A5088441434- Bone Metabolism and Diseases
- Bone health and treatments
- Ion channel regulation and function
- Mesenchymal stem cell research
- Trace Elements in Health
- Cancer Cells and Metastasis
- Obstructive Sleep Apnea Research
- Periodontal Regeneration and Treatments
- NF-κB Signaling Pathways
- Bone and Dental Protein Studies
- Bone Tissue Engineering Materials
- Immune Response and Inflammation
- Oral microbiology and periodontitis research
- Biochemical Analysis and Sensing Techniques
- Chemokine receptors and signaling
- Magnesium in Health and Disease
- Signaling Pathways in Disease
- Laser Applications in Dentistry and Medicine
- Olfactory and Sensory Function Studies
- Wnt/β-catenin signaling in development and cancer
- dental development and anomalies
- Neurobiology and Insect Physiology Research
- Ion Channels and Receptors
- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
Fukuoka Dental College
2016-2025
American Association of Endodontists
2010
Kyushu Dental University
2009
Department of Physiological Sciences
2005
University of Pittsburgh
2004-2005
Notch signaling plays a key role in various cell differentiation processes including bone homeostasis. However, the specific involvement of regulating osteoclastogenesis is still controversial. In present study, we show that RANKL induces expression Jagged1 and Notch2 marrow macrophages during osteoclast differentiation. Suppression by selective gamma-secretase inhibitor or short hairpin RNA suppresses RANKL-induced osteoclastogenesis. contrast, induction ectopic intracellular enhances...
Abstract Osteoclast differentiation is critically dependent on calcium (Ca2+) signaling. Transient receptor potential vanilloid 4 (TRPV4), mediates Ca2+ influx in the late stage of osteoclast and thereby regulates However, system-modifying effect TRPV4 activity remains to be determined. To elucidate mechanisms underlying activation based differentiation, gain-of-function mutants were generated by amino acid substitutions R616Q V620I introduced into lineage Trpv4 null mice generate...
Although important roles of receptor activator NF‐κB ligand (RANKL) and its (RANK) have been established for osteoclastogenesis bone resorption, their expression during physiological root resorption remain uncertain. Physiological shedding human deciduous teeth is mediated by osteoclast‐like cells (odontoclasts). In this study, we examined the RANKL osteoprotegerin (OPG), a decoy that prevents from binding to RANK in periodontal ligament (PDL) using immunocytochemistry reverse transcriptase...
We identified a previously unknown integrin, alpha(9)beta(1), on OCLs and their precursors. Antibody to alpha(9) inhibited OCL formation in human marrow cultures, from knockout mice had defect actin ring reorganization an impaired bone resorption capacity.Integrins play important roles osteoclast (OCL) function. Mature mainly express alpha(v)beta(3) heterodimer adhesion receptor that has been implicated osteoclastic resorption. ADAM8, disintegrin metalloproteinase, as novel stimulator of...
We investigated the transport of salicylic acid and L-lactic across placenta using human trophoblast cell line BeWo. performed uptake experiments measured change in intracellular pH (pH(i)). The uptakes [(14)C]salicylic L-[(14)C]lactic were temperature- extracellular pH-dependent saturable at higher concentrations. Both also reduced by FCCP, nigericin, NaN(3). Various nonsteroidal anti-inflammatory drugs (NSAIDs) strongly inhibited acid. Salicylic ibuprofen noncompetitively...
Transient receptor potential (TRP) ion channels are critical contributors to the perception of various environmental stimuli. Although oral cavity is access point for food types, expression TRP in mucosa remains unknown. We hypothesized that epithelium itself may participate sensing thermal, mechanical, and chemical conditions. The profiles exhibited regional differences among buccal, palatal, tongue epithelia. Changes elevated intracellular Ca 2+ concentration ([Ca ] i ) epithelial cells...
Excessive mechanical stress (MS) during hyperocclusion is known to result in disappearance of the alveolar hard line, enlargement periodontal ligament (PDL) space, and destruction bone, leading occlusal traumatism. We hypothesized that MS induces expression osteoclastogenesis-associated chemokines PDL tissue, resulting chemotaxis osteoclastogenesis examined effect on relationships between chemokine using vivo vitro models. In an model, intermittent stretching-induced was shown up-regulate CC...
Abstract Purpose The acquisition of osseointegration during implant therapy is slower and poorer in patients with diabetes compared healthy persons. serum concentration adiponectin type II lower than that persons via the suppression AMP-activated protein kinase (AMPK). Therefore, we hypothesized AMPK activation enhances bone formation around implants, resulting improved osseointegration. purpose this study was to evaluate impact on osteoblast differentiation its mechanism downstream...
ClC-7 Cl(-) channels expressed in osteoclasts are important for bone resorption since it has been shown that disruption of the ClCN7 gene mice leads to severe osteopetrosis. We have previously reported currents recorded from mouse resemble those ClC-3 channels. The aim present study was determine expression and their functional role during resorption. detected transcripts both by RT-PCR. confirmed immunocytochemical staining. Mouse lacking (ClC-3(-/-) osteoclasts) derived ClCN3...
Programmed cell death ligands (PD-Ls) are expressed in tumor cells where they bind to programmed death-1, an immunocyte co-receptor, resulting evasion from the immune system. Chemotherapeutic drugs have been recently reported induce expression of PD-L, such as PD-L1, some cancer cells. However, little is known regarding PD-L2 and its role oral squamous carcinoma (OSCC). In this study, we examined effect cisplatin on regulation OSCC lines analyzed malignant behavior PD-L2-expressing using...
Periodontal ligament (PDL) cells play important roles in root resorption of human deciduous teeth by odontoclasts (osteoclast-like cells). However, it is unclear how PDL regulate osteoclastogenesis. We examined the effects PTHrP, TGF-β, and EGF, which are all secreted tooth germ, on tartrate-resistant acid-phosphatase-positive (TRAP + ) cell formation using co-cultures mouse spleen cells. Only PTHrP promoted TRAP co-cultures. induced receptor activator NF-κB ligand (RANKL) mRNA expression...
The plasma membrane Na(+)/Ca(2+) exchanger (NCX) is a bidirectional transporter that mediates the exchange of Na(+) for Ca(2+) depending on electrochemical gradients. Mammalian NCXs form multigene family comprising NCX1, NCX2, and NCX3 isoforms. Although it has been known NCX1 in rat osteoclasts coupled with Na(+)/ H(+) regulation intracellular concentration ([Ca(2+)](i)), unclear what kind variants are expressed whether other two NCX isoforms also present mouse osteoclasts. To clarify role...
Periodontal ligament (PDL) cells are known to play important roles in tooth eruption and alveolar bone metabolism. We previously reported that PTHrP increases RANKL expression human PDL cells, suggesting it promotes odontoclastic root resorption during eruption. While is Notch-related genes a key role development, the of Notch signaling pathway less clear. hypothesized induces ligand thereby regulates osteo- odontoclastogenesis. found increased Notch1 Jagged1 dose- time-dependent manner....
Purpose: The bone regenerative healing process is often prolonged, with a high risk of infection particularly in elderly and diseased patients. A reduction time usually requires mechanical stress devices, chemical cues, or laser/thermal therapies. Although these approaches have been used extensively for the time, exact mechanisms involved thermal stress-induced regeneration remain unclear. Methods: Photothermal (PTS) stimulation was carried out using novel photothermal device, composed an...
Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is likely to be caused by continuous imperfection bone healing after surgical treatments in patients with long-term administration nitrogen-containing bisphosphonates (NBPs). NBPs inhibit osteoclastic resorption impairing mevalonic acid sterol pathway osteoclasts. Thus, we hypothesized that exogenous metabolites restore inhibitory effects on osteoclastogenesis and remodeling. To clarify metabolites, especially geranylgeranyl...
The bone regenerative healing process is often prolonged, with a high risk of infection particularly in elderly and diseased patients. A reduction time usually requires mechanical stress devices, chemical cues, or laser/thermal therapies. Although these approaches have been used extensively for the time, exact mechanisms involved thermal stress-induced regeneration remain unclear. In this study, we investigated effect optimal hyperthermia on rat calvarial defects vivo osteogenesis vitro....