A5019791733- Trypanosoma species research and implications
- Biochemical and Molecular Research
- Research on Leishmaniasis Studies
- Cancer survivorship and care
- Health Systems, Economic Evaluations, Quality of Life
- Traumatic Brain Injury and Neurovascular Disturbances
- Magnetic properties of thin films
- Global Cancer Incidence and Screening
- Cardiomyopathy and Myosin Studies
- Musculoskeletal pain and rehabilitation
- Spine and Intervertebral Disc Pathology
- Cerebrovascular and Carotid Artery Diseases
- Clinical practice guidelines implementation
- Single-cell and spatial transcriptomics
- Cerebrospinal fluid and hydrocephalus
- Healthcare cost, quality, practices
- Theoretical and Computational Physics
- Gene Regulatory Network Analysis
- Insect symbiosis and bacterial influences
- CRISPR and Genetic Engineering
- COVID-19 and Mental Health
- RNA modifications and cancer
- Radiopharmaceutical Chemistry and Applications
- Gene expression and cancer classification
- Monoclonal and Polyclonal Antibodies Research
Humboldt-Universität zu Berlin
2005-2024
Charité - Universitätsmedizin Berlin
2005-2024
Freie Universität Berlin
2016-2024
University of Basel
2011
University of Regensburg
2008-2009
Heidelberg University
1994-2008
Council for Jewish Elderly
2008
Ludwig-Maximilians-Universität München
2005-2006
DKFZ-ZMBH Alliance
2005
University of Tübingen
1997-1999
Background and Purpose Cerebrovascular CO 2 reactivity can be assessed easily reliably by transcranial Doppler sonography. The objectives of the present study were to evaluate sex differences in cerebral specify relation between blood flow velocity. Methods circulation both middle arteries was measured bilateral sonography 60 healthy volunteers (30 men, 30 women) aged 21 58 years. End-tidal carbon dioxide tensions (P etco ) elevated with use carbogene gas (95% O , 5% ). In each subject mean...
Static magnetic and spin wave properties of square lattices permalloy micron dots with thicknesses 500 1000 Å varying dot separations have been investigated. A fourfold anisotropy was found for the lattice diameters 1 μm a separation 0.1 μm. The is attributed to an anisotropic dipole–dipole interaction between magnetically unsaturated parts dots. strength (order 105 erg/cm3) decreases increasing in-plane applied field.
African trypanosomes are extracellular protozoan parasites that transmitted from one mammalian host to the next by tsetse flies. Bloodstream forms express variant surface glycoprotein (VSG); fly (procyclic) instead procyclic acidic repetitive protein (PARP). PARP mRNA is abundant in and almost undetectable blood-stream forms. Post-transcriptional mechanisms mainly responsible for regulation but results of nuclear run-on experiments suggested transcription might also be regulated. We measured...
Messenger RNAs of higher eukaryotes share a functionally essential 5′ monomethyl CAP structure generated during reaction that is linked exclusively to RNA polymerase II transcription. In unicellular parasites belonging the Kinetoplastida, however, mRNAs acquire their through trans-splicing which effectively uncouples pol transcription and capping. Consequently functional can be produced by endogenous I. Here we demonstrate extension this flexibility heterologous bacteriophage polymerases....
Nearly all trypanosome mRNAs are synthesized as polycistronic precursors, from which mature excised by trans splicing and polyadenylation. Polyadenylation of a procyclic acidic repetitive protein (PARP, or procyclin) transcript was studied transient transfection constructs bearing chloramphenicol acetyltransferase gene linked to the PARP intergenic region. usually occurred at A residues, about 100 bases upstream trans-splicing acceptor signal. The wild-type polyadenylation site has cryptic...
Over the last decade, important insights into regulation of cellular responses to various stimuli were gained by global gene expression analyses cell populations. More recently, specific functions and underlying regulatory networks rare cells isolated from their natural environment moved center attention. However, low numbers still hinder profiling ex vivo material in biomedical research. Therefore, we developed a robust method for single on high-density oligonucleotide arrays with excellent...
In the African trypanosome Trypanosoma brucei nearly all control of gene expression is posttranscriptional; sequences in 3'-untranslated regions mRNAs determine steady-state mRNA levels by regulation RNA turnover. Here we investigate roles two related proteins, TbUBP1 and TbUBP2, containing a single recognition motif, expression. TbUBP2 are cytoplasm nucleus, comprise ca. 0.1% total protein, not associated with polysomes or degradation enzymes. Overexpression upregulated several potentially...
Background: Collecting patient-reported outcome (PRO) data systematically enables objective evaluation of treatment and its related outcomes. Using disease-specific questionnaires developed by the International Consortium for Health Outcome Measurement (ICHOM) allows comparison between physicians, hospitals, even different countries. Objective: This pilot project aimed to establish a digital system measure PROs new patients with breast cancer who attended Charité Breast Center approach...
There is still some controversy about alterations in velocity of blood flow and cerebral vasomotor reactivity intracranial arteries migraineurs during the interictal phase. By means simultaneous bilateral transcranial Doppler ultrasonography we, therefore, assessed fIow velocities cerebrovascular to carbon dioxide all three basal brain 20 phase 30 nonheadache‐prone control subjects. Mean were higher than controls on both sides, with a significant difference ( P <0.05) for right anterior...
RNA interference (RNAi) in Trypanosoma brucei was first reported 1998 (1). As other eukaryotes, involves digestion of the interfering double-stranded into short fragments (2), a polysome-associated complex (3), and Argonaute protein (4,5). T. is an ideal organism for testing gene function by RNAi. A complete genome sequence available, liquid suspension culture unproblematic. Various combinations RNAi vector host trypanosomes, with different advantages, are available; if procedures working...
The assessment of health-related quality life (HRQOL) measured via patient-reported outcomes (PROs) is a key component in clinical trials and increasingly used routine worldwide. Two PRO measures (PROMs) that share the same definition health report on comparable T-metric anchored to general population samples are PROMIS-29 EORTC QLQ-C30. In this study, we investigate empirical agreement these underlying concepts.We collected QLQ-C30 data from 1,478 female patients at breast cancer outpatient...
Abstract Background The interpretation of patient-reported outcomes requires appropriate comparison data. Currently, no patient-specific reference data exist for the Patient-Reported Outcome Measurement Information System (PROMIS) Physical Function (PF), Upper Extremity (UE), and Pain Interference (PI) scales individuals 50 years older. Questions/purposes (1) Can all PROMIS PF, UE, PI items be used valid cross-country comparisons in these domains among United States, Kingdom, Germany? (2)...
Nearly all trypanosome mRNAs are synthesized as polycistronic precursors, from which mature excised by trans splicing and polyadenylation. Polyadenylation of a procyclic acidic repetitive protein (PARP, or procyclin) transcript was studied transient transfection constructs bearing chloramphenicol acetyltransferase gene linked to the PARP intergenic region. usually occurred at A residues, about 100 bases upstream trans-splicing acceptor signal. The wild-type polyadenylation site has cryptic...