Organ regeneration in mammals is hypothesized to require a functional pool of stem or progenitor cells, but the role these cells lung unknown. Whereas postnatal alveolar tissue has been attributed type II epithelial (AECII), we reasoned that bronchioalveolar (BASCs) have potential contribute substantially this process. To test hypothesis, unilateral pneumonectomy (PNX) was performed on adult female C57/BL6 mice stimulate compensatory regrowth. The density BASCs and AECII, morphometric...

The objective of this study was to examine the relationship between chronic cough, pulmonary mechanical function, bronchial hyper-responsiveness, and peripheral airway inflammation in adult performance horses with nonseptic inflammatory disease (IAD).We hypothesized that a presenting complaint cough have higher percentage cells on bronchoalveolar lavage (BAL), greater obstruction, hyper-responsiveness (AHR) than do without cough. ANIMALS/SAMPLE POPULATION: Adult (n = 137) referred for...

While multipotent mesenchymal stromal cells have been recently isolated from adult lung (L-MSCs), there is very limited data on their biological properties and therapeutic potential in vivo. How L-MSCs compare with bone marrow-derived MSCs (BM-MSCs) also unclear. In this study, we characterized L-MSC phenotype, clonogenicity, differentiation potential, compared to BM-MSCs vivo survival, retention, paracrine gene expression, repair or elastase injury after transplantation. were highly...

Fecal microbial transplantation (FMT), a treatment for certain gastrointestinal conditions associated with dysbiosis in people, is also empirically employed horses colitis. This study used microbiota high-throughput sequencing to compare the fecal profile of healthy that geriatric transplant recipients experiencing diarrhea and tested whether FMT restores diversity.

SUMMARY Objective To correlate indices of airway reactivity to bronchoalveolar lavage (BAL) fluid cytologic features in horses with a recent decline exercise tolerance. Animals 20 actively working from 2 24 years old. Procedure Bronchoalveolar samples were obtained and analyzed. Forced oscillatory mechanics (1-7 Hz) technique was used for measurements total respiratory system resistance (R RS ), compliance (C resonant frequency (f res ). Changes R (1 during histamine challenge generate...

Autologous lung-derived mesenchymal stem cells (LMSCs) were transplanted endoscopically into sheep with experimental emphysema to assess their capacity regenerate functional tissue. LMSC lines derived from transbronchial biopsies, cloned at passage 2, expanded in culture, and labeled. A delivery scaffold containing 1% fibrinogen, 20 μg/ml of fibronectin, poly-L-lysine was used promote cell attachment spreading. Treatment animals received 5-10 × 10(6) cells/site; control alone. Phenotypic...

While aging leads to a reduction in the capacity for regeneration after pneumonectomy (PNX) most mammals, this biological phenomenon has not been characterized over lifetime of mice. We measured age-specific (3, 9, 24 month) effects PNX on physiology, morphometry, cell proliferation and apoptosis, global gene expression, lung fibroblast phenotype clonogenicity female C57BL6 The data show that only 3 month old mice were fully capable restoring volumes by day 7 total alveolar surface area 21...

Background and aims Fecal microbial transplantation (FMT) is empirically implemented in horses with colitis to facilitate resolution of diarrhea. The purpose this study was assess FMT as a clinical treatment modulator fecal microbiota hospitalized colitis. Methods A total 22 moderate severe diarrhea, consistent diagnosis colitis, were enrolled at two referral hospitals (L1: n = 12; L2: 10). performed all 12 patients on 3 consecutive days L1, while L2 consisted standard care without FMT....

Glucocorticosteroids remain the most common pharmaceutical approach for treatment of equine asthma but can be associated with significant side effects, including respiratory microbiome alterations. The goal study was to assess impact 2% lidocaine nebulization, a projected alternative asthma, on healthy microbiota. A prospective, randomized, controlled, blinded, 2-way crossover performed, effect 1 mg/kg (7 treatments over 4 days) microbiota compared control horses (saline and no treatment)....

Mechanical stress is an important modulator of lung morphogenesis, postnatal development, and compensatory regrowth. The effect mechanical on stem or progenitor cells unclear. We examined whether proliferative responses epithelial cells, including dually immunoreactive (CCSP proSP-C) (CCSP+/SP-C+) type II alveolar (ATII), are affected by physical factors found in the emphysematics, loss elastic recoil, reduced elastin content, destruction. Mice underwent single pneumonectomy (PNY) to...

Abstract Background Hemosiderophages in bronchoalveolar lavage fluid (BALF) are commonly ascribed to exercise‐induced pulmonary hemorrhage (EIPH). Little information exists regarding the presence of these cells horses that perform light or no work and referred for respiratory problems. Objectives Evaluate hemosiderophages BALF suspected disease without history risk factors EIPH determine predictors this population. Methods Observational retrospective cross‐sectional study using STROBE...

Diagnosis of inflammatory airway disease (IAD) currently rests upon the results bronchoalveolar lavage (BAL) cytology, lung function testing and histamine bronchoprovocation (HBP), none which provides direct information about structural change in lung.That thoracic radiography might better portray lungs therefore offer a good clinical assessment IAD.A radiographic scoring system was developed to assess extent bronchial interstitial pattern on radiographs dorsocaudal, dorsocranial...

Inflammatory airway disease (IAD) in horses, similar to asthma humans, is a common cause of chronic poor respiratory health and exercise intolerance due inflammation exaggerated constrictive responses. Human rhinovirus an important trigger for the development asthma; role viral equine IAD has not been established yet.In case-control study, horses with (n = 24) were compared control animals from comparable stabling environments 14). Horses classified using pulmonary function testing...

(1) Background: Equine asthma (EA) is a pervasive and important cause of poor performance respiratory morbidity in horses. Diagnosis EA includes an owner complaint, clinical scoring, lung function testing, cytological analysis bronchoalveolar lavage (BAL) cytology. There paucity information about the longitudinal course disease using these outcome assessments; thus, this study sought to describe quantify, horses with more than one visit specialty pulmonary clinic New England, type range...

Abstract Objective To compare response of horses to histamine bronchoprovocation (HBP), using total respiratory resistance (R rs ) measured by forced oscillatory mechanics (FOM) with dynamic compliance (C dyn and pulmonary L the esophageal balloon method. Animals 10 various degrees airway reactivity. Procedure The 2 methods for measuring responses HBP were performed on separate days. Endpoints compared increase 150 or 200% baseline R (PC , PC 200 150% decrease 65% C 65 ). Frequency...

Inflammatory airway disease has a high prevalence in horses, but is often diagnostic challenge. Flowmetric plethysmography and histamine bronchoprovocation (FP/HBP) simple effective tool for diagnosis, reproducibility of these measurements made over time not been established.We hypothesize that the measurement responsiveness horses using FP/HBP consistent both short long periods time.Twenty-nine healthy adult from 2 university herds.In this prospective experimental study, was determined each...

People working in cattle, swine and poultry barns have a higher prevalence of respiratory symptoms decreased lung function. There is scant evidence regarding the health humans horse barns, although it well documented that stabled horses high airway disease.To determine whether people spending time self-reported than non-exposed controls.A cross-sectional questionnaire study was conducted from May 2005 to January 2006 investigate 82 barn-exposed subjects 74 control subjects. Logistic...

Abstract Background Adult mice have a remarkable capacity to regenerate functional alveoli following either lung resection or injury that exceeds the regenerative observed in larger adult mammals. The molecular basis for this unique capability is largely unknown. We examined transcriptomic responses single pneumonectomy order elucidate prospective signaling mechanisms used species during regeneration. Methods Unilateral left sham thoracotomy was performed under general anesthesia (n = 8 per...

Background Despite the increasing number of geriatric horses attended by veterinarians, there is a lack understanding aging‐related changes on respiratory system horses. Objective To identify function and bronchoalveolar lavage fluid ( BALF ) cytology Animals Fifteen healthy young adult (2–11 years) 16 aged (≥20 Methods The was examined measurement arterial blood gases ABG ), use inductive plethysmography RIP for assessment breathing pattern ventilatory parameters, histamine...

Two adult female alpacas were evaluated for acute onset of weakness, pale mucous membranes, and signs depression unknown etiology. Both had intravascular hemolysis, anemia, Heinz body formation been fed wilted red maple leaves. Clinical developed several days after ingestion the No other toxin exposure was reported, no parasites detected. Dietary copper nitrate-nitrite concentrations determined to be within reference limits. continued become profoundly anemic with hemolysis. This...