A5008153918- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Dietary Effects on Health
- Biochemical Analysis and Sensing Techniques
- Stress Responses and Cortisol
- Neuropeptides and Animal Physiology
- Tryptophan and brain disorders
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroendocrine regulation and behavior
- Folate and B Vitamins Research
- Pharmacological Effects and Assays
- Thyroid Disorders and Treatments
- Alcohol Consumption and Health Effects
- Electron Spin Resonance Studies
- Circadian rhythm and melatonin
- Memory and Neural Mechanisms
- Biochemical effects in animals
- Analytical Chemistry and Sensors
- Growth Hormone and Insulin-like Growth Factors
- Spectroscopy Techniques in Biomedical and Chemical Research
- Attention Deficit Hyperactivity Disorder
- Estrogen and related hormone effects
University of Cincinnati
2003-2024
University of Cincinnati Medical Center
2016
Sabin Vaccine Institute
2009
Florida State University
2000-2003
Harlow College
1999
Ealing Hospital
1998
Ealing Hospital NHS Trust
1998
We utilized variations in caloric availability and ambient temperature (T(a)) to examine interrelationships between energy expenditure cardiovascular function mice. Male C57BL/6J mice (n = 6) were implanted with telemetry devices housed metabolic chambers for measurement of mean arterial pressure (MAP), heart rate (HR), O(2) consumption (VO(2)), locomotor activity. Fasting (T(a) 23 degrees C), initiated at the onset dark phase, resulted large transient depressions MAP, HR, VO(2), activity...
The second-largest cause of X-linked mental retardation is a deficiency in creatine transporter (CRT; encoded by SLC6A8), which leads to speech and language disorders with severe cognitive impairment. This syndrome, caused the absence brain, currently untreatable because CRT required for entry into brain cells. Here, we developed brain-specific Slc6a8 knockout mouse (Slc6a8-/y) as an animal model human order explore potential therapies this syndrome. phenotype Slc6a8-/y was comparable that...
Leptin regulates energy balance and glucose homeostasis, at least in part, via activation of receptors the arcuate nucleus hypothalamus located proopiomelanocortin (POMC) neurons. Females have greater sensitivity to central leptin than males, suggested by a anorectic effect administration females. We hypothesized that regulation peripheral homeostasis female rodents would be affected extent males if action POMC neurons were disturbed. Male mice lacking only gained significantly more body...
CCK acts peripherally as a satiating peptide released during meals in response to lipid feeding and centrally functions the modulation of feeding, exploratory, memory activities. The present study determined metabolic parameters, food intake, anxiety-like behaviors, cognitive function mice lacking gene. We studied intestinal fat absorption, body composition, intake knockout (CCK-KO) by using noninvasive measurement absorption along with quantitative magnetic resonance (QMR) imaging DietMax...
The primary purpose of the study was to test hypothesis that reduced leptin signaling is necessary elicit cardiovascular and metabolic responses fasting. Lean ( Fa/?; normal receptor; n = 7) obese fa/fa; mutated 8) Zucker rats were instrumented with telemetry transmitters housed in chambers at 23°C (12:12-h light-dark cycle) for continuous (24 h) measurement variables. Before fasting, mean arterial pressure (MAP) higher (MAP: 103 ± 3; lean 94 1 mmHg), whereas oxygen consumption (V˙o 2 : 16.5...
1. In the present study, we determined effect of diet-induced obesity on cardiovascular and metabolic regulation in mice at standard laboratory temperatures (ambient temperature (Ta) = 22 degrees C) during exposure to thermoneutrality (Ta 30 C). 2. Male C57BL/6J (B6) fed a high-fat diet (HFF; n 17) or chow (CHW; 14) for 15 weeks were surgically instrumented with telemetry devices, housed chambers assigned either control atenolol treatment (25 mg/kg per day drinking water) determine effects...
Abstract Psychological loss is a common experience that erodes well-being and negatively impacts quality of life. The molecular underpinnings are poorly understood. Here, we investigate the mechanisms using an environmental enrichment removal (ER) paradigm in male rats. basolateral amygdala (BLA) was identified as region interest, demonstrating differential Fos responsivity to ER having established role stress processing adaptation. A comprehensive multi-omics investigation BLA, spanning...
Fasting produces multiple cardiovascular, metabolic, and behavioral responses. To examine the interrelationship between these responses, male spontaneously hypertensive rats (SHR; n = 8) implanted with cardiovascular telemetry devices were housed in metabolic chambers at 23°C for 22-h daily measurements of physiological variables. The experimental apparatus was designed so that ingestive behavior detected by photobeams locomotion a load sensor. Cardiovascular status determined as both...
The role of reduced leptin signaling in the regulation cardiovascular responses to negative energy balance is not known. We tested hypothesis that central infusion would attenuate and metabolic fasting. Male Sprague-Dawley rats, instrumented with telemetry devices intracerebroventricular cannulas, were housed chambers for continuous (24 hours) measurement dark-phase (active) light-phase (inactive) mean arterial pressure, heart rate, oxygen consumption, respiratory quotient. Rats received...
Chronic stress leads to hypofunction of the medial prefrontal cortex (mPFC), mechanisms which remain be determined. Enhanced activation GABAergic parvalbumin (PV) expressing interneurons (INs) is thought play a role in stress-induced inhibition. In this study, we tested whether chemogenetic inhibition mPFC PV INs after chronic can rescue stress-related behavioral and physiological phenotypes. Mice underwent 2 weeks variable (CVS) followed by battery tests known affected exposure, e.g. an...
Challenges to energy homeostasis, such as cold exposure, can have consequences for both metabolic and cardiovascular functioning. We hypothesized that 1-wk exposure (4°C) would produce concurrent increases in rate (V˙o 2 ; indirect calorimetry), heart (HR), mean arterial blood pressure (MAP) measured by telemetry. In the initial hours of change ambient temperature (T a ), spontaneously hypertensive rats (SHRs) normotensive Sprague-Dawley showed rapid (in cold) or decreases rewarming) V˙o ,...
The melanin-concentrating hormone-1 receptor (MCH1R) is a G-protein-coupled expressed in the brain and peripheral tissues that regulates energy storage body weight. Here, we focused on discovery of mechanism site action for small-molecule MCH1R antagonist, which yields weight loss mouse model human obesity. throughout but also found known to regulate fat utilization, e.g., skeletal muscle adipose tissue. Previous studies antagonist have not delineated critical mediating anorexigenic...
Objective Nausea and aversive food responses are commonly reported following bariatric surgery, along with post‐surgical reduction in meal size. This study investigates whether a size limit can be conditioned by associating large meals outcomes. Methods In rats, the intake of exceeding pre‐defined threshold was paired lithium chloride‐induced gastric illness, effects on self‐determined intakes body weight were measured. Results Rats given LiCl contingent learned to reliably reduce below this...
ABSTRACT Post-Traumatic Stress Disorder (PTSD) is a debilitating condition in which traumatic experience triggers symptoms related to re-experiencing, avoidance, arousal, and mood dysregulation. PTSD negatively impacts 6% of people during their lifetime, with women being disproportionally affected exhibiting different, more severe than men. Despite this widespread impact, the molecular mechanisms underlying its sex differences remain poorly understood. Pituitary Adenylate Cyclase-Activating...
ABSTRACT Psychological loss is a common experience that erodes well-being and negatively impacts quality of life. The molecular underpinnings are poorly understood. Here, we investigate the mechanisms using an enrichment removal (ER) paradigm in rats. A comprehensive multi-omics investigation basolateral amygdala (BLA) revealed alterations microglia extracellular matrix (ECM). Follow-up studies indicated ER decreased size, complexity, phagocytosis, suggesting reduced immune surveillance....
65 Caloric restriction is commonly prescribed to obese individuals reduce their risk for cardiovascular disease. Hypothalamic pathways regulated by leptin are important in activating homeostatic responses reduced caloric intake. We tested the hypothesis that central infusion of would prevent reductions blood pressure (BP), heart rate (HR), and oxygen consumption (VO 2 ) produced acute deprivation. Male Sprague-Dawley rats were instrumented with telemetry devices measuring icv guide cannulae...
65 Caloric restriction is commonly prescribed to obese individuals reduce their risk for cardiovascular disease. Hypothalamic pathways regulated by leptin are important in activating homeostatic responses reduced caloric intake. We tested the hypothesis that central infusion of would prevent reductions blood pressure (BP), heart rate (HR), and oxygen consumption (VO 2 ) produced acute deprivation. Male Sprague-Dawley rats were instrumented with telemetry devices measuring icv guide cannulae...