A5091751015- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- MicroRNA in disease regulation
- Memory and Neural Mechanisms
- Neurological Disease Mechanisms and Treatments
- Immune cells in cancer
- Ion channel regulation and function
- Zebrafish Biomedical Research Applications
- Atherosclerosis and Cardiovascular Diseases
- Heat shock proteins research
- Digestive system and related health
- Systemic Lupus Erythematosus Research
- Telecommunications and Broadcasting Technologies
- Nerve injury and regeneration
- Developmental Biology and Gene Regulation
- Aquaculture disease management and microbiota
- Adenosine and Purinergic Signaling
- Lysosomal Storage Disorders Research
- Liver physiology and pathology
- Marine Invertebrate Physiology and Ecology
- Inflammation biomarkers and pathways
- Circadian rhythm and melatonin
- Millimeter-Wave Propagation and Modeling
- Neurological disorders and treatments
YKK (Japan)
2025
University College London
2016-2024
Wolfson Foundation
2017-2024
Nagasaki University
2021-2023
Vanderbilt University
2019
Kyoto University
2019
The Graduate University for Advanced Studies, SOKENDAI
2009-2018
National Institute for Physiological Sciences
2009-2018
Kōchi University
2014-2018
Shimizu (Japan)
2017
Myelin, made by oligodendrocytes, is essential for rapid information transfer in the central nervous system. Oligodendrocyte precursors (OPs) receive glutamatergic synaptic input from axons but how this affects their development unclear. Murine OPs white matter express AMPA receptor (AMPAR) subunits GluA2, GluA3 and GluA4. We generated mice which lack both GluA2 GluA3, or all three GluA2/3/4, respectively reduced abolished AMPAR-mediated to OPs. In double- triple-knockouts OP proliferation...
Abstract Background Microglia-derived lysosomal cathepsins are important inflammatory mediators to trigger signaling pathways in inflammation-related cascades. Our previous study showed that the expression of cathepsin C (CatC) brain is induced predominantly activated microglia neuroinflammation. Moreover, CatC can induce chemokine production processes. In vitro studies further confirmed secreted extracellularly from LPS-treated microglia. However, mechanisms affecting neuroinflammatory...
Abstract Previous work has shown that motor skill learning stimulates and requires generation of myelinating oligodendrocytes (OLs) from their precursor cells (OLPs) in the brains adult mice. In present study we ask whether OL production is also required for non-motor cognition, using T-maze radial-arm-maze tasks tax spatial working memory. We find maze training OLP proliferation medial prefrontal cortex (mPFC), anterior corpus callosum (genu), dorsal thalamus hippocampal formation male...
The mediobasal hypothalamus (MBH; arcuate nucleus of the [ARH] and median eminence [ME]) is a key nutrient sensing site for production complex homeostatic feedback responses required maintenance energy balance. Here, we show that refeeding after an overnight fast rapidly triggers proliferation differentiation oligodendrocyte progenitors, leading to new oligodendrocytes in ME specifically. During this nutritional paradigm, perineuronal nets (PNNs), emerging regulators ARH metabolic functions,...
Abstract Demyelination coincides with numerous changes of gene expression in the central nervous system (CNS). Cystatin F, which is a papain‐like lysosomal cysteine proteinase inhibitor that normally expressed by immune cells and not brain, massively induced CNS during acute demyelination. We found microglia, are monocyte/macrophage‐lineage CNS, express cystatin F only By using several demyelinating animal models spinal cord tissues from multiple sclerosis (MS) patients, we examined...
The aim of this study was to investigate the role peroxiredoxin 1 (Prdx1) in invasiveness pancreatic ductal adenocarcinoma (PDAC) cells.Immunohistochemistry used determine overexpression Prdx1 human PDAC tissues. Immunoprecipitation and immunocytochemistry were interaction intracellular distribution a member mitogen-activated protein kinase (MAPK) family protein, p38 MAPK, cells. Finally, Matrigel invasion assay examine effects MAPK on formation cell protrusions invasion.Prdx1 is...
Oligodendrocyte progenitor cell differentiation is regulated by nutritional signals in the adult median eminence (ME), but consequences on local myelination are unknown. The aim of this study was to characterize myelin plasticity ME mice health or response chronic challenge and determine its relevance regulation energy balance.
Although the precise mechanism underlying initial lesion development in multiple sclerosis (MS) remains unclear, CNS inflammation has long been associated with demyelination and axonal degeneration. The activation of microglia/macrophages, which serve as innate immune cells CNS, is first reaction to even minor pathologic changes considered an pathogenic event MS. Microglial accompanies a variety gene expressions, including cystatin F (Cys F), belongs superfamily one cathepsin inhibitors. In...
HOX genes encode transcription factors that function as sequence-specific are involved in cellular proliferation, differentiation, and death. The aim of this study was to investigate the role a family protein, HOXB7, motility invasiveness pancreatic cancer cells. We previously identified HOXB7 transcript is one number transcripts preferentially translated membrane protrusions Immunocytochemistry showed localized cell migrating Knockdown by transfection with HOXB7-specific siRNA decreased...
In demyelinating diseases such as multiple sclerosis (MS), an imbalance between the demyelination and remyelination rates underlies degenerative processes. Microglial activation is observed in lesions; however, molecular mechanism responsible for homeostatic/environmental change remains elusive. We previously found that cystatin F (CysF), a cysteine protease inhibitor, selectively expressed microglia only actively demyelinating/remyelinating lesions but ceases expression chronic lesions,...
Gastric chief cells differentiate from mucous neck and develop their mature state at the base of oxyntic glands with expression secretory zymogen granules. After parietal cell loss, transdifferentiate into metaplasia, designated spasmolytic polypeptide-expressing metaplasia (SPEM), which is considered a candidate precursor gastric cancer. We examined range microRNA (miRNA) in identified miRNAs involved transdifferentiation SPEM. Among them, miR-148a was strongly specifically expressed...
Abstract Oligodendrocytes continue to differentiate from their precursor cells even in adulthood, a process that can be modulated by neuronal activity and experience. Previous work has indicated conditional ablation of oligodendrogenesis adult mice leads learning memory deficits range behavioral tasks. The current study replicated re‐evaluated evidence for role motor learning, using complex running wheel task. Further, we found ablating alters brain microstructure (ex vivo MRI) (in EEG)...
Abstract In chronic demyelinating lesions of the central nervous system, insufficient generation oligodendrocytes (OLs) is not due to a lack oligodendrocyte precursor cells (OPCs), because accumulation OPCs and premyelinating OLs can be observed within these lesions. Here we sought identify basis for failure achieve terminal differentiation in through utilization plp1 ‐overexpressing ( Plp tg /− ) mice. These mice are characterized by progressive demyelination young adults at more mature...
Multiple sclerosis (MS) is an autoimmune disease characterized by immune-mediated inflammation, which attacks the myelin sheath. MS pursues a relapsing and remitting course with varying intervals between symptoms. The main clinical pathological features include sheath destruction plaque formation in central nervous system (CNS). We previously reported that cystatin F (CysF) expression induced demyelinating lesions are accompanied active remyelination (referred to as shadow plaques) but...
Abstract Oligodendrocytes continue to differentiate from their precursor cells even in adulthood, a process that can be modulated by neuronal activity and experience. Yet, our understanding of the functional role adult oligodendrogenesis remains limited. Previous work has indicated conditional ablation mice lead learning memory deficits range behavioural tasks. Our results, reported here, have replicated key finding run on complex wheel with unevenly spaced rungs is disrupted...
Summary Motor skill learning stimulates and requires generation of myelinating oligodendrocytes (OLs) from their precursors (OLPs). We asked whether OL production is also required for non-motor cognition, using T-maze radial arm maze tasks that tax spatial working memory. Maze training stimulated in the medial prefrontal cortex (mPFC), anterior corpus callosum (genu), dorsal thalamus hippocampal formation; myelin sheath formation was genu. Genetic blockade differentiation neo-myelination...
Abstract This methods paper details a protocol to test spatial working memory in mice using semi-automated radial 8-arm maze (RAM). The RAM is partially enclosed apparatus with 8 horizontal equally spaced arms radiating from central hub, which access each arm can be controlled individually by servo-controlled motorized doors. Animals start the hub and are allowed explore for food reward at end of or selected arms. task was originally designed rats but we have adapted – example, including...