A5076169487- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Synthesis and Characterization of Heterocyclic Compounds
- Synthesis of Organic Compounds
- Ubiquitin and proteasome pathways
- Wound Healing and Treatments
- Pluripotent Stem Cells Research
- Advanced Fluorescence Microscopy Techniques
- Thallium and Germanium Studies
- Signaling Pathways in Disease
- Kruppel-like factors research
- Oral and gingival health research
- Phytoestrogen effects and research
- Diet, Metabolism, and Disease
- Photoacoustic and Ultrasonic Imaging
- Optical Imaging and Spectroscopy Techniques
- Synthesis of heterocyclic compounds
- Cancer-related gene regulation
- Reproductive Biology and Fertility
- Extracellular vesicles in disease
- 14-3-3 protein interactions
- Research on Leishmaniasis Studies
- Polyamine Metabolism and Applications
- Cell Image Analysis Techniques
- Cholinesterase and Neurodegenerative Diseases
Seoul National University Hospital
2018-2022
Eulji University
2016-2019
University of Seoul
2016
Significance The Hippo-YAP/TAZ pathway plays pivotal roles in controlling tissue growth, and its abnormal regulation is frequently implicated a variety of cancers. We have identified MAML1/2 as critical regulators for YAP/TAZ nuclear retention transcription activities. Clinical analysis with specimens human cancer patient public database reveals pathological association between MAML expression YAP signature. Furthermore, our results suggest that the cell density governing subcellular...
Abstract Wnt signaling is mainly transduced by β-catenin via regulation of the destruction complex containing Axin, APC, and GSK3β. Transcription factor EB (TFEB) a well-known master regulator autophagy lysosomal biogenesis processes. TFEB’s nuclear localization transcriptional activity are also regulated various upstream signals. In this study, we found that induces TFEB expression target genes TFEB-β-catenin-TCF/LEF1 as well β-catenin-TCF/LEF1 complexes. Our biochemical data revealed part...
Recently, many studies have investigated the role of extracellular vesicles (EVs) on reproductive events, including embryo development and death, oviduct–embryo crosstalk, in vitro fertilization others. The aim this study was to demonstrate whether outgrowth embryo–derived EVs function as bioactive molecules regulate mouse embryonic developmental competence implantation potential utero. from embryos 7.5 days postcoitum were detected selectively isolated evaluate embryotrophic functions...
Abstract This study hypothesizes that the application of low‐dose nonthermal biocompatible dielectric barrier discharge plasma (DBD‐NBP) to human gingival fibroblasts (HGFs) will inhibit colony formation but not cell death and induce matrix metalloproteinase (MMP) expression, extracellular (ECM) degradation, subsequent migration, which can result in enhanced wound healing. HGFs treated with for 3 min migrate each other across gap faster than those control 5‐min treatment groups on days 1 3....
This study evaluated the synergistic effect of curcumin (diferuloylmethane) and hinokitiol (β-thujaplicin), natural product derived phytochemicals, on gefitinib (Iressa) resistant non-small cell lung cancer (NSCLC) cells. Gefitinib, a tyrosine kinase inhibitor targeting epidermal growth factor receptor (EGFR), is widely used for treatment. However, resistance easily acquired by NSCLC followed development progressive disease. Curcumin are well-known bioactive compounds demonstrating...
Cancer is genetically, metabolically and infectiously induced life threatening disorder showing aggressive growing pattern with invasive tendency. In order to prevent this global menace from jeopardizing human life, enormous studies on carcinogenesis treatment for chemotherapy resistance have been intensively researched. Hinokitiol (β-thujaplicin) extracted heart wood of cupressaceous a well-known bioactive compound demonstrating anti-inflammation, anti-bacteria anti-cancer effects several...
A microscope is the fundamental research and diagnostic apparatus for clinical investigation of signaling transduction, morphological changes physiological tracking cells intact tissues from patients in biomedical laboratory science. Proper use, care maintenance with comprehensive understanding mechanism are fully requested reliable image data accurate interpretation diagnosis laboratory. The standard operating procedure (SOP) light microscopes includes performance procedure, brief...
조직병리학에서 현미경을 이용한 삼차원적 접근법은, 이차원 단면의 조직 슬라이드에서 박절 과정 중 부차적으로 발생하는 공간정보의 손실로 인하여 확인하기 어려웠던, 내부 분자들의 공간적 배열, 상호결합, 구조적인 형태와 이들의 통합적인 정보체로서, 내에 복잡하게 얽혀진 다양한 정보를 풀어내는데 있어서 복합적인 데이터를 제시하여 준다. 이광자 현미경(two-photon microscope)과 자동화된 보정환 (correction collar)이 탑재된 고성능 대물렌즈의 개발과 같은 광학장비 영역의 발전은 조직투명화 과정을 거치지 않은 두꺼운 시료의 이미징에 광학적인 이론과 실체 사이에 존재하는 격차를 줄이는데 기여하였다고 할 수 있다. 하지만, 길어진 작동범위(working distance)와 최적화된 고강도 레이저의 사용으로 얻게 되는 이점들은 세포 내 각 구성요소의 굴절률(refractive index) 차이로 증가되는 빛의 분산(light scattering) 현상으로 인해...
The mammalian zygote cleaves and develops to blastocyst within the zona pellucida (ZP) in vivo. presence or absence of ZP may affect characteristics embryo, including blastomere alignment, cell-cell junction, compaction. This study aimed compare morphokinetic intact free mouse pre-implantation embryos with time-lapse monitoring system. Mouse 2-cell were collected 1.5 days post coitum (dpc), their ZPs removed by treatment acid Tyrode’s solution. All cultured vitro up outgrowth stage at 7.5...
Cordyceps militaris has been used in traditional Chinese medicine owing to its anticancer and immunomodulatory activities. Germanium compounds have also shown be associated with many pharmacological functions, such as antimicrobial, antiviral, antitumor, antimutagenic, immunomodulating effects. In this study, we examined the biological properties of hot water extract from mycelial liquid culture germanium-enriched C. (CMGe). CMGe displayed a concentration-dependent antiproliferation activity...
Jeong Su Choi, Sang Yong Park, Man Gil Yang, Dong Beom Lee, Tae Bok Ji Hye Heo, Min Woo and Suhng Wook Kim. Korean J Clin Lab Sci. 2016;48:169-75. https://doi.org/10.15324/kjcls.2016.48.3.169
Cell migration은 embryogenesis 혹은 cancer metastasis 이외에, 물리적 손상에 의한 상처의 수복을 위해서 손상된 부위로 세포가 이동하는 매우 흔하게 관찰되는 현상 중 하나 이다. Wound healing assay는 in vitro의 이차원 평면상에서 세포의 이동을 관찰할 수 있는 기본적인 연구 기법이다. In vitro상에서 손상을 재현하는 가장 손쉬운 접근법으로서, confluent monolayer 표면에 날카로운 도구를 이용하여 기계적인 스크레치를 내는 방법이 사용되고 있다. 완충 스프링이 탑재된 금속 핀을 96-well plate를 기반으로 하는 wound maker에 장착하여 multi-well plate 바닥 표면의 고르지 못한 굴곡과 스크레칭 팁 사이에 직각을 이루는 접촉면에서의 미세한 조절이 가능하도록 하였다. 실험용 팁으로 monolayer위에 스크래치를 내었을 때에는 다양한 지그재그 패턴이 그려진 반면에, 직접 제작한...
Oral Wound Healing In article 2200527 by Eun Ha Choi, Jae Jun Ryu, and co-workers, nonthermal biocompatible plasma (NBP) stimulates human gingival fibroblast (HGF) cell migration through focal adhesion kinase (FAK) pathway. NBP inhibits colony formation induces matrix metalloproteinase (MMP) expression, extracellular (ECM) degradation, subsequent migration. plays a role in motility to regulate AKT p21 signals HGF cells, actin modification of cytoskeleton.