A5091768289- Cancer-related molecular mechanisms research
- Prostate Cancer Treatment and Research
- RNA Research and Splicing
- Estrogen and related hormone effects
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Molecular Biology Techniques and Applications
- Cardiac Fibrosis and Remodeling
- Peroxisome Proliferator-Activated Receptors
- Cardiovascular Function and Risk Factors
- Mitochondrial Function and Pathology
- Hormonal Regulation and Hypertension
- Heat shock proteins research
- Coenzyme Q10 studies and effects
- Telomeres, Telomerase, and Senescence
- Cholesterol and Lipid Metabolism
- Metabolism and Genetic Disorders
- Extracellular vesicles in disease
- Thyroid Cancer Diagnosis and Treatment
- Nuclear Structure and Function
- Glutathione Transferases and Polymorphisms
- Congenital heart defects research
- Skin and Cellular Biology Research
- Vitamin D Research Studies
- Cardiac pacing and defibrillation studies
Istituto di Analisi dei Sistemi ed Informatica Antonio Ruberti
2020-2024
National Research Council
2011-2024
Consorzio Roma Ricerche
2023-2024
Università Cattolica del Sacro Cuore
2018-2021
Institute of Cell Biology and Neurobiology
2011-2019
National Cancer Institute
2012
Catholic University of America
2008-2012
Institute of Neurobiology and Molecular Medicine
2008-2010
Magna Graecia University
2006-2010
Istituto Dermopatico dell'Immacolata
2008
The identification of biomarkers that distinguish between aggressive and indolent forms prostate cancer (PCa) is crucial for diagnosis treatment. In this study, we used cultured cells derived from tissue patients with PCa to define a molecular mechanism underlying the most form involves functional activation eNOS HIFs in association estrogen receptor β (ERβ). Cells poor prognosis exhibited constitutively hypoxic phenotype increased NO production. Upon treatment, formation ERβ/eNOS,...
Background Hypoxia inducible factor-1α (HIF-1α) is responsible for the majority of HIF-1-induced gene expression changes under hypoxia and "angiogenic switch" during tumor progression. HIF-1α often upregulated in tumors leading to more aggressive growth chemoresistance, therefore representing an important target antitumor intervention. We previously reported that zinc downregulated levels. Here, we evaluated molecular mechanisms zinc-induced downregulation whether affected also vivo....
Abstract In the complex network of nuclear hormone receptors, long non-coding RNAs (lncRNAs) are emerging as critical determinants action. Here we investigated involvement selected cancer-associated lncRNAs in Estrogen Receptor (ER) signaling. Prior studies by Chromatin Immunoprecipitation (ChIP) Sequencing showed that prostate cancer cells ERs form a with endothelial nitric oxide synthase (eNOS) and turn these complexes associate chromatin an estrogen-dependent fashion. Among associations...
Abstract Extracellular vesicles (EVs) and their cargo represent an intriguing source of cancer biomarkers for developing robust sensitive molecular tests by liquid biopsy. Prostate (PCa) is still one the most frequent deadly tumor in men analysis EVs from biological fluids PCa patients has proven feasibility unprecedented potential such approach. Here, we exploited antibody-based proteomic technology, i.e. Reverse-Phase Protein microArrays (RPPA), to measure key antigens activated signaling...
Anaplastic thyroid cancer (ATC) is an extremely aggressive tumor characterized by marked epithelial mesenchymal transition, which leads, almost invariably, to death. Peroxisomal proliferator-activated receptor (PPAR)-gamma agonists have recently emerged as potential antineoplastic drugs. To establish whether ATC could be a target of PPAR gamma agonists, we first examined protein expression in panel six cell lines and then studied the biologic effects two ciglitazone rosiglitazone, that...
We report that in endothelial cells, the angiogenic effect of 17beta-estradiol (E2) is inhibited by estrogen receptor (ER) antagonist ICI or NO synthase (NOS) inhibitor 7-nitroindazole via downregulation hTERT, telomerase catalytic subunit, suggesting E2 and are involved controlling hTERT transcription. Quantitative Real-Time PCR chromatin immunoprecipitations E2-treated human umbilical vein showed recruitment ERs on promoter concomitant enrichment histone 3 methylation at Lysine 79, a...
Abstract Background About 30% of Prostate cancer (PCa) patients progress to metastatic PCa that remains largely incurable. This evidence underlines the need for development innovative therapies. In this direction, potential research focus might be on long non-coding RNAs (lncRNAs) like H19, which serve critical biological functions and show significant dysregulation in cancer. Previously, we showed a transcriptional down-regulation H19 under combined pro-tumoral estrogen hypoxia treatment...
Vimentin, a mesenchymal marker, is frequently overexpressed in epithelial carcinomas undergoing to transition (EMT), condition correlated with invasiveness and poor prognosis. Therefore, vimentin potential molecular target for anticancer therapy. Emerging studies experimental models underscore the functions of homeodomain-interacting protein kinase 2 (HIPK2) as oncosuppressor by acting transcriptional corepressor or catalytic activator molecules involved apoptosis response antitumor drugs....
Estrogen and hypoxia promote an aggressive phenotype in prostate cancer (PCa), driving transcription of progression-associated genes. Here, we molecularly dissect the contribution long non-coding RNA H19 to PCa metastatic potential under combined stimuli, a topic largely uncovered. The effects estrogen on cell adhesion molecules' expression were investigated cells PCa-derived organotypic slice cultures (OSCs) by qPCR Western blot. molecular mechanism was addressed chromatin...
In presence of indeterminate lesions by fine needle aspiration (FNA), thyroid cancer cannot always be easily diagnosed conventional cytology. As a consequence, unnecessary removal gland is performed in patients without based on the lack optimized diagnostic criteria. Aim this study identifying molecular profile long noncoding RNAs (lncRNAs) expression capable to discriminate between benign and malignant nodules.Patients were subjected surgery (n = 19) for cytologic suspicious nodules or FNA...
We recently identified in prostate tumors (PCa) a transcriptional prognostic signature comprising significant number of genes differentially regulated patients with worse clinical outcome. Induction up-regulated was due to chromatin remodeling by combinatorial complex between estrogen receptor (ER)-β and endothelial nitric oxide synthase (eNOS). Here we show that this can also repress transcription are down-regulated PCa, such as the glutathione transferase gene GSTP1. Silencing GSTP1 is...
This study aims at investigating the epigenetic landscape of cardiomyocytes exposed to elevated glucose levels. High (30 mM) for 72 hours determined some changes in mouse HL-1 and rat differentiated H9C2 including upregulation class I III histone deacetylase protein levels activity, inhibition acetylase p300 increase H3 lysine 27 trimethylation, reduction 9 acetylation. Gene expression analysis focused on cardiotoxicity revealed that high induced markers associated with tissue damage,...
In previous work we have documented the nuclear translocation of endothelial NOS (eNOS) and its participation in combinatorial complexes with Estrogen Receptor Beta (ERβ) Hypoxia Inducible Factors (HIFs) that determine localized chromatin remodeling response to estrogen (E2) hypoxia stimuli, resulting transcriptional regulation genes associated adverse prognosis prostate cancer (PCa). To explore role eNOS acquisition aggressive phenotype PCa, performed ChIP-Sequencing on chromatin-associated...
Abstract Here, with the aim of obtaining insight into intriguing selectivity G-quadruplex (G4) ligands toward cancer compared to normal cells, a genetically controlled system progressive transformation in human BJ fibroblasts was analyzed. Among different comparative evaluations, we found increase DNA damage response (DDR) markers throughout genome from immortalized and transformed cells. More interestingly, sensitivity G4 strongly correlated presence basal level damage, including at...
Nucleoporin 153 (Nup153), key regulator of nuclear import/export, has been recently associated to oncogenic properties in pancreatic and breast tumour cells modulating either cell motility migration or gene expression by chromatin association. In the present work, we have characterized role Nup153 a cellular model prostate cancer (PCa). The analysis several immortalized lines derived from freshly explants specimens showed that protein was higher multimeric complexes with eNOS ERβ as compared...
The lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) promotes growth and progression in prostate cancer (PCa); however, little is known about its possible impact PCa metabolism. aim of this work has been the assessment metabolic reprogramming associated with MALAT1 silencing human cells an ex vivo model organotypic slice cultures (OSCs). Cultured OSCs derived from primary tumors were transfected specific gapmers. Cell survival, gene profiling, evaluation targeted...
Choline kinase alpha (CHKA), an essential gene in phospholipid metabolism, is among the modulated MALAT1-targeted transcripts advanced and metastatic prostate cancer (PCa).We analyzed CHKA mRNA by qPCR upon MALAT1 targeting PCa cells, which characterized high dose-responsiveness to androgen receptor (AR) its variants. Metabolome analysis of MALAT1-depleted cells was performed quantitative High-resolution 1 H-Nuclear Magnetic Resonance (NMR) spectroscopy. In addition, genomic regions were...
This review is based on novel observations from our laboratory the nuclear translocation and functional role of endothelial nitric oxide synthase (eNOS) in prostate cancer (PCa) epithelial cells. Nitric (NO), product eNOS, a free radical involved physiology pathophysiology living organisms variety biological processes including maintenance vascular homeostasis. Of relevance this context that estrogens play apoptotic process migration cells through regulation target genes such as eNOS itself....