Bovine Leukemia Virus (BLV) produces disorders on the immune system in naturally infected animals, which may counteract development of immunity after vaccination. The aim this study was to investigate whether healthy and BLV cattle elicited similar humoral responses foot mouth disease (FMD) immunization. In a field study, 35 Holstein heifers were selected based their serological status immunized with single dose commercial bivalent oil-based FMD vaccine. Serum samples collected at 0, 15, 60,...

Bovine-viral-diarrhea virus (BVDV) can cause significant economic losses in livestock. The disease is controlled with vaccination and bovines are susceptible until vaccine immunity develops may remain vulnerable if a persistently infected animal left on the farm; therefore, an antiviral agent that reduces infectivity be useful tool control programs. Although many compounds promising in-vitro efficacy have been identified, lack of laboratory-animal models limited their potential for further...

Infection of professional antigen presenting cells by viruses can have a marked effect on these and important consequences for the generation subsequent immune responses. In this study, we demonstrate that different strains bovine viral diarrhea virus (BVDV) infect dendritic differentiated from nonadherent peripheral monocytes (moDCs). BVDV did not cause apoptosis in cells. moDC was prevented incubating with anti-E2 antibodies or pretreating recombinant E2 protein before contact, suggesting...

We demonstrated recently that immunization with recombinant Neospora caninum profilin (rNcPRO) induces limited protection and a regulatory T-cell response in mice. The aim of this study was to evaluate the immune elicited by rNcPRO cattle assess strategy enhance its immunogenicity, combining addition epitopes modulators. developed chimeric fused functional present N-terminal sequence vesicular stomatitis virus (VSV) glycoprotein G (rNcPRO/G). Groups three were immunized two doses (2 weeks...

The efficacy of foot-and-mouth disease virus (FMDV) inactivated vaccines is mainly dependent on the integrity whole (146S) viral particles. If intact capsids disassemble to 12S subunits, antibodies against internal-not protective epitopes, may be induced. Serological correlates with protection hampered if internal epitopes are measured. Here we compared performance different ELISAs virus-neutralization test (VNT) that measures exposed epitopes. Sera from pigs immunized one dose an expired...

Interferon lambda (IFN-λ) is an antiviral naturally produced in response to viral infections, with activity on cells of epithelial origin and located the mucosal surfaces. This localized results reduced toxicity compared type I IFNs, whose receptors are ubiquitously expressed. IFN-λ has been effective therapy respiratory playing a crucial role potentiating adaptive immune responses that initiate at Human polymorphisms may cause differences interaction specific receptor human population....

The Coronavirus Disease 2019 (COVID-19) is a zoonotic disease caused by the pandemic virus SARS-CoV-2. Domestic and wild animals are susceptible to infection potential reservoirs for variants. To date, there no information about exposure of companion in Buenos Aires Suburbs, area with largest population Argentina where highest number COVID-19 human cases occurred during first wave. Here we developed multi-species indirect ELISA measure antibodies reactive SARS-CoV-2 receptor-binding domain...

Innate immune cells show enhanced responsiveness to secondary challenges after an initial non-related stimulation (Trained Immunity, TII). Acute NOD2 activation by Muramyl-Dipeptide (MDP) promotes TII inducing the secretion of pro-inflammatory mediators, while a sustained MDP-stimulation down-regulates inflammatory response, restoring tolerance. Here we characterized in-vitro response murine macrophages lipopolysaccharide (LPS) challenge under NOD2-chronic stimulation. RAW264.7 were trained...

Little is known about the influence of maternal antibodies and immune cells transferred through colostrum on responses calves to currently used foot-and-mouth disease (FMD) vaccines. Here we evaluated humoral cellular induced by vaccination colostrum-deprived that received equivalent amounts preparations differed in presence or absence but contained same quantity quality anti-foot-and-mouth virus (FMDV) antibodies. Three groups 32-d-old (n = 3 per group) were deprived fed either whole a...

Buffaloes are compulsory vaccinated against foot-and-mouth disease virus (FMDV) in many countries as part of the official control programmes. Serological testing aimed to indirectly assess herd immunity is currently performed using same Enzyme-linked immunosorbent assays (ELISAs) applied for bovine sera, assuming an agreement between ELISA's diagnostic results and those obtained neutralization test (VNT). Here we evaluated accuracy different ELISA tests vaccine-induced antibodies FMDV...

El objetivo de este trabajo fue conocer la seroprevalencia Neospora caninum en los bovinos tambos del Valle Lerma (Salta, Argentina) y factores riesgo asociados a transmisión parásito esta región. Se tomaron muestras suero aproximadamente 40 vacas cada tambo, que fueron analizadas por ELISA indirecto para detectar anticuerpos contra N. caninum. También se discriminó entre infecciones crónicas agudas midiendo avidez dichos anticuerpos. Todos presentaron al menos un bovino seropositivo media...

The role of water buffaloes in foot-and-mouth disease (FMD) epidemiology as one the major hosts virus that can develop persistent asymptomatic infection highlights importance sustaining surveillance on antibody response elicited by vaccination these animals. There is gap knowledge how serological assays measure antibodies against capsid proteins perform with buffalo samples and which would be most reliable test to substitute neutralization (VNT) a cumbersome low-throughput tool for field...