A5006647628- Bone Metabolism and Diseases
- Bone health and treatments
- Phase-change materials and chalcogenides
- NF-κB Signaling Pathways
- Immune Response and Inflammation
- dental development and anomalies
- Orthodontics and Dentofacial Orthopedics
- Mycobacterium research and diagnosis
- Semiconductor Lasers and Optical Devices
- Tuberculosis Research and Epidemiology
- Bone health and osteoporosis research
- Cell Adhesion Molecules Research
- Inflammatory mediators and NSAID effects
- Oral microbiology and periodontitis research
- Endodontics and Root Canal Treatments
- Bone and Dental Protein Studies
- Cytokine Signaling Pathways and Interactions
- Bone and Joint Diseases
- Dental materials and restorations
- Osteomyelitis and Bone Disorders Research
- Liquid Crystal Research Advancements
- Biomarkers in Disease Mechanisms
- Peptidase Inhibition and Analysis
- Temporomandibular Joint Disorders
- Connective Tissue Growth Factor Research
Tohoku University
2016-2025
Nagasaki University
2000-2011
Panasonic (Japan)
1998-2008
Washington University in St. Louis
2005-2008
Tokyo Medical and Dental University
2008
University of Missouri–St. Louis
2007
Osaka University
1996-2006
American Association of Orthodontists
2006
Osaka Health Science University
2006
Okayama University
2005
TNF-induced receptor activator NF-kappaB ligand (RANKL) synthesis by bone marrow stromal cells is a fundamental component of inflammatory osteolysis. We found that this process was abolished IL-1 antagonist (IL-1Ra) or in derived from type I receptor-deficient (IL-1RI-deficient) mice. Reflecting sequential signaling the cytokines TNF and IL-1, induces cell expression IL-1RI. These data suggest regulates RANKL via and, therefore, plays role periarticular Consistent with posture,...
TNF-induced receptor activator NF-κB ligand (RANKL) synthesis by bone marrow stromal cells is a fundamental component of inflammatory osteolysis. We found that this process was abolished IL-1 antagonist (IL-1Ra) or in derived from type I receptor–deficient (IL-1RI–deficient) mice. Reflecting sequential signaling the cytokines TNF and IL-1, induces cell expression IL-1RI. These data suggest regulates RANKL via and, therefore, plays role periarticular Consistent with posture, TNF-stimulated...
The pathogenesis of glucocorticoid-induced (GC-induced) bone loss is unclear. For example, osteoblast apoptosis enhanced by GCs in vivo, but they stimulate formation vitro. This conundrum suggests that an intermediary cell transmits a component the bone-suppressive effects to osteoblasts intact animal. Bone remodeling characterized tethering activities osteoclasts and osteoblasts. Hence, osteoclast potential modulator effect on To define direct impact bone-resorptive cells, we compared...
Osteoimmunology peeks into the interaction of bone and immune system, which has largely proved to be a multiplex reaction. Osteocytes have been shown regulate resorption through expression RANKL in physiologic pathologic conditions. TNF-α, product is an important cytokine regulating inflammatory conditions either directly or by increasing M-CSF expressions osteoblasts stromal cells. The effect TNF-α on wide range cell types documented; however, direct osteocytes not established yet. In this...
Osteoclasts are multinucleated cells that differentiate from hematopoietic and possess characteristics responsible for bone resorption. To study the involvement of mitogen-activated protein kinases (MAPKs) in osteoclastogenesis murine monocytic cell line RAW264.7, which can into osteoclast-like presence receptor activator nuclear factor kappa B ligand (RANKL), we treated with specific inhibitors p38 MAPK, PD169316 SB203580, MAPK extracellular signaling-regulated kinase (ERK) (MEK), U0126...
TNF-alpha is the dominant cytokine in inflammatory osteolysis. Using mice whose BM stromal cells and osteoclast precursors are chimeric for presence of TNF receptors, we found that both cell types mediated cytokine's osteoclastogenic properties. The greater contribution was made, however, by express M-CSF. stimulated M-CSF gene expression, vivo, only TNF-responsive cells. M-CSF, turn, induced key receptor, receptor activator NF-kappaB (RANK), precursors. In keeping with proproliferative...
In this study, we establish that the tyrosine kinase Syk is essential for osteoclast function in vitro and vivo. Syk−/− osteoclasts fail to organize their cytoskeleton, and, as such, bone-resorptive capacity arrested. This defect results increased skeletal mass embryos dampened basal stimulated bone resorption chimeric mice whose lack kinase. The impact of deficiency reflects diminished activity mature not impaired differentiation. regulates by its inclusion with αvβ3 integrin c-Src a...
Abstract Runx2 is an essential transcription factor for osteoblast differentiation. However, the functions of in postnatal bone development remain to be clarified. Introduction dominant‐negative (dn)‐ did not inhibit Col1a1 and osteocalcin expression mature osteoblastic cells. In transgenic mice that expressed dn‐ osteoblasts, trabecular had increased mineralization, volume, features compact bone, major matrix protein genes was relatively maintained. After ovariectomy, neither osteolysis nor...
Abstract The marrow stromal cell is the principal source of key osteoclastogenic cytokine receptor activator NF-κB (RANK) ligand (RANKL). To individualize role cells in varying states TNF-α-driven osteoclast formation vivo, we generated chimeric mice which wild-type (WT) marrow, immunodepleted T and cells, transplanted into lethally irradiated deleted both p55 p75 TNFR. As control, similarly treated WT was mice. Each group administered increasing doses TNF-α. Exposure to high-dose ex vivo...
Osteoclasts (OCs) function to reabsorb bone and are responsible for the loss associated with inflammatory arthritis osteoporosis. OC numbers elevated in most disorders of accelerated destruction, reflecting altered rates precursor differentiation apoptosis. Both these processes regulated by JNK family MAP kinases. In this study, we have demonstrated that NF-kappaB subunit RelA/p65 inhibits JNK-mediated apoptosis during a critical period commitment phenotype response cytokine RANKL. This...
Objective To clarify the function of osteoclast‐like multinuclear cells differentiated from bone marrow–derived macrophages (BMMs) by a combination tumor necrosis factor α (TNFα) and interleukin‐6 (IL‐6), to investigate molecular mechanisms underlying differentiation. Methods BMMs were stimulated TNFα and/or IL‐6. The then compared with conventional osteoclasts in vitro RANKL. An pit formation assay on dentine slices an vivo resorption calvarial bones performed. We also evaluated activities...
Lipopolysaccharide (LPS) is related to osteoclastogenesis in osteolytic diseases. Interleukin- (IL-) 12 an inflammatory cytokine that plays a critical role host defense. In this study, we investigated the effects of IL-12 on LPS-induced osteoclastogenesis. LPS was administered with or without into supracalvariae mice, and alterations calvarial suture were evaluated histochemically. The number osteoclasts mRNA level tartrate-resistant acid phosphatase (TRAP), osteoclast marker, lower mice...
Abstract Osteoclast differentiation is critically dependent on calcium (Ca2+) signaling. Transient receptor potential vanilloid 4 (TRPV4), mediates Ca2+ influx in the late stage of osteoclast and thereby regulates However, system-modifying effect TRPV4 activity remains to be determined. To elucidate mechanisms underlying activation based differentiation, gain-of-function mutants were generated by amino acid substitutions R616Q V620I introduced into lineage Trpv4 null mice generate...
It is well known that the moderately roughened surfaces of dental implants enhance direct bone-implant contact. However, rough implant surfaces, as compared to smooth are thought pose a higher risk bacterial infection when exposed oral cavity.This study was focused on evaluating photocatalytic bactericidal effects anatase titanium dioxide (TiO(2)) gram-negative anaerobic bacteria be associated with periimplantitis.A film TiO(2) added onto surface commercially pure disks by plasma source ion...
NF-(kappa)B is an important component of both autoimmunity and bone destruction in RA. NF-(kappa)B-inducing kinase (NIK) a key mediator the alternative arm pathway, which characterized by nuclear translocation RelB/p52 complexes. Mice lacking functional NIK have no peripheral lymph nodes, defective B T cells, impaired receptor activator NF-kappaB ligand-stimulated osteoclastogenesis. We investigated role murine models inflammatory arthritis using Nik-/- mice. The serum transfer model...
We have demonstrated for the first time feasibility of using a rewritable dual-layer phase-change optical disk utilizing blue-violet laser. For medium, we adopted very thin recording layer with new material Ge–Sn–Sb–Te, and 10-nm-thick silver-alloy reflective to obtain large transmittance high-quality signals. second optimized thickness each both absorption small heat capacity. Carrier-to-noise ratios more than 50 dB, erasability 30 dB powers 8 mW medium 11 were obtained under typical...
Mechanical stress such as orthodontic tooth movement induces osteoclastogenesis. Sometimes, excessive mechanical results in root resorption during movement. It has been reported that bisphosphonate inhibits Recently, there have concerns for patients receiving bisphosphonates. Thus, the aim of this study was to investigate effect bisphosphonates on and mice. A nickel-titanium (Ni-Ti) closed coil spring delivering a force 10 g inserted between upper anterior alveolar bone first molar...
Bcl2 subfamily proteins, including and Bcl-XL, inhibit apoptosis. As osteoblast apoptosis is in part responsible for osteoporosis sex steroid deficiency, glucocorticoid excess, aging, bone loss might be inhibited by the upregulation of Bcl2; however, effects overexpression on differentiation development maintenance have not been fully investigated. To investigate these issues, we established two lines osteoblast-specific BCL2 transgenic mice. In mice, volume was increased at 6 weeks age but...