A5022953381- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Pain Mechanisms and Treatments
- Neuroscience of respiration and sleep
- Neural dynamics and brain function
- Olfactory and Sensory Function Studies
- Nerve injury and regeneration
- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Ion Channels and Receptors
- Neuroinflammation and Neurodegeneration Mechanisms
- Vestibular and auditory disorders
- Biochemical Analysis and Sensing Techniques
- ATP Synthase and ATPases Research
- Neurogenesis and neuroplasticity mechanisms
- Sleep and Wakefulness Research
- Neurobiology and Insect Physiology Research
- Hearing, Cochlea, Tinnitus, Genetics
- Functional Brain Connectivity Studies
- Ion Transport and Channel Regulation
- Mitochondrial Function and Pathology
- stochastic dynamics and bifurcation
- Advanced Neuroimaging Techniques and Applications
- Electrochemical Analysis and Applications
- Cellular transport and secretion
Nagasaki University
2025
Iwate Medical University
2009-2020
Tokyo Medical University
1988-2018
Okayama University Hospital
2017
Tsurumi University
2009-2012
Tokyo Dental College
2012
National Center of Neurology and Psychiatry
2002-2010
American Association of Endodontists
2010
The University of Tokyo
2001-2002
Tokyo Medical and Dental University
1982-2001
At many central excitatory synapses, AMPA receptors relay the electrical signal, whereas activation of NMDA is conditional and serves a modulatory function. We show here quite different role for at dendrodendritic synapses between mitral granule cells in rat olfactory bulb. In whole-cell patch-clamp recordings bulb slices, stimulation elicited slowly decaying, GABAA receptor-mediated reciprocal IPSCs that reflected prolonged GABA release from cells. Although had normal complement receptors,...
At nicotinic and glutamatergic synapses, the duration of postsynaptic response depends on affinity receptor for transmitter (Colquhoun et al., 1977; Pan 1993). Affinity is often thought to be determined by ligand unbinding rate, whereas binding rate assumed diffusion-limited. In this view, selects those ligands that form a stable complex binding, but uniformly fast does not itself affect selectivity. We tested these assumptions GABA A dissecting contributions microscopic kinetics agonists...
The modulation of high-threshold Ca2+ currents by the selective metabotropic glutamate receptor (mGluR) agonist (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD), was investigated in cultured hippocampal neurons using whole-cell voltage-clamp recording. ACPD reduced carried Ba2+ with an EC50 15.5 microM. inhibition reversible, voltage dependent, and blocked L-2-amino-3-phosphonopropionic (1 mM) or pretreatment pertussis toxin. Inhibition greatly enhanced, became irreversible, when...
Duchenne muscular dystrophy (DMD) is accompanied by cognitive deficits and psychiatric symptoms. In the brain, dystrophin, protein responsible for DMD, localized to a subset of GABAergic synapses, but its role in brain function has not fully been addressed. Here, we report that defensive behaviour, response danger or threat, enhanced dystrophin-deficient mdx mice. Mdx mice consistently showed potent freezing responses brief restraint never induced such wild-type Unconditioned conditioned...
Through tropo-myosine-related kinase B (TrkB) receptors, brain-derived neurotrophic factor (BDNF) performs many biological functions such as neural survival, differentiation, and plasticity. T1, an isoform of TrkB receptors that lacks a tyrosine kinase, predominates in the adult mammalian CNS, yet its role remains controversial. In this study, to examine whether T1 transduces signal determine function, we first performed affinity purification T1-binding protein with T1-specific C-terminal...
To determine the subunit composition of high-affinity kainate receptors in native neurons is a challenging problem because expression more than one GluR subunit. In present study question whether GluR5 and/or GluR6 subunits combine with KA-1 or KA-2 vivo addressed by performing detailed physiological, pharmacological, and molecular characterization functional receptor channels acutely dissociated trigeminal ganglion (TG) neurons. The results show that (1) smaller diameter TG (<30 μm)...
AMPA and NMDA receptor channels are closely related molecules, yet they respond to glutamate with distinct kinetics, attributable differences in ligand binding channel gating steps (for review, seeEdmonds et al., 1995). We used two complementary approaches investigate the number of functional sites on outside-out patches from cultured hippocampal neurons. The activation kinetics agonist were measured during rapid into low concentrations selective agonists a competitive antagonist,...
Individual primary vestibular afferents exhibit spontaneous activity the regularity of which can vary from regular to irregular. Different aspects responsiveness have been associated with this dimension resting discharge. Isolated rat ganglion cells (VGCs) showed heterogeneous intrinsic firing properties during sustained membrane depolarization: some neurons exhibited a strong adaptation generating just single or few spikes (phasic type), whereas other moderate tonic (tonic type). Tonic...
Abstract By altering their morphology, astrocytes, including those involved in the maintenance and plasticity of neurons clearance transmitter, play important roles synaptic transmission; however, mechanism that regulates morphological astrocytes remains unclear. Recently, we reported T1, a subtype TrkB (a family BDNF‐specific receptors), altered astrocytic morphology through control Rho GTPases primary astrocyte cultures. In this study, extended observation to investigate acute neocortical...
Lysophosphatidic acid receptor 1 (LPA1) plays a pivotal role in the pathophysiology of various diseases, especially chronic pain. In this study, we assessed biochemical properties Compound A, novel LPA1 antagonist and its beneficial effects fibromyalgia (FM)-like pain model. A was found to be high-affinity selective have high brain penetrability. Repeated oral administrations reversed hyperalgesia as late 9 days after treatments, suggesting compound has curative effect FM
1 Paired whole-cell recordings were made from a glutamatergic giant nerve terminal, the calyx of Held, and its postsynaptic target cell in medial nucleus trapezoid body (MNTB) brainstem slice juvenile rat. Excitatory currents (EPSCs) evoked by presynaptic action potentials triggered brief (1 ms) depolarizing pulses. 2 In normal artificial cerebrospinal fluid (ACSF), EPSCs several nanoamperes amplitude at relatively constant latency with no failure, whereas low [Ca2+]o-high [Mg2+]o solutions,...
Abstract The effects of α‐pompilidotoxin (α‐PMTX), a new neurotoxin isolated from the venom solitary wasp, were studied on neuromuscular synapses in lobster walking leg and rat trigeminal ganglion (TG) neurons. Paired intracellular recordings presynaptic axon terminals innervating muscles revealed that α‐PMTX induced long bursts action potentials axon, which resulted facilitated excitatory inhibitory synaptic transmission. was distinct other known facilitatory toxins, including sea anemone...
17 beta-Estradiol (E2) has been shown to up-regulate the binding activity of TRH receptor in rat pituitary cells. In this report, we investigated whether and how E2 alters expression at mRNA level using oocyte system, Northern blot analysis, nuclear run-on assay. oocytes injected with from anterior pituitaries or GH3 cells, a TRH-induced 45Ca2+ efflux appeared. This was dependent on amount RNA, inhibited by simultaneous addition chloridazepoxide, an antagonist receptor. Treatment cells...
In demyelinating diseases such as multiple sclerosis (MS), an imbalance between the demyelination and remyelination rates underlies degenerative processes. Microglial activation is observed in lesions; however, molecular mechanism responsible for homeostatic/environmental change remains elusive. We previously found that cystatin F (CysF), a cysteine protease inhibitor, selectively expressed microglia only actively demyelinating/remyelinating lesions but ceases expression chronic lesions,...