interferometric MAI-SIM, as an easy-to-implement method for live cell...
Abstract A method for the automatic removal of a baseline in various types spectra is proposed. The local values second derivative are used to distinguish between peaks and background, algorithm requires no user input. performance arguably better than all others tested low‐noise environments with varying intensities, comparable best routines case low signal‐to‐noise ratio. Copyright © 2010 John Wiley & Sons, Ltd.
Microfluidics has enabled low volume biochemistry reactions to be carried out at the point-of-care. A key component in microfluidics is microfluidic valve. Microfluidic valves are not only useful for directing flow intersections but also allow mixtures/dilutions tuned real-time and even provide peristaltic pumping capabilities. In transition from chip-in-a-lab lab-on-a-chip, it essential ensure that designed require less peripheral equipment they transportable. this paper, a...
Biosynthesis in bioreactors plays a vital role many applications, but tools for accurate situ monitoring of the cells are still lacking. By engineering such that their conditions reported through fluorescence, it is possible to fill gap using fluorescence diffuse optical tomography (fDOT). However, spatial accuracy reconstruction can be limited, due e.g. undersampling and inaccurate estimation properties. Utilizing controlled phantom studies, we use two-step hybrid approach, where...
Mechanical effects of microbubbles on tissues are central to many emerging ultrasound applications. Here, we investigated the acoustic radiation force a microbubble exerts tissue at clinically relevant therapeutic parameters. Individual administered into wall-less hydrogel channel (diameter: 25-100 µm, Young's modulus: 2-8.7 kPa) were exposed an pulse (centre frequency: 1 MHz, length: 10 ms, peak-rarefactional pressures: 0.6-1.0 MPa). Using high-speed microscopy, each was tracked as it...
A selective scanning method was used to measure spatially resolved Raman spectra of live Neospora caninum tachyzoites colonizing human brain microvascular-endothelial cells. The technique allowed the detection nucleic acids, lipids and proteins linked parasites their cellular micro-environment at ∼10× shorter acquisition time compared raster scanning.
Simultaneous, high-resolution imaging across a large number of synaptic and dendritic sites is critical for understanding how neurons receive integrate signals. Yet, functional that targets submicrometer-sized locations poses significant technical challenges. We demonstrate new parallelized approach to address such questions, increasing the signal-to-noise ratio by an order magnitude compared previous approaches. This selective access multifocal multiphoton microscopy uses spatial light...
We describe chalcogenide glass (ChG)-based nanostructures for use as substrates surface-enhanced Raman scattering (SERS). Such were fabricated by exploiting the photosensitivity of ChG. This allows convenient control shape, size, and spacing nanostructures. The used to investigate sample-concentration excitation-power dependences SERS from Rhodamine 6G molecules. A sensitivity 1 muM was achieved at low excitation irradiance, a semilinear concentration dependence found concentrations below...
ABSTRACT Background Focused ultrasound stimulation of microbubbles is being tested in clinical trials for its ability to deliver drugs across the blood-brain barrier (BBB). This technique has potential treat neurological diseases by preferentially delivering targeted regions. Yet despite potential, physical mechanisms which alter BBB permeability remain unclear, as direct observations oscillating cerebral capillaries have never been previously recorded. The purpose this study was reveal how...
Temporal focusing multiphoton microscopy is a technique for performing highly parallelized while still maintaining depth discrimination. While the conventional wide-field configuration temporal suffers from sub-optimal axial resolution, line scanning focusing, implemented here using digital micromirror device (DMD), can provide substantial improvement. The DMD-based dynamically trades off degree of parallelization, and hence imaging speed, allowing performance parameters to be adapted...
Spontaneous Raman imaging has emerged as powerful label-free technique for investigating the molecular composition of medicines and biological specimens. Although can facilitate understanding complex phenomena in vivo, current modalities are limited speed sample compatibility. Here, we introduce a single-objective line-scanning light-sheet microscope, named <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline" overflow="scroll"> <mml:mi>λ</mml:mi> </mml:math> -OPM, which...